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Protein

D-aminoacyl-tRNA deacylase

Gene

dtd

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.UniRule annotation

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).UniRule annotation
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-2901-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylaseUniRule annotation (EC:3.1.1.96UniRule annotation)
Short name:
DTDUniRule annotation
Alternative name(s):
Gly-tRNA(Ala) deacylaseUniRule annotation (EC:3.1.1.-UniRule annotation)
Gene namesi
Name:dtdUniRule annotation
Ordered Locus Names:CD630_27430
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002592711 – 149D-aminoacyl-tRNA deacylaseAdd BLAST149

Proteomic databases

PRIDEiQ183H9

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD2743

Structurei

3D structure databases

ProteinModelPortaliQ183H9
SMRiQ183H9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 138Gly-cisPro motif, important for rejection of L-amino acidsUniRule annotation2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.UniRule annotation

Sequence similaritiesi

Belongs to the DTD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108YYA Bacteria
COG1490 LUCA
HOGENOMiHOG000113981
KOiK07560
OMAiGVFQAHM

Family and domain databases

CDDicd00563 Dtyr_deacylase, 1 hit
Gene3Di3.50.80.10, 1 hit
HAMAPiMF_00518 Deacylase_Dtd, 1 hit
InterProiView protein in InterPro
IPR003732 Daa-tRNA_deacyls_DTD
IPR023509 DTD-like_sf
PANTHERiPTHR10472 PTHR10472, 1 hit
PfamiView protein in Pfam
PF02580 Tyr_Deacylase, 1 hit
SUPFAMiSSF69500 SSF69500, 1 hit
TIGRFAMsiTIGR00256 TIGR00256, 1 hit

Sequencei

Sequence statusi: Complete.

Q183H9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVVQRVSS SKVTVDENTI GQINKGLLVL LGVTHDDKSS DVDYMIDKIL
60 70 80 90 100
NLRIFEDEND KMNLSLMDIG GELLVVSQFT LYGDCRKGRR PGFSNAARPE
110 120 130 140
LANNLYEEFV KKAKDKGVTV GTGQFAAHMM VELTNDGPVT ILLDSSKSF
Length:149
Mass (Da):16,457
Last modified:July 25, 2006 - v1
Checksum:i6733FCFCEBAF452D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ69630.1
RefSeqiWP_003426448.1, NZ_CP010905.2
YP_001089255.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ69630; CAJ69630; CD630_27430
GeneIDi31354344
4915428
KEGGicdf:CD630_27430
pdc:CDIF630_03006
PATRICifig|272563.120.peg.2890

Entry informationi

Entry nameiDTD_PEPD6
AccessioniPrimary (citable) accession number: Q183H9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 25, 2006
Last modified: May 23, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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