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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40Substrate; via amide nitrogenUniRule annotation1
Binding sitei116ATPUniRule annotation1
Binding sitei162ATPUniRule annotation1
Binding sitei189Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1739-MONOMER
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:CD630_16000
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003365501 – 262Hydroxyethylthiazole kinaseAdd BLAST262

Proteomic databases

PRIDEiQ186F4

Interactioni

Protein-protein interaction databases

STRINGi272563.CD1600

Structurei

3D structure databases

ProteinModelPortaliQ186F4
SMRiQ186F4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

Q186F4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNLIKDVKK LNPLVIHYTN NVTINDCANV TLAVGASPLM SFSYEEVEEM
60 70 80 90 100
VSVANSVVIN IGTMNSNMLD LFLLAGKAAN KYNKPVVLDP VGVFASKARA
110 120 130 140 150
ELTSRLLNEV KFSVVKGNVS EIKFIGGFNV RGKGVDSFDE EEDSTEIIRK
160 170 180 190 200
IAEKLECVVV ATGKIDIITN GKGTYKINNG TDKLKGITGT GCMTASLIAS
210 220 230 240 250
FMAVTENILE AATMGVLTMS LSGELANLNN PPIGTFKENL MNAIYQMDID
260
ALSKNSNIEF LN
Length:262
Mass (Da):28,291
Last modified:July 25, 2006 - v1
Checksum:i5E891DE5374BA09D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ68465.1
RefSeqiWP_011861269.1, NZ_CP010905.2
YP_001088101.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ68465; CAJ68465; CD630_16000
GeneIDi31353199
4913385
KEGGicdf:CD630_16000
pdc:CDIF630_01775
PATRICifig|272563.120.peg.1676

Similar proteinsi

Entry informationi

Entry nameiTHIM_PEPD6
AccessioniPrimary (citable) accession number: Q186F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 25, 2006
Last modified: May 23, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health