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Protein

Uridylate kinase

Gene

pyrH

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP.UniRule annotation

Catalytic activityi

ATP + UMP = ADP + UDP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP. Inhibited by UTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54UMP; via amide nitrogenUniRule annotation1
Binding sitei55ATP; via amide nitrogenUniRule annotation1
Binding sitei59ATPUniRule annotation1
Binding sitei72UMPUniRule annotation1
Binding sitei161ATPUniRule annotation1
Binding sitei166ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei169ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15ATPUniRule annotation4
Nucleotide bindingi133 – 140UMPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processPyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-2295-MONOMER
UniPathwayiUPA00159; UER00275

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinaseUniRule annotation (EC:2.7.4.22UniRule annotation)
Short name:
UKUniRule annotation
Alternative name(s):
Uridine monophosphate kinaseUniRule annotation
Short name:
UMP kinaseUniRule annotation
Short name:
UMPKUniRule annotation
Gene namesi
Name:pyrHUniRule annotation
Ordered Locus Names:CD630_21380
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000539121 – 234Uridylate kinaseAdd BLAST234

Proteomic databases

PRIDEiQ185S7

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD2138

Structurei

3D structure databases

ProteinModelPortaliQ185S7
SMRiQ185S7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 25Involved in allosteric activation by GTPUniRule annotation6

Sequence similaritiesi

Belongs to the UMP kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C41 Bacteria
COG0528 LUCA
HOGENOMiHOG000047187
KOiK09903
OMAiPIIVFDM

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_01220_B PyrH_B, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR011817 Uridylate_kinase
IPR015963 Uridylate_kinase_bac
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF005650 Uridylate_kin, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR02075 pyrH_bact, 1 hit

Sequencei

Sequence statusi: Complete.

Q185S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKPMYKRVL LKLSGEALAG ERGFGINNDV VNDIAIAIKK IQEIGVEVAV
60 70 80 90 100
VVGGGNFWRG RTSEGMDRTT ADYIGMLATV MNAMALQDAL ENIDVATRVQ
110 120 130 140 150
TAIDMRQIAE PYIRRRAVRH LEKERVVIFG AGTGNPYFTT DTTAALRAAE
160 170 180 190 200
MEAEVILLAK NVDAVYDKDP KVHADAKKFT ELSYMEVIQK ELKVMDSTAT
210 220 230
SLCMDNKIPI KVFELTTENI IRAVKGENIG TTVK
Length:234
Mass (Da):25,828
Last modified:July 25, 2006 - v1
Checksum:i24C90BBF3CE595B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ69023.1
RefSeqiWP_003430597.1, NZ_CP010905.2
YP_001088652.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ69023; CAJ69023; CD630_21380
GeneIDi31353732
4913118
KEGGicdf:CD630_21380
pdc:CDIF630_02369
PATRICifig|272563.120.peg.2258

Similar proteinsi

Entry informationi

Entry nameiPYRH_PEPD6
AccessioniPrimary (citable) accession number: Q185S7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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