Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q18581

- ACN1_CAEEL

UniProt

Q18581 - ACN1_CAEEL

Protein

Inactive angiotensin-converting enzyme-related protein

Gene

acn-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (01 Oct 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for larval molting, male tail development, and formation of adult alae. Inactive as a metallopeptidase, due to a lack of active site residues.1 Publication

    GO - Molecular functioni

    1. metallopeptidase activity Source: InterPro
    2. peptidyl-dipeptidase activity Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inactive angiotensin-converting enzyme-related protein
    Alternative name(s):
    ACE-like non-metallopeptidase protein 1
    Gene namesi
    Name:acn-1
    ORF Names:C42D8.5
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome X

    Organism-specific databases

    WormBaseiC42D8.5a; CE30627; WBGene00000039; acn-1.
    C42D8.5b; CE37212; WBGene00000039; acn-1.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 906887Inactive angiotensin-converting enzyme-related proteinPRO_0000028567Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi159 – 1591N-linked (GlcNAc...)2 Publications
    Glycosylationi653 – 6531N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ18581.
    PRIDEiQ18581.

    Expressioni

    Tissue specificityi

    Expressed in embryonic and larval hypodermis, in the vulva during organogenesis, and in the ray papillae of the male tail.1 Publication

    Interactioni

    Protein-protein interaction databases

    IntActiQ18581. 1 interaction.
    MINTiMINT-3385438.
    STRINGi6239.C42D8.5a.

    Structurei

    3D structure databases

    ProteinModelPortaliQ18581.
    SMRiQ18581. Positions 177-764.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi24 – 9370Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG71044.
    GeneTreeiENSGT00520000055576.
    HOGENOMiHOG000020340.
    InParanoidiQ18581.
    KOiK01283.
    OMAiRDGANEG.
    PhylomeDBiQ18581.

    Family and domain databases

    InterProiIPR006149. EB_dom.
    IPR001548. Peptidase_M2.
    [Graphical view]
    PANTHERiPTHR10514. PTHR10514. 1 hit.
    PfamiPF01683. EB. 1 hit.
    PF01401. Peptidase_M2. 1 hit.
    [Graphical view]
    PRINTSiPR00791. PEPDIPTASEA.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q18581-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKFHILLLLL VGACLPVFTQ EIKPKPELLP ADEAPKDPEA VFSEGEPFEL    50
    TDALDTPKNG SVPVPEPEPK PEPEPEPEPK PEPEPSPTPE PEPAIKFDNI 100
    ESEDYGDVAE TAASTQPDEL NTEVIEQLVD TFLNTGSIAS NKTNKGPVFA 150
    NPVAQALVNS SNYWKTDNLQ APGSIKDEEK LRSWLAGYEA EAIKVLREVA 200
    LSGWRYFNDA SPSLKLALDE AENVLTMFVR STSMQAKQFD MASVTDEKVM 250
    RQLGYVSFEG MSALAPSRFA DYSQAQAALN RDSKDSTICD KDVPPPCALQ 300
    KIDMDSIFRN EKDASRLQHL WVSYVTAIAK SKPSYNNIIT ISNEGAKLNG 350
    FANGGAMWRS AFDMSSKVHK AEFDLNKQID KIYSTIQPFY QLLHAYMRRQ 400
    LAGIYSNPVG LSKDGPIPAH LFGSLDGGDW SAHYEQTKPF EEESETPEAM 450
    LSAFNTQNYT TKKMFVTAYR YFKSAGFPHL PKSYWTSSIF ARVWSKDMIC 500
    HPAAALDMRA PNDFRVKACA QLGEPDFEQA HSLLVQTYYQ YLYKDQSLLF 550
    REQASPVITD AIANAFAHLS TNPHYLYSQK LVPSEHLDIK DSVIINKLYK 600
    ESLESFTKLP FTIAADNWRY ELFDGTVPKN KLNDRWWEIR NKYEGVRSPQ 650
    PYNTSNLDAL IHNSVSQVHS PATRTLISYV LKFQILKALC PEGTILSEGC 700
    ILSEDTTEKL RETMKLGSSI TWLKALEMIS GKGELDAQPL LEYYEPLINW 750
    LRNTNEIDQV VVGWDGEGTP FTVEEIPKTR QPGDGGNGLP SEDRVAFPGG 800
    ECVNGQECLL DSHCNGTICV CNDGLYTLEI GNTFNCVPGN PADSGFGDGK 850
    GGLVIGLFNN EVTTPEPSAE PEPTAKTTTK MPPRVRAATS PFSLYLTVLL 900
    IIYFAL 906
    Length:906
    Mass (Da):100,725
    Last modified:October 1, 2002 - v2
    Checksum:i50B3CD37EA62CBD3
    GO
    Isoform b (identifier: Q18581-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         160-197: SSNYWKTDNL...YEAEAIKVLR → LTLNHFSSTT...TEKELHSLCS
         198-773: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:332
    Mass (Da):35,399
    Checksum:iDF2528B20E9D6CF6
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei160 – 19738SSNYW…IKVLR → LTLNHFSSTTSHSSTGSAIP MKSIRLLLDGTEKELHSLCS in isoform b. CuratedVSP_015490Add
    BLAST
    Alternative sequencei198 – 773576Missing in isoform b. CuratedVSP_015491Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080659 Genomic DNA. Translation: CCD65566.1.
    FO080659 Genomic DNA. Translation: CCD65567.1.
    PIRiT15792.
    RefSeqiNP_001024453.1. NM_001029282.3. [Q18581-1]
    NP_001024454.1. NM_001029283.3. [Q18581-2]
    UniGeneiCel.38713.

    Genome annotation databases

    EnsemblMetazoaiC42D8.5a; C42D8.5a; WBGene00000039. [Q18581-1]
    GeneIDi180780.
    KEGGicel:CELE_C42D8.5.
    UCSCiC42D8.5b.1. c. elegans. [Q18581-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080659 Genomic DNA. Translation: CCD65566.1 .
    FO080659 Genomic DNA. Translation: CCD65567.1 .
    PIRi T15792.
    RefSeqi NP_001024453.1. NM_001029282.3. [Q18581-1 ]
    NP_001024454.1. NM_001029283.3. [Q18581-2 ]
    UniGenei Cel.38713.

    3D structure databases

    ProteinModelPortali Q18581.
    SMRi Q18581. Positions 177-764.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q18581. 1 interaction.
    MINTi MINT-3385438.
    STRINGi 6239.C42D8.5a.

    Proteomic databases

    PaxDbi Q18581.
    PRIDEi Q18581.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C42D8.5a ; C42D8.5a ; WBGene00000039 . [Q18581-1 ]
    GeneIDi 180780.
    KEGGi cel:CELE_C42D8.5.
    UCSCi C42D8.5b.1. c. elegans. [Q18581-1 ]

    Organism-specific databases

    CTDi 180780.
    WormBasei C42D8.5a ; CE30627 ; WBGene00000039 ; acn-1.
    C42D8.5b ; CE37212 ; WBGene00000039 ; acn-1.

    Phylogenomic databases

    eggNOGi NOG71044.
    GeneTreei ENSGT00520000055576.
    HOGENOMi HOG000020340.
    InParanoidi Q18581.
    KOi K01283.
    OMAi RDGANEG.
    PhylomeDBi Q18581.

    Miscellaneous databases

    NextBioi 910922.
    PROi Q18581.

    Family and domain databases

    InterProi IPR006149. EB_dom.
    IPR001548. Peptidase_M2.
    [Graphical view ]
    PANTHERi PTHR10514. PTHR10514. 1 hit.
    Pfami PF01683. EB. 1 hit.
    PF01401. Peptidase_M2. 1 hit.
    [Graphical view ]
    PRINTSi PR00791. PEPDIPTASEA.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    2. "An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensin-converting enzyme family that lacks a metallopeptidase active site."
      Brooks D.R., Appleford P.J., Murray L., Isaac R.E.
      J. Biol. Chem. 278:52340-52346(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    3. "Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins."
      Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T.
      Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-159, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.
    4. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-159 AND ASN-653, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiACN1_CAEEL
    AccessioniPrimary (citable) accession number: Q18581
    Secondary accession number(s): Q65ZH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3