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Q183G0 (GLYA_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:CD630_27260
OrganismClostridium difficile (strain 630) [Complete proteome] [HAMAP]
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_0000369912

Regions

Region122 – 1243Substrate binding By similarity

Sites

Binding site321Pyridoxal phosphate By similarity
Binding site521Pyridoxal phosphate By similarity
Binding site541Substrate By similarity
Binding site611Substrate binding By similarity
Binding site621Pyridoxal phosphate By similarity
Binding site1181Substrate By similarity
Binding site1731Pyridoxal phosphate By similarity
Binding site2011Pyridoxal phosphate By similarity
Binding site2261Pyridoxal phosphate By similarity
Binding site2331Pyridoxal phosphate By similarity
Binding site2581Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3581Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2271N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q183G0 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: F8ED8972FE4E77EF

FASTA41445,795
        10         20         30         40         50         60 
MFENLKKADP EIYESMKREL KRQQRNIELI ASENIVSVPV METMGSHLTN KYAEGYSGKR 

        70         80         90        100        110        120 
YYGGCEYIDE VETLAIDRIK KIFGAEHANV QPHSGANANI GVYFAMLKPG DVVMGMNLSQ 

       130        140        150        160        170        180 
GGHLTHGAPV NISGQYYKFY EYGIDKDSGL IDFDEVRKIA HEVKPKMIVA GASAYPREID 

       190        200        210        220        230        240 
FKKFREIADE VGALLMVDMA HIAGLVAAGL HQNPCEVADF VTTTTHKTLR GPRGGVILCK 

       250        260        270        280        290        300 
EKYAKDIDKA IFPGIQGGPL EHIIASKAVC FKEALSDEFK EYQVQVAKNA KALAEELIKR 

       310        320        330        340        350        360 
DFKLISGGTD NHLILLDLTN KNITGKAAEK RLDDAYITAN KNTIPFDPNG ALVTSGIRLG 

       370        380        390        400        410 
TPAVTTRGMK EEDMAIIAEA IDLCLTYDEE SKARTLVVGL TEKYPLYEEY NVMD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM180355 Genomic DNA. Translation: CAJ69613.1.
RefSeqYP_001089238.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ183G0.
SMRQ183G0. Positions 5-402.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ183G0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4912877.
GenomeReviewsGene locus CD2726 in contig AM180355_GR.
KEGGcdf:CD2726.
NMPDRfig|1496.1.peg.2308.
PATRIC19443939. VBICloDif38397_2853.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0112.
HOGENOMHBG301263.
OMAKAICAKF.
ProtClustDBCLSK2535392.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_CLOD6
AccessionPrimary (citable) accession number: Q183G0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 25, 2006
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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