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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei41SubstrateUniRule annotation1
Binding sitei74SubstrateUniRule annotation1
Metal bindingi96Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei124SubstrateUniRule annotation1
Active sitei126Proton donorUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Metal bindingi192Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei263SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1962-MONOMER
UniPathwayiUPA00056; UER00097
UPA00059; UER00105

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Short name:
HMBPP reductaseUniRule annotation
Gene namesi
Name:ispHUniRule annotation
Ordered Locus Names:CD630_18180
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000211051 – 2794-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST279

Interactioni

Protein-protein interaction databases

STRINGi272563.CD1818

Structurei

3D structure databases

ProteinModelPortaliQ187C6
SMRiQ187C6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IUH Bacteria
COG0761 LUCA
HOGENOMiHOG000220193
KOiK03527
OMAiHNKYVVD

Family and domain databases

CDDicd13944 lytB_ispH, 1 hit
HAMAPiMF_00191 IspH, 1 hit
InterProiView protein in InterPro
IPR003451 LytB/IspH
PANTHERiPTHR30426 PTHR30426, 1 hit
PfamiView protein in Pfam
PF02401 LYTB, 1 hit
TIGRFAMsiTIGR00216 ispH_lytB, 1 hit

Sequencei

Sequence statusi: Complete.

Q187C6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKIAKNAG FCFGVKRAMK MAWDEVEKND SGIYALGPLI HNKQAVAKYE
60 70 80 90 100
EKGLKTVNEI DTIPNHENMI IRSHGVPENI YKEAKDKKLK IVDTTCPFVK
110 120 130 140 150
KIHTVVSEYH NKGYEIIVIG DMKHPEVIGI NGWCENSAII IKTLEQMENM
160 170 180 190 200
EFDNSKRYCL VAQTTINPEL YISIVNKLSD KLEEIVFNDT ICSATKTRQE
210 220 230 240 250
SAKELAKEVD CMIVIGGKHS SNTQKLVKVC EDLVPTFAIE TKDELDVNTL
260 270
KKYKNLGITA GASTPNWIIE EVVTFLENL
Length:279
Mass (Da):31,466
Last modified:July 25, 2006 - v1
Checksum:iE4BD040BB1352D22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ68688.1
RefSeqiWP_003439450.1, NZ_CP010905.2
YP_001088323.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ68688; CAJ68688; CD630_18180
GeneIDi31353427
4914744
KEGGicdf:CD630_18180
pdc:CDIF630_02018
PATRICifig|272563.120.peg.1912

Similar proteinsi

Entry informationi

Entry nameiISPH_PEPD6
AccessioniPrimary (citable) accession number: Q187C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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