Reviewed, UniProtKB/Swiss-Prot Q18678 (SYSC_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable seryl-tRNA synthetase, cytoplasmic
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS

Gene names

Name:	srs-2
ORF Names:	C47E12.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase seryl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Probable seryl-tRNA synthetase, cytoplasmic		PRO_0000122194
Regions
	Nucleotide binding	302 – 304	3	ATP By similarity
	Nucleotide binding	391 – 394	4	ATP By similarity
	Region	271 – 273	3	Serine binding By similarity
Sites
	Binding site	318	1	ATP; via carbonyl oxygen and amide nitrogen By similarity
	Binding site	325	1	Serine By similarity
	Binding site	429	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18678-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EC4AA5B811E5BB07	FASTA	487	55,220
	10 20 30 40 50 60 MVLDIDMFRT EKGGNPEIIR KSQQDRFKDP KLVDEVIELD EKWRKERFVA DQLNRQKNAI 70 80 90 100 110 120 SKAIGEKMKK KEPQGTDDSV ADDIVARLAE LKIDELSQLT VVQLKKLRVL VDEKSVQTAA 130 140 150 160 170 180 AVIANENARH EKLIQIGNLI HSSVVVSKDE ANNKIERTFG DLSTKKKYSH VDLVVMVDGF 190 200 210 220 230 240 DGERGTVVAG GRGYFLKGPL VFLEQAIIQL ALQRLNVKGY VPLYTPFFMR KEVMQEVAQL 250 260 270 280 290 300 SQFDDELYKV SSKGSEIAGD TSVDEKYLIA TSEQPIAAYH RNEWIKETEL PIKYAGVSTC 310 320 330 340 350 360 FRQEVGSHGR DTRGIFRVHQ FEKIEQFVLC SPNDNESWTL FDEMIGNAES YYQELQIPYQ 370 380 390 400 410 420 VVNIVSGELN NAAAKKFDLE AWFPGSGAYR ELVSCSNCLD YQSRRLKVRY GQTKKLSGEV 430 440 450 460 470 480 PFVHMLNATM CATTRVICAI LENNQTEEGI NVPTAIQQWM PENYRTFIPF VKPAPIDEDA KKATGKK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z68882 Genomic DNA. Translation: CAA93105.1.
PIR	T20008.
RefSeq	NP_501804.1.
UniGene	Cel.6509
3D structure databases
HSSP	HSSP built from PDB template 1SER based on UniProtKB P34945.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18678.
Genome annotation databases
Ensembl	C47E12.1.1; C47E12.1.1; C47E12.1; Caenorhabditis elegans. [Genome view] C47E12.1.2; C47E12.1.2; C47E12.1; Caenorhabditis elegans. [Genome view]
GeneID	177859.
KEGG	cel:C47E12.1.
UCSC	C47E12.1.1. c. elegans.
Organism-specific databases
CTD	177859.
WormBase	WBGene00005663. srs-2.
WormPep	C47E12.1. CE05445. [WorfDB]
Phylogenomic databases
OMA	MCATTRV.
Enzyme and pathway databases
BRENDA	6.1.1.11. 672.
Gene expression databases
ArrayExpress	Q18678.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	898694.

Entry information

Entry name

SYSC_CAEEL

Accession

Primary (citable) accession number: Q18678

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project