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Protein

Inactive angiotensin-converting enzyme-related protein

Gene

acn-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inactive as a metallopeptidase, due to a lack of active site residues (PubMed:14559923). Required for larval molting, male tail development, and formation of adult alae (PubMed:14559923). Acts in the heterochronic pathway and plays a role in the developmental timing of postembryonic hypodermal seam cell division and adult alae production (PubMed:28933985). Acts synergistically with apl-1 in let-7 regulated postembryonic cell division events (PubMed:28933985). Might act downstream of the heterochronic protein lin-41 (PubMed:28933985). Negative regulator of lifespan, heat and oxidative stress response and age-related degenerative changes like reduced pharyngeal pumping and decreased body movements (PubMed:26918946). Lifespan restriction is dependent on the forkhead-type transcription factor daf-16 (PubMed:26918946).3 Publications

GO - Biological processi

  • ecdysis, collagen and cuticulin-based cuticle Source: WormBase
  • nematode male tail tip morphogenesis Source: WormBase
  • plasma membrane fusion Source: WormBase

Keywordsi

Molecular functionDevelopmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive angiotensin-converting enzyme-related protein
Alternative name(s):
ACE-like non-metallopeptidase protein 1
Gene namesi
Name:acn-1
ORF Names:C42D8.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC42D8.5a ; CE30627 ; WBGene00000039 ; acn-1
C42D8.5b ; CE37212 ; WBGene00000039 ; acn-1

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in the extension of mean and maximum lifespan of 21% and 18%, respectively, and increased resistance to oxidative stress caused by paraquat (PubMed:26918946). RNAi-mediated knockdown in a daf-16 mutant background shortens mean and maximum lifespan by 3% and 2%, respectively (PubMed:26918946). RNAi-mediated knockdown in a let-7 mutant background partially suppresses the retarded and supernumerary hypodermal seam cell divisions, retarded alae production and postembryonic lethality (PubMed:28933985). Double RNAi-mediated knockdown with apl-1 in a let-7 mutant phenotype leads to complete suppression of the heterochronic seam cell defects (PubMed:28933985).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002856720 – 906Inactive angiotensin-converting enzyme-related proteinAdd BLAST887

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi159N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi653N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ18581
PaxDbiQ18581
PeptideAtlasiQ18581
PRIDEiQ18581

PTM databases

iPTMnetiQ18581

Expressioni

Tissue specificityi

Expressed in the hypodermis, in the vulva during organogenesis, and in the ray papillae of the male tail.1 Publication

Developmental stagei

Expressed in embryos and larvae (PubMed:14559923). Expressed in an oscillating expression pattern during larval development, with low expression at the L2 stage, high expression at the L2/L3 transition and low expression at the L3 stage (PubMed:28933985).2 Publications

Gene expression databases

BgeeiWBGene00000039

Interactioni

Protein-protein interaction databases

IntActiQ18581, 1 interactor
MINTiQ18581
STRINGi6239.C42D8.5a.1

Structurei

3D structure databases

ProteinModelPortaliQ18581
SMRiQ18581
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi24 – 93Pro-richAdd BLAST70

Sequence similaritiesi

Belongs to the peptidase M2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3690 Eukaryota
ENOG410XPJ3 LUCA
GeneTreeiENSGT00520000055576
HOGENOMiHOG000020340
InParanoidiQ18581
KOiK01283
OMAiDFLTAHH
OrthoDBiEOG091G01SM
PhylomeDBiQ18581

Family and domain databases

CDDicd06461 M2_ACE, 1 hit
InterProiView protein in InterPro
IPR001548 Peptidase_M2
PANTHERiPTHR10514 PTHR10514, 1 hit
PfamiView protein in Pfam
PF01401 Peptidase_M2, 1 hit
PRINTSiPR00791 PEPDIPTASEA

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q18581-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKFHILLLLL VGACLPVFTQ EIKPKPELLP ADEAPKDPEA VFSEGEPFEL
60 70 80 90 100
TDALDTPKNG SVPVPEPEPK PEPEPEPEPK PEPEPSPTPE PEPAIKFDNI
110 120 130 140 150
ESEDYGDVAE TAASTQPDEL NTEVIEQLVD TFLNTGSIAS NKTNKGPVFA
160 170 180 190 200
NPVAQALVNS SNYWKTDNLQ APGSIKDEEK LRSWLAGYEA EAIKVLREVA
210 220 230 240 250
LSGWRYFNDA SPSLKLALDE AENVLTMFVR STSMQAKQFD MASVTDEKVM
260 270 280 290 300
RQLGYVSFEG MSALAPSRFA DYSQAQAALN RDSKDSTICD KDVPPPCALQ
310 320 330 340 350
KIDMDSIFRN EKDASRLQHL WVSYVTAIAK SKPSYNNIIT ISNEGAKLNG
360 370 380 390 400
FANGGAMWRS AFDMSSKVHK AEFDLNKQID KIYSTIQPFY QLLHAYMRRQ
410 420 430 440 450
LAGIYSNPVG LSKDGPIPAH LFGSLDGGDW SAHYEQTKPF EEESETPEAM
460 470 480 490 500
LSAFNTQNYT TKKMFVTAYR YFKSAGFPHL PKSYWTSSIF ARVWSKDMIC
510 520 530 540 550
HPAAALDMRA PNDFRVKACA QLGEPDFEQA HSLLVQTYYQ YLYKDQSLLF
560 570 580 590 600
REQASPVITD AIANAFAHLS TNPHYLYSQK LVPSEHLDIK DSVIINKLYK
610 620 630 640 650
ESLESFTKLP FTIAADNWRY ELFDGTVPKN KLNDRWWEIR NKYEGVRSPQ
660 670 680 690 700
PYNTSNLDAL IHNSVSQVHS PATRTLISYV LKFQILKALC PEGTILSEGC
710 720 730 740 750
ILSEDTTEKL RETMKLGSSI TWLKALEMIS GKGELDAQPL LEYYEPLINW
760 770 780 790 800
LRNTNEIDQV VVGWDGEGTP FTVEEIPKTR QPGDGGNGLP SEDRVAFPGG
810 820 830 840 850
ECVNGQECLL DSHCNGTICV CNDGLYTLEI GNTFNCVPGN PADSGFGDGK
860 870 880 890 900
GGLVIGLFNN EVTTPEPSAE PEPTAKTTTK MPPRVRAATS PFSLYLTVLL

IIYFAL
Length:906
Mass (Da):100,725
Last modified:October 1, 2002 - v2
Checksum:i50B3CD37EA62CBD3
GO
Isoform b (identifier: Q18581-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-197: SSNYWKTDNL...YEAEAIKVLR → LTLNHFSSTT...TEKELHSLCS
     198-773: Missing.

Note: No experimental confirmation available.
Show »
Length:332
Mass (Da):35,399
Checksum:iDF2528B20E9D6CF6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015490160 – 197SSNYW…IKVLR → LTLNHFSSTTSHSSTGSAIP MKSIRLLLDGTEKELHSLCS in isoform b. CuratedAdd BLAST38
Alternative sequenceiVSP_015491198 – 773Missing in isoform b. CuratedAdd BLAST576

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080659 Genomic DNA Translation: CCD65566.1
FO080659 Genomic DNA Translation: CCD65567.1
PIRiT15792
RefSeqiNP_001024453.1, NM_001029282.3 [Q18581-1]
NP_001024454.1, NM_001029283.3 [Q18581-2]
UniGeneiCel.38713

Genome annotation databases

EnsemblMetazoaiC42D8.5a.1; C42D8.5a.1; WBGene00000039 [Q18581-1]
C42D8.5a.2; C42D8.5a.2; WBGene00000039 [Q18581-1]
GeneIDi180780
KEGGicel:CELE_C42D8.5
UCSCiC42D8.5b.1 c. elegans [Q18581-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACN1_CAEEL
AccessioniPrimary (citable) accession number: Q18581
Secondary accession number(s): Q65ZH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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