Reviewed, UniProtKB/Swiss-Prot Q18581 (ACN1_CAEEL)

Names and origin

Protein names

Recommended name:
    Inactive angiotensin-converting enzyme-related protein
Alternative name(s):
    ACE-like non-metallopeptidase protein 1

Gene names

Name:	acn-1
ORF Names:	C42D8.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	906 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for larval molting, male tail development, and formation of adult alae. Inactive as a metallopeptidase, due to a lack of active site residues. Ref.2
Tissue specificity	Expressed in embryonic and larval hypodermis, in the vulva during organogenesis, and in the ray papillae of the male tail. Ref.2
Sequence similarities	Belongs to the peptidase M2 family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Signal
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Ref.2 Inferred from mutant phenotype. Source: WormBase locomotion Ref.2 Inferred from mutant phenotype. Source: WormBase molting cycle, collagen and cuticulin-based cuticle Ref.2 Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase oviposition Ref.2 Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Ref.2 Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Ref.2 Inferred from mutant phenotype. Source: WormBase proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: InterPro peptidyl-dipeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q18581-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q18581-2)

The sequence of this isoform differs from the canonical sequence as follows:
     160-197: SSNYWKTDNL...YEAEAIKVLR → LTLNHFSSTT...TEKELHSLCS
     198-773: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 906	887	Inactive angiotensin-converting enzyme-related protein		PRO_0000028567
Regions
	Compositional bias	24 – 93	70	Pro-rich
Amino acid modifications
	Glycosylation	159	1	N-linked (GlcNAc...) Ref.3 Ref.4
	Glycosylation	653	1	N-linked (GlcNAc...) Ref.4
Natural variations
	Alternative sequence	160 – 197	38	SSNYW…IKVLR → LTLNHFSSTTSHSSTGSAIP MKSIRLLLDGTEKELHSLCS in isoform b.		VSP_015490
	Alternative sequence	198 – 773	576	Missing in isoform b.		VSP_015491

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 50B3CD37EA62CBD3	FASTA	906	100,725
	10 20 30 40 50 60 MKFHILLLLL VGACLPVFTQ EIKPKPELLP ADEAPKDPEA VFSEGEPFEL TDALDTPKNG 70 80 90 100 110 120 SVPVPEPEPK PEPEPEPEPK PEPEPSPTPE PEPAIKFDNI ESEDYGDVAE TAASTQPDEL 130 140 150 160 170 180 NTEVIEQLVD TFLNTGSIAS NKTNKGPVFA NPVAQALVNS SNYWKTDNLQ APGSIKDEEK 190 200 210 220 230 240 LRSWLAGYEA EAIKVLREVA LSGWRYFNDA SPSLKLALDE AENVLTMFVR STSMQAKQFD 250 260 270 280 290 300 MASVTDEKVM RQLGYVSFEG MSALAPSRFA DYSQAQAALN RDSKDSTICD KDVPPPCALQ 310 320 330 340 350 360 KIDMDSIFRN EKDASRLQHL WVSYVTAIAK SKPSYNNIIT ISNEGAKLNG FANGGAMWRS 370 380 390 400 410 420 AFDMSSKVHK AEFDLNKQID KIYSTIQPFY QLLHAYMRRQ LAGIYSNPVG LSKDGPIPAH 430 440 450 460 470 480 LFGSLDGGDW SAHYEQTKPF EEESETPEAM LSAFNTQNYT TKKMFVTAYR YFKSAGFPHL 490 500 510 520 530 540 PKSYWTSSIF ARVWSKDMIC HPAAALDMRA PNDFRVKACA QLGEPDFEQA HSLLVQTYYQ 550 560 570 580 590 600 YLYKDQSLLF REQASPVITD AIANAFAHLS TNPHYLYSQK LVPSEHLDIK DSVIINKLYK 610 620 630 640 650 660 ESLESFTKLP FTIAADNWRY ELFDGTVPKN KLNDRWWEIR NKYEGVRSPQ PYNTSNLDAL 670 680 690 700 710 720 IHNSVSQVHS PATRTLISYV LKFQILKALC PEGTILSEGC ILSEDTTEKL RETMKLGSSI 730 740 750 760 770 780 TWLKALEMIS GKGELDAQPL LEYYEPLINW LRNTNEIDQV VVGWDGEGTP FTVEEIPKTR 790 800 810 820 830 840 QPGDGGNGLP SEDRVAFPGG ECVNGQECLL DSHCNGTICV CNDGLYTLEI GNTFNCVPGN 850 860 870 880 890 900 PADSGFGDGK GGLVIGLFNN EVTTPEPSAE PEPTAKTTTK MPPRVRAATS PFSLYLTVLL IIYFAL
	Isoform b. Checksum: DF2528B20E9D6CF6	FASTA	332	35,399
	10 20 30 40 50 60 MKFHILLLLL VGACLPVFTQ EIKPKPELLP ADEAPKDPEA VFSEGEPFEL TDALDTPKNG 70 80 90 100 110 120 SVPVPEPEPK PEPEPEPEPK PEPEPSPTPE PEPAIKFDNI ESEDYGDVAE TAASTQPDEL 130 140 150 160 170 180 NTEVIEQLVD TFLNTGSIAS NKTNKGPVFA NPVAQALVNL TLNHFSSTTS HSSTGSAIPM 190 200 210 220 230 240 KSIRLLLDGT EKELHSLCSE EIPKTRQPGD GGNGLPSEDR VAFPGGECVN GQECLLDSHC 250 260 270 280 290 300 NGTICVCNDG LYTLEIGNTF NCVPGNPADS GFGDGKGGLV IGLFNNEVTT PEPSAEPEPT 310 320 330 AKTTTKMPPR VRAATSPFSL YLTVLLIIYF AL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensin-converting enzyme family that lacks a metallopeptidase active site." Brooks D.R., Appleford P.J., Murray L., Isaac R.E. J. Biol. Chem. 278:52340-52346(2003) [PubMed: 14559923] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[3]	"Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins." Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T. Nat. Biotechnol. 21:667-672(2003) [PubMed: 12754521] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-159, MASS SPECTROMETRY.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-159 AND ASN-653, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U56966 Genomic DNA. Translation: AAU05584.1. U56966 Genomic DNA. Translation: AAA98719.2.
PIR	T15792.
RefSeq	NP_001024453.1. NP_001024454.1.
UniGene	Cel.7717
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q18581.
Proteomic databases
PRIDE	Q18581.
Genome annotation databases
Ensembl	C42D8.5a; C42D8.5a; C42D8.5; Caenorhabditis elegans. [Genome view]
GeneID	180780.
KEGG	cel:C42D8.5.
UCSC	C42D8.5b.1. c. elegans.
Organism-specific databases
CTD	180780.
WormBase	WBGene00000039. acn-1.
WormPep	C42D8.5a. CE30627. [WorfDB] C42D8.5b. CE37212. [WorfDB]
Phylogenomic databases
HOGENOM	HBG388110.
InParanoid	Q18581.
OMA	KNMDVRP.
Gene expression databases
ArrayExpress	Q18581.
Family and domain databases
InterPro	IPR001548. Peptidase_M2. [Graphical view]
PANTHER	PTHR10514. Peptidase_M2. 1 hit.
Pfam	PF01401. Peptidase_M2. 1 hit. [Graphical view]
PRINTS	PR00791. PEPDIPTASEA.
ProtoNet	Search...
Other Resources
NextBio	910922.

Entry information

Entry name

ACN1_CAEEL

Accession

Primary (citable) accession number: Q18581
Secondary accession number(s): Q65ZH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 1, 2002
Last modified:	December 15, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project