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Protein

Guanine nucleotide-binding protein alpha-17 subunit

Gene

odr-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:10192394). This specific G-alpha subunit plays an important role in olfaction and in cilia morphogenesis (PubMed:9459442, PubMed:15342507). Involved in chemotactic responses to attractants diacetyl, pyrazine, 2,4,5-trimethylthiazole, benzaldehyde, isoamyl alcohol, butanone and 2,3-pentanedione (PubMed:12694294). Displays a redundant function with gpa-3 in chemotactic responses (PubMed:12694294). Involved in avoidance responses to copper, sodium dodecyl sulfate and linoleic acid (PubMed:12694294). Involved in osmotic avoidance and mechanosensory responses (PubMed:12694294). Involved in specifying fan-like morphology of cilia of head sensory neurons AWC (PubMed:9459442). Plays a role in the detection of preferred food sources by mediating the recognition of food oders in olfactory sensory neurons (PubMed:25009271).5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471MagnesiumBy similarity
Metal bindingi183 – 1831MagnesiumBy similarity
Binding sitei328 – 3281GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 478GTPBy similarity
Nucleotide bindingi177 – 1837GTPBy similarity
Nucleotide bindingi202 – 2065GTPBy similarity
Nucleotide bindingi271 – 2744GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • olfactory receptor binding Source: WormBase
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: InterPro
  • chemotaxis Source: UniProtKB-KW
  • cilium morphogenesis Source: UniProtKB
  • hyperosmotic response Source: WormBase
  • olfactory behavior Source: UniProtKB
  • response to odorant Source: UniProtKB
  • sensory perception of smell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Chemotaxis, Olfaction, Sensory transduction

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein alpha-17 subunit
Alternative name(s):
Odorant response abnormal protein 3
Gene namesi
Name:odr-3
ORF Names:C34D1.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC34D1.3; CE08571; WBGene00003850; odr-3.

Subcellular locationi

  • Cell projectioncilium 1 Publication
  • Cell projectiondendrite 1 Publication

  • Note: In amphid neurons also weakly expressed in cell body. In phasmid neurons found only in cilia.

GO - Cellular componenti

  • dendrite Source: UniProtKB-SubCell
  • neuronal cell body Source: WormBase
  • nonmotile primary cilium Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium

Pathology & Biotechi

Disruption phenotypei

Defective in chemotactic responses to attractants and repellents and in osmotic and mechanosensory avoidance.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471S → C: Loss of chemotactic responses. 1 Publication
Mutagenesisi206 – 2061Q → L: Loss of chemotactic and osmotic avoidance responses. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 356355Guanine nucleotide-binding protein alpha-17 subunitPRO_0000203659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Lipidationi4 – 41S-palmitoyl cysteineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiQ18434.
PRIDEiQ18434.

Expressioni

Tissue specificityi

Expressed in sensory neurons in the head and tail. Expressed in amphid AWC neurons, to a lesser extent in AWB and weakly in AWA, ASH and ADF neurons (head sensory neurons). Expressed in phasmid PHA and PHB neurons (tail sensory neurons).2 Publications

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (By similarity).By similarity

GO - Molecular functioni

  • olfactory receptor binding Source: WormBase

Protein-protein interaction databases

STRINGi6239.C34D1.3.

Structurei

3D structure databases

ProteinModelPortaliQ18434.
SMRiQ18434. Positions 16-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
InParanoidiQ18434.
KOiK04640.
OMAiMRIHEPG.
OrthoDBiEOG75XGQN.
PhylomeDBiQ18434.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q18434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCQSNENS EGNARNKEIE KQLNADKRAG SSIVKLLLLG AGECGKSTVL
60 70 80 90 100
KQMQILHSNG FTEEEVNEKR AIVYNNTVSA MCTILRAMDG VLHLPLENGQ
110 120 130 140 150
KEAEKAIVMK VQENGEEGEA LTEEVSKAIQ SLWADPGVKK AFEMRSEYQL
160 170 180 190 200
PDSAKYFLDN CQRISEPGYR PNDQDILYSR VATTGVVEVK FKIKELDFRV
210 220 230 240 250
FDVGGQRSER RKWIHCFDNV ESIIFITAIS EYDQVLFEDE TTNRMIESMQ
260 270 280 290 300
LFNSICNSTW FLSTAMILFM NKKDLFMEKI QRVNITTAFP DYEGGQNYEE
310 320 330 340 350
AVSFIKQKFA ELNLNPDKKT IYMHETCATD TNQVQLVISS VIDTIIQKNL

QKAGMM
Length:356
Mass (Da):40,434
Last modified:November 1, 1996 - v1
Checksum:iCB9F36F5F4FB95D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008142 mRNA. Translation: AAG32095.1.
FJ455729 Genomic DNA. Translation: ACQ43989.1.
FJ455730 Genomic DNA. Translation: ACQ43990.1.
FJ455731 Genomic DNA. Translation: ACQ43991.1.
Z78060 Genomic DNA. Translation: CAB01489.1.
AY146558 Genomic DNA. Translation: AAN78230.1.
AY146559 Genomic DNA. Translation: AAN78231.1.
AY146560 Genomic DNA. Translation: AAN78232.1.
AY146561 Genomic DNA. Translation: AAN78233.1.
AY146562 Genomic DNA. Translation: AAN78234.1.
AY146563 Genomic DNA. Translation: AAN78235.1.
AY146564 Genomic DNA. Translation: AAN78236.1.
AY146565 Genomic DNA. Translation: AAN78237.1.
AY146566 Genomic DNA. Translation: AAN78238.1.
PIRiT19715.
RefSeqiNP_506290.1. NM_073889.1.
UniGeneiCel.2932.

Genome annotation databases

EnsemblMetazoaiC34D1.3; C34D1.3; WBGene00003850.
GeneIDi179806.
KEGGicel:CELE_C34D1.3.
UCSCiC34D1.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008142 mRNA. Translation: AAG32095.1.
FJ455729 Genomic DNA. Translation: ACQ43989.1.
FJ455730 Genomic DNA. Translation: ACQ43990.1.
FJ455731 Genomic DNA. Translation: ACQ43991.1.
Z78060 Genomic DNA. Translation: CAB01489.1.
AY146558 Genomic DNA. Translation: AAN78230.1.
AY146559 Genomic DNA. Translation: AAN78231.1.
AY146560 Genomic DNA. Translation: AAN78232.1.
AY146561 Genomic DNA. Translation: AAN78233.1.
AY146562 Genomic DNA. Translation: AAN78234.1.
AY146563 Genomic DNA. Translation: AAN78235.1.
AY146564 Genomic DNA. Translation: AAN78236.1.
AY146565 Genomic DNA. Translation: AAN78237.1.
AY146566 Genomic DNA. Translation: AAN78238.1.
PIRiT19715.
RefSeqiNP_506290.1. NM_073889.1.
UniGeneiCel.2932.

3D structure databases

ProteinModelPortaliQ18434.
SMRiQ18434. Positions 16-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C34D1.3.

Proteomic databases

PaxDbiQ18434.
PRIDEiQ18434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC34D1.3; C34D1.3; WBGene00003850.
GeneIDi179806.
KEGGicel:CELE_C34D1.3.
UCSCiC34D1.3. c. elegans.

Organism-specific databases

CTDi179806.
WormBaseiC34D1.3; CE08571; WBGene00003850; odr-3.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
InParanoidiQ18434.
KOiK04640.
OMAiMRIHEPG.
OrthoDBiEOG75XGQN.
PhylomeDBiQ18434.

Miscellaneous databases

NextBioi906938.
PROiQ18434.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The G alpha protein ODR-3 mediates olfactory and nociceptive function and controls cilium morphogenesis in C. elegans olfactory neurons."
    Roayaie K., Crump J.G., Sagasti A., Bargmann C.I.
    Neuron 20:55-67(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF SER-47 AND GLN-206.
  2. "Proteins Interacting With Caenorhabditis elegans Galpha Subunits."
    Cuppen E., van der Linden A.M., Jansen G., Plasterk R.H.
    Comp. Funct. Genomics 4:479-491(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  3. "High nucleotide divergence in developmental regulatory genes contrasts with the structural elements of olfactory pathways in caenorhabditis."
    Jovelin R., Dunham J.P., Sung F.S., Phillips P.C.
    Genetics 181:1387-1397(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PX174, PX178 and PX179.
  4. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  5. "Molecular evolution and quantitative variation for chemosensory behaviour in the nematode genus Caenorhabditis."
    Jovelin R., Ajie B.C., Phillips P.C.
    Mol. Ecol. 12:1325-1337(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-350, FUNCTION, DISRUPTION PHENOTYPE.
    Strain: AB3, BO, CB4855, CB4856, CB4857, CB4932, DH424, RC301 and TR403.
  6. "The complete family of genes encoding G proteins of Caenorhabditis elegans."
    Jansen G., Thijssen K.L., Werner P., van der Horst M., Hazendonk E., Plasterk R.H.A.
    Nat. Genet. 21:414-419(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
  7. "A network of stimulatory and inhibitory Galpha-subunits regulates olfaction in Caenorhabditis elegans."
    Lans H., Rademakers S., Jansen G.
    Genetics 167:1677-1687(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  8. "Dissecting the signaling mechanisms underlying recognition and preference of food odors."
    Harris G., Shen Y., Ha H., Donato A., Wallis S., Zhang X., Zhang Y.
    J. Neurosci. 34:9389-9403(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGPA17_CAEEL
AccessioniPrimary (citable) accession number: Q18434
Secondary accession number(s): C3U544
, Q867E1, Q867N8, Q868B7, Q86FY4, Q86FY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.