Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc metalloproteinase nas-8

Gene

nas-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Metalloprotease.By similarity

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi203Zinc; catalyticPROSITE-ProRule annotation1
Active sitei204PROSITE-ProRule annotation1
Metal bindingi207Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi213Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM12.A21

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-8 (EC:3.4.24.21By similarity)
Alternative name(s):
Nematode astacin 8
Gene namesi
Name:nas-8
ORF Names:C34D4.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC34D4.9 ; CE31327 ; WBGene00003527 ; nas-8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000044226530 – 111By similarityAdd BLAST82
ChainiPRO_0000028913112 – 403Zinc metalloproteinase nas-8Add BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi338 ↔ 372PROSITE-ProRule annotation
Disulfide bondi345 ↔ 365PROSITE-ProRule annotation
Disulfide bondi352 ↔ 369PROSITE-ProRule annotation
Glycosylationi386N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ18439

Expressioni

Gene expression databases

BgeeiWBGene00003527

Interactioni

Protein-protein interaction databases

STRINGi6239.C34D4.9

Structurei

3D structure databases

ProteinModelPortaliQ18439
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini338 – 372ShKTPROSITE-ProRule annotationAdd BLAST35

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3714 Eukaryota
ENOG410ZPX7 LUCA
GeneTreeiENSGT00760000119227
HOGENOMiHOG000018063
InParanoidiQ18439
KOiK08076
OMAiQAYHDKT
OrthoDBiEOG091G009U
PhylomeDBiQ18439

Family and domain databases

CDDicd04280 ZnMc_astacin_like, 1 hit
Gene3Di3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR034035 Astacin-like_dom
IPR017367 Caenorhab_nas-7/8
IPR024079 MetalloPept_cat_dom_sf
IPR001506 Peptidase_M12A
IPR006026 Peptidase_Metallo
IPR003582 ShKT_dom
PfamiView protein in Pfam
PF01400 Astacin, 1 hit
PF01549 ShK, 1 hit
PIRSFiPIRSF038054 Nas7/Nas8_prd, 1 hit
PRINTSiPR00480 ASTACIN
SMARTiView protein in SMART
SM00254 ShKT, 1 hit
SM00235 ZnMc, 1 hit
PROSITEiView protein in PROSITE
PS51670 SHKT, 1 hit
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q18439-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRNDLLNNK ITIFLSSLSL FVIIIPIYAA EKDLLPPSTS SETFLTDEDF
60 70 80 90 100
LRPLNDDETF LTEKDFKNGE KLGEDHVPAG SILWKQVYKK GDIRGKAAWK
110 120 130 140 150
LDPKNSESLR RNGVITGTRK WPNGRIPYVI SNQYNDRERA VLARSFQAYH
160 170 180 190 200
DKTCVRFVPR TAVDNDYLYI GKIDGCYSDV GRAGGRQELS LDNGCLQYDT
210 220 230 240 250
AIHELMHSVG FYHEHERWDR DEHITILWHN IDREAYDQFG KVDLAESSYY
260 270 280 290 300
GQLYDYYSIM HYDSLAFSKN GFETMVAKQS EMTAVIGAAI DFSPIDILKM
310 320 330 340 350
NLMYQCSDVK LPPTVVGTTD KTIVPVPAPS VTVVEDDCRD RTNLCWRWID
360 370 380 390 400
RCKSFFFEQI MKEFCSLSCG YCTPKALQTA KASPPNYSNT LLTKSSTSYL

QHG
Length:403
Mass (Da):46,095
Last modified:October 1, 2002 - v3
Checksum:iF1C02C8CE1428981
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080770 Genomic DNA Translation: CCD66603.1
AJ561204 mRNA Translation: CAD99207.1
PIRiT29277
RefSeqiNP_501114.2, NM_068713.6
UniGeneiCel.20243

Genome annotation databases

EnsemblMetazoaiC34D4.9; C34D4.9; WBGene00003527
GeneIDi183207
KEGGicel:CELE_C34D4.9
UCSCiC34D4.9 c. elegans

Similar proteinsi

Entry informationi

Entry nameiNAS8_CAEEL
AccessioniPrimary (citable) accession number: Q18439
Secondary accession number(s): Q7Z0N4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 115 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health