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Protein

Zinc metalloproteinase nas-8

Gene

nas-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable metalloprotease.By similarity

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi203 – 2031Zinc; catalyticPROSITE-ProRule annotation
Active sitei204 – 2041PROSITE-ProRule annotation
Metal bindingi207 – 2071Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi213 – 2131Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.A21.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-8 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 8
Gene namesi
Name:nas-8
ORF Names:C34D4.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC34D4.9; CE31327; WBGene00003527; nas-8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 403374Zinc metalloproteinase nas-8PRO_0000028913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi338 ↔ 372PROSITE-ProRule annotation
Disulfide bondi345 ↔ 365PROSITE-ProRule annotation
Disulfide bondi352 ↔ 369PROSITE-ProRule annotation
Glycosylationi386 – 3861N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ18439.

Interactioni

Protein-protein interaction databases

STRINGi6239.C34D4.9.

Structurei

3D structure databases

ProteinModelPortaliQ18439.
SMRiQ18439. Positions 121-306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini338 – 37235ShKTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000018063.
InParanoidiQ18439.
KOiK08076.
OMAiQAYHDKT.
OrthoDBiEOG73V6MT.
PhylomeDBiQ18439.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR017367. Caenorhab_nas-7/8.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PIRSFiPIRSF038054. Nas7/Nas8_prd. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00254. ShKT. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS51670. SHKT. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q18439-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRNDLLNNK ITIFLSSLSL FVIIIPIYAA EKDLLPPSTS SETFLTDEDF
60 70 80 90 100
LRPLNDDETF LTEKDFKNGE KLGEDHVPAG SILWKQVYKK GDIRGKAAWK
110 120 130 140 150
LDPKNSESLR RNGVITGTRK WPNGRIPYVI SNQYNDRERA VLARSFQAYH
160 170 180 190 200
DKTCVRFVPR TAVDNDYLYI GKIDGCYSDV GRAGGRQELS LDNGCLQYDT
210 220 230 240 250
AIHELMHSVG FYHEHERWDR DEHITILWHN IDREAYDQFG KVDLAESSYY
260 270 280 290 300
GQLYDYYSIM HYDSLAFSKN GFETMVAKQS EMTAVIGAAI DFSPIDILKM
310 320 330 340 350
NLMYQCSDVK LPPTVVGTTD KTIVPVPAPS VTVVEDDCRD RTNLCWRWID
360 370 380 390 400
RCKSFFFEQI MKEFCSLSCG YCTPKALQTA KASPPNYSNT LLTKSSTSYL

QHG
Length:403
Mass (Da):46,095
Last modified:October 1, 2002 - v3
Checksum:iF1C02C8CE1428981
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080770 Genomic DNA. Translation: CCD66603.1.
AJ561204 mRNA. Translation: CAD99207.1.
PIRiT29277.
RefSeqiNP_501114.2. NM_068713.6.
UniGeneiCel.20243.

Genome annotation databases

EnsemblMetazoaiC34D4.9; C34D4.9; WBGene00003527.
GeneIDi183207.
KEGGicel:CELE_C34D4.9.
UCSCiC34D4.9. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080770 Genomic DNA. Translation: CCD66603.1.
AJ561204 mRNA. Translation: CAD99207.1.
PIRiT29277.
RefSeqiNP_501114.2. NM_068713.6.
UniGeneiCel.20243.

3D structure databases

ProteinModelPortaliQ18439.
SMRiQ18439. Positions 121-306.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C34D4.9.

Protein family/group databases

MEROPSiM12.A21.

Proteomic databases

PaxDbiQ18439.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC34D4.9; C34D4.9; WBGene00003527.
GeneIDi183207.
KEGGicel:CELE_C34D4.9.
UCSCiC34D4.9. c. elegans.

Organism-specific databases

CTDi183207.
WormBaseiC34D4.9; CE31327; WBGene00003527; nas-8.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000018063.
InParanoidiQ18439.
KOiK08076.
OMAiQAYHDKT.
OrthoDBiEOG73V6MT.
PhylomeDBiQ18439.

Miscellaneous databases

NextBioi920302.
PROiQ18439.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR017367. Caenorhab_nas-7/8.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PIRSFiPIRSF038054. Nas7/Nas8_prd. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00254. ShKT. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS51670. SHKT. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "The astacin protein family in Caenorhabditis elegans."
    Moehrlen F., Hutter H., Zwilling R.
    Eur. J. Biochem. 270:4909-4920(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 159-195, NOMENCLATURE.
    Strain: Bristol N2.

Entry informationi

Entry nameiNAS8_CAEEL
AccessioniPrimary (citable) accession number: Q18439
Secondary accession number(s): Q7Z0N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2002
Last modified: November 11, 2015
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.