Reviewed, UniProtKB/Swiss-Prot Q18680 (IPYR_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable inorganic pyrophosphatase 1
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	pyp-1
Synonyms:	uba-1
ORF Names:	C47E12.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase phosphate metabolic process Inferred from electronic annotation. Source: InterPro positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform b (identifier: Q18680-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform a (identifier: Q18680-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: Missing.
     70-100: RGSLTTAVSTSSSGAQRQMSANSERSLHTRL → MGLVLIAKRNKPLIAMMAAILFTVAVFLATR

Note: No experimental confirmation available.

Isoform c (identifier: Q18680-3)

The sequence of this isoform differs from the canonical sequence as follows:
     100-100: Missing.

Note: No experimental confirmation available.

Isoform d (identifier: Q18680-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Probable inorganic pyrophosphatase 1		PRO_0000137571
Sites
	Metal binding	239	1	Magnesium 1 By similarity
	Metal binding	244	1	Magnesium 1 By similarity
	Metal binding	244	1	Magnesium 2 By similarity
	Metal binding	276	1	Magnesium 1 By similarity
Natural variations
	Alternative sequence	1 – 115	115	Missing in isoform d.		VSP_009599
	Alternative sequence	1 – 69	69	Missing in isoform a.		VSP_009598
	Alternative sequence	70 – 100	31	RGSLT…LHTRL → MGLVLIAKRNKPLIAMMAAI LFTVAVFLATR in isoform a.		VSP_009600
	Alternative sequence	100	1	Missing in isoform c.		VSP_009601

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified March 1, 2004. Version 3. Checksum: D46E7694A7065927	FASTA	407	44,152
	10 20 30 40 50 60 MGCAVSQESA IATVSSSSNT ATTSTSSSNT SQKWATSRTS RPVTNVTQVS AIHTTSMDSG 70 80 90 100 110 120 SSTVQLPSPR GSLTTAVSTS SSGAQRQMSA NSERSLHTRL PLSETAVILQ SQAVKMSTGA 130 140 150 160 170 180 GDSAVYEAVE RGSLYSLDYR VYIKGPQGIV SPWHDIPLFA NKDKRVYNMI VEIPRWTNAK 190 200 210 220 230 240 MEMATKEPFS PIKQDEKKGV ARFVHNIFPH KGYIWNYGAL PQTWEDPNHV VPDTGAKGDN 250 260 270 280 290 300 DPIDVIEVGS KVAGRGAVLQ VKVLGTLALI DEGETDWKLV AIDVNDENAD KLNDIDDVEK 310 320 330 340 350 360 VYPGLLAASV EWFRNYKIPA GKPANEFAFN GEFKNREYAE KVIDETNEYW KTLIKEANPS 370 380 390 400 LNTVSRVPEA VHQGTDEAAA TAIGATPEHG ANAPLPGDVD KWHFVQG
	Isoform a. Checksum: 890AC7911145EAA5	FASTA	338	37,285
	10 20 30 40 50 60 MGLVLIAKRN KPLIAMMAAI LFTVAVFLAT RPLSETAVIL QSQAVKMSTG AGDSAVYEAV 70 80 90 100 110 120 ERGSLYSLDY RVYIKGPQGI VSPWHDIPLF ANKDKRVYNM IVEIPRWTNA KMEMATKEPF 130 140 150 160 170 180 SPIKQDEKKG VARFVHNIFP HKGYIWNYGA LPQTWEDPNH VVPDTGAKGD NDPIDVIEVG 190 200 210 220 230 240 SKVAGRGAVL QVKVLGTLAL IDEGETDWKL VAIDVNDENA DKLNDIDDVE KVYPGLLAAS 250 260 270 280 290 300 VEWFRNYKIP AGKPANEFAF NGEFKNREYA EKVIDETNEY WKTLIKEANP SLNTVSRVPE 310 320 330 AVHQGTDEAA ATAIGATPEH GANAPLPGDV DKWHFVQG
	Isoform c. Checksum: 63594675F8874BDE	FASTA	406	44,039
	10 20 30 40 50 60 MGCAVSQESA IATVSSSSNT ATTSTSSSNT SQKWATSRTS RPVTNVTQVS AIHTTSMDSG 70 80 90 100 110 120 SSTVQLPSPR GSLTTAVSTS SSGAQRQMSA NSERSLHTRP LSETAVILQS QAVKMSTGAG 130 140 150 160 170 180 DSAVYEAVER GSLYSLDYRV YIKGPQGIVS PWHDIPLFAN KDKRVYNMIV EIPRWTNAKM 190 200 210 220 230 240 EMATKEPFSP IKQDEKKGVA RFVHNIFPHK GYIWNYGALP QTWEDPNHVV PDTGAKGDND 250 260 270 280 290 300 PIDVIEVGSK VAGRGAVLQV KVLGTLALID EGETDWKLVA IDVNDENADK LNDIDDVEKV 310 320 330 340 350 360 YPGLLAASVE WFRNYKIPAG KPANEFAFNG EFKNREYAEK VIDETNEYWK TLIKEANPSL 370 380 390 400 NTVSRVPEAV HQGTDEAAAT AIGATPEHGA NAPLPGDVDK WHFVQG
	Isoform d. Checksum: F25C90F59D7C8D16	FASTA	292	32,363
	10 20 30 40 50 60 MSTGAGDSAV YEAVERGSLY SLDYRVYIKG PQGIVSPWHD IPLFANKDKR VYNMIVEIPR 70 80 90 100 110 120 WTNAKMEMAT KEPFSPIKQD EKKGVARFVH NIFPHKGYIW NYGALPQTWE DPNHVVPDTG 130 140 150 160 170 180 AKGDNDPIDV IEVGSKVAGR GAVLQVKVLG TLALIDEGET DWKLVAIDVN DENADKLNDI 190 200 210 220 230 240 DDVEKVYPGL LAASVEWFRN YKIPAGKPAN EFAFNGEFKN REYAEKVIDE TNEYWKTLIK 250 260 270 280 290 EANPSLNTVS RVPEAVHQGT DEAAATAIGA TPEHGANAPL PGDVDKWHFV QG

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z68882 Genomic DNA. Translation: CAA93107.3. Z68882 Genomic DNA. Translation: CAD89726.1. Z68882 Genomic DNA. Translation: CAD89727.1. Z68882 Genomic DNA. Translation: CAD89728.1.
PIR	E88797. T20014.
RefSeq	NP_001023073.1. NP_001023074.1. NP_001023075.1. NP_001023076.1.
UniGene	Cel.8431
3D structure databases
HSSP	HSSP built from PDB template 1WGI based on UniProtKB P00817.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q18680. 1 interaction.
STRING	Q18680.
Proteomic databases
PRIDE	Q18680.
Genome annotation databases
Ensembl	C47E12.4a.1; C47E12.4a.1; C47E12.4; Caenorhabditis elegans. [Genome view] C47E12.4a.2; C47E12.4a.2; C47E12.4; Caenorhabditis elegans. [Genome view] C47E12.4b; C47E12.4b; C47E12.4; Caenorhabditis elegans. [Genome view] C47E12.4c.1; C47E12.4c.1; C47E12.4; Caenorhabditis elegans. [Genome view] C47E12.4c.2; C47E12.4c.2; C47E12.4; Caenorhabditis elegans. [Genome view] C47E12.4c.3; C47E12.4c.3; C47E12.4; Caenorhabditis elegans. [Genome view] C47E12.4d.1; C47E12.4d.1; C47E12.4; Caenorhabditis elegans. [Genome view] C47E12.4d.2; C47E12.4d.2; C47E12.4; Caenorhabditis elegans. [Genome view] C47E12.4d.3; C47E12.4d.3; C47E12.4; Caenorhabditis elegans. [Genome view]
GeneID	177856.
KEGG	cel:C47E12.4.
UCSC	C47E12.5a.1. c. elegans.
Organism-specific databases
CTD	177856.
WormBase	WBGene00008149. pyp-1.
WormPep	C47E12.4a. CE33767. [WorfDB] C47E12.4b. CE33768. [WorfDB] C47E12.4c. CE33769. [WorfDB] C47E12.4d. CE05448. [WorfDB]
Enzyme and pathway databases
BRENDA	3.6.1.1. 672.
Gene expression databases
ArrayExpress	Q18680.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	898676.

Entry information

Entry name

IPYR_CAEEL

Accession

Primary (citable) accession number: Q18680
Secondary accession number(s): Q86DB1, Q86DB2, Q86DB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	March 1, 2004
Last modified:	October 13, 2009
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project