Reviewed, UniProtKB/Swiss-Prot Q186A6 (AROD_CLOD6)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate dehydratase
      Short name=3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	CD2217

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000325520
Sites
	Active site	144	1	Proton acceptor By similarity
	Active site	171	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q186A6-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 60B9943466E5C9AD	FASTA	255	28,876
	10 20 30 40 50 60 MKRKVQVKNI TIGEGRPKIC VPIIGKNKKD IIKEAKELKD ACLDIIEWRV DFFENVENIK 70 80 90 100 110 120 EVKEVLYELR SYIHDIPLLF TFRSVVEGGE KLISRDYYTT LNKEISNTGL VDLIDVELFM 130 140 150 160 170 180 GDEVIDEVVN FAHKKEVKVI ISNHDFNKTP KKEEIVSRLC RMQELGADLP KIAVMPQNEK 190 200 210 220 230 240 DVLVLLEATN EMFKIYADRP IITMSMSGMG VISRLCGEIF GSALTFGAAK SVSAPGQISF 250 KELNSVLNLL HKSIN

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AM180355 Genomic DNA. Translation: CAJ69102.1. Different initiation.

RefSeq

YP_001088731.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3JS3	X-ray	2.20	A/B/C/D	1-255	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q186A6.

Genome annotation databases

GeneID

4914552.

GenomeReviews

Gene locus CD2217 in contig AM180355_GR.

KEGG

cdf:CD2217.

NMPDR

fig|1496.1.peg.2644.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0710.

HOGENOM

HBG732926.

Family and domain databases

HAMAP

MF_00214. AroD.
[Tree]

InterPro

IPR018508. 3-dehydroquinate_DH_AS.
IPR013785. Aldolase_TIM.
IPR001381. DHquinase_I.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

Pfam

PF01487. DHquinase_I. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01093. aroD. 1 hit.

PROSITE

PS01028. DEHYDROQUINASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AROD_CLOD6

Accession

Primary (citable) accession number: Q186A6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	February 9, 2010
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)