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Protein

Neprilysin-1

Gene

nep-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable cell surface protease. Required to control the neuronal innervation of pharyngeal pumping.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi587 – 5871Zinc; catalyticPROSITE-ProRule annotation
Active sitei588 – 5881PROSITE-ProRule annotation
Metal bindingi591 – 5911Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi649 – 6491Zinc; catalyticPROSITE-ProRule annotation
Active sitei653 – 6531Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM13.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Neprilysin-1 (EC:3.4.24.-)
Gene namesi
Name:nep-1
ORF Names:ZK20.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiZK20.6; CE39118; WBGene00013926; nep-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2723Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Neprilysin-1PRO_0000248421Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence analysis
Glycosylationi81 – 811N-linked (GlcNAc...)1 Publication
Glycosylationi132 – 1321N-linked (GlcNAc...)1 Publication
Glycosylationi217 – 2171N-linked (GlcNAc...)1 Publication
Glycosylationi273 – 2731N-linked (GlcNAc...)1 Publication
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)1 Publication
Glycosylationi612 – 6121N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ18673.
PaxDbiQ18673.

Expressioni

Tissue specificityi

Specifically expressed in pharyngeal cells and a single head neuron.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.ZK20.6.2.

Structurei

3D structure databases

ProteinModelPortaliQ18673.
SMRiQ18673. Positions 66-754.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOGENOMiHOG000245574.
InParanoidiQ18673.
OMAiSNTIQSR.
PhylomeDBiQ18673.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 2 hits.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTRFGPPIV FLISCYALIL CGTVDALPRA PYFNDDINKT TTTSEDKTVG
60 70 80 90 100
NTVVEEEKKT YTVGDSEGYQ EASRLLQKSL NLSLDPCDDF FEYACRAWVD
110 120 130 140 150
SHPIPDDLTS YSQFTATREK VLAEMRKLYE DNTSIPTSKS IALIKQIYNT
160 170 180 190 200
CMDTEKHNAV GARDLLEKIK TYGYWPMVHN EKWRESTFDL TKLLSNTIQS
210 220 230 240 250
RDVSVFFDFG PAEDSRNVSR RLLSFDQGSL GLGYSTRDYY LDEKKYEKQM
260 270 280 290 300
KAYRKYTIGK VRYYTEDAGM AVNESKIESD VDEIIAFEKE WAQILVAEED
310 320 330 340 350
RRNYTKLYNV RRFDDLKEYM SIIDWKKLTL STTPFLVHSY LKTNPSIIIS
360 370 380 390 400
DVEYLQKMNT LLQNTDPRIV TNYILLRWAG SWSQEIGKKY EDLQQEFAFQ
410 420 430 440 450
MYGRKQRQPR WKDCVSSAGG KLSYASGSMY VRKYFDANAK NTTLDMITDL
460 470 480 490 500
QEAFRNMMHA NDWMDAETKK YALEKADQML KQIGYPDFIL NDEKLDDWYK
510 520 530 540 550
GLEGAPEDSF SQLVEKSIQW RNNFYYRRLL EPVNRFEFIS SAAVVNAFYS
560 570 580 590 600
PTRNAIAFPA GILQQPFFDA RFPKALNYGG IGAVIGHEIT HGFDDTGRQF
610 620 630 640 650
DNVGNLRDWW DNTTSSKFNE RTQCIIEQYA DVKLRGTDLR INGKLTQGEN
660 670 680 690 700
IADNGGIKQA FKAYKSYLEK HGGQEARLPQ FESLTNEQLF FVGYAQVWCG
710 720 730 740 750
AKTPETKTLL LLTDPHSPET ARVNTVLTNQ PEFAEAFKCP AGSPMNPTKR

CVVW
Length:754
Mass (Da):86,945
Last modified:October 11, 2005 - v3
Checksum:i49447968E4D15860
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69904, Z69902 Genomic DNA. Translation: CAA93782.2.
PIRiT20003.
RefSeqiNP_496490.2. NM_064089.5.
UniGeneiCel.5167.

Genome annotation databases

EnsemblMetazoaiZK20.6; ZK20.6; WBGene00013926.
GeneIDi174787.
KEGGicel:CELE_ZK20.6.
UCSCiZK20.6.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69904, Z69902 Genomic DNA. Translation: CAA93782.2.
PIRiT20003.
RefSeqiNP_496490.2. NM_064089.5.
UniGeneiCel.5167.

3D structure databases

ProteinModelPortaliQ18673.
SMRiQ18673. Positions 66-754.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.ZK20.6.2.

Protein family/group databases

MEROPSiM13.013.

Proteomic databases

EPDiQ18673.
PaxDbiQ18673.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK20.6; ZK20.6; WBGene00013926.
GeneIDi174787.
KEGGicel:CELE_ZK20.6.
UCSCiZK20.6.1. c. elegans.

Organism-specific databases

CTDi174787.
WormBaseiZK20.6; CE39118; WBGene00013926; nep-1.

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOGENOMiHOG000245574.
InParanoidiQ18673.
OMAiSNTIQSR.
PhylomeDBiQ18673.

Miscellaneous databases

NextBioi885504.
PROiQ18673.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 2 hits.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Caenorhabditis elegans neprilysin NEP-1: an effector of locomotion and pharyngeal pumping."
    Spanier B., Stuerzenbaum S.R., Holden-Dye L.M., Baumeister R.
    J. Mol. Biol. 352:429-437(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81; ASN-132; ASN-217; ASN-273; ASN-441 AND ASN-612, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiNEPL1_CAEEL
AccessioniPrimary (citable) accession number: Q18673
Secondary accession number(s): Q23453
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 11, 2005
Last modified: March 16, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.