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Q186Z6 (ASSY_PEPD6) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:CD630_17850
OrganismPeptoclostridium difficile (strain 630) (Clostridium difficile) [Complete proteome] [HAMAP]
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 399399Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000263913

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site871Citrulline By similarity
Binding site921Citrulline By similarity
Binding site1171ATP; via amide nitrogen By similarity
Binding site1191Aspartate By similarity
Binding site1231Aspartate By similarity
Binding site1231Citrulline By similarity
Binding site1241Aspartate By similarity
Binding site1271Citrulline By similarity
Binding site1761Citrulline By similarity
Binding site1851Citrulline By similarity
Binding site2611Citrulline By similarity
Binding site2731Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q186Z6 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 611F4D7142226809

FASTA39944,624
        10         20         30         40         50         60 
MKEKVVLAYS GGLDTSIIIP WLKENYEDID VIAVCGNVGQ EDKMEDVYEK ALQSGASKAY 

        70         80         90        100        110        120 
VDDISEEFVT ETIFKAVKAE AKYEGKYLLG TSLARPIIAK KLVEVAHKEG AKYICHGCTG 

       130        140        150        160        170        180 
KGNDQVRFEA TIAALDPTIK VIAPWRIWDI KSREDAIDYA EKHNIKVTAT KAKIYSVDAN 

       190        200        210        220        230        240 
LWHVSTEGGD IEHLENEHKK DVYKQCVDPE DACDVAEYVE VYFEKGVPKK VNGEELSPVA 

       250        260        270        280        290        300 
LIHKLNELGC KHGIGVIDIV ENRLVGMKSR GIYETPGGTI LYEAHNILES ATLDKDTLHF 

       310        320        330        340        350        360 
KQMVSYKYGE LIYNGLWYCK LRESIDAFME QTQDNVTGTV KVKLYKGNIK PAGIFTENAL 

       370        380        390 
YDEGISSFGN SELYDHKDAE GFINLFTLPL KIRAMKAGK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM180355 Genomic DNA. Translation: CAJ68655.1.
RefSeqYP_001088291.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ186Z6.
SMRQ186Z6. Positions 4-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272563.CD1785.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ68655; CAJ68655; CD630_17850.
GeneID4914984.
KEGGcdf:CD630_17850.
PATRIC19441973. VBICloDif38397_1873.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAAPPEEAY.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycCDIF272563:GJFE-1974-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_PEPD6
AccessionPrimary (citable) accession number: Q186Z6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways