Q183C9 (Q183C9_CLOD6) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase HAMAP MF_00555
EC=6.3.1.1 HAMAP MF_00555

Alternative name(s):
Asparagine synthetase A HAMAP MF_00555

Gene names

Name:	asnA HAMAP MF_00555 EMBL CAJ69581.2
Ordered Locus Names:	CD630_26950

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555 SAAS SAAS004618
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555 SAAS SAAS004618
Subcellular location	Cytoplasm By similarity HAMAP MF_00555 SAAS SAAS004618.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. HAMAP MF_00555

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis HAMAP MF_00555 SAAS SAAS004618
Cellular component	Cytoplasm HAMAP MF_00555 SAAS SAAS004618
Ligand	ATP-binding HAMAP MF_00555 SAAS SAAS004618 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00555 SAAS SAAS004618 EMBL CAJ69581.2
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q183C9 [UniParc]. Last modified June 28, 2011. Version 2. Checksum: 7322E791E896C335	FASTA	333	38,148
	10 20 30 40 50 60 MSTIIPKDYK SSLNVIDTQI AIKKLKDFFE MRLSNELNLI RVSSPLFVLP ETGANDNLNG 70 80 90 100 110 120 EKAVSFDIPF MNKNAEIVQS LAKWKRLALK KYGFEVGSGL YTDMNAIRKN EELDNIHSLY 130 140 150 160 170 180 VDQWDWELVI DKSSRNEKTL KDVVKRLYGV FKDTEIFVCS MYEGIKEILP EEITFITSQE 190 200 210 220 230 240 LEDMYPELSP KEREDKIVKE KKAVFITQIG KTLISGEKHD GRSPDYDDWE LNGDILFYNP 250 260 270 280 290 300 VLDSALELSS MGIRVDEESL DRQLKLAGCE ERKEFDYHKM LLNGELPYTI GGGIGQSRIC 310 320 330 MYFLQKAHIG EVQVGVWPQD MIQNCSSAGI ELL

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 630.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180355 Genomic DNA. Translation: CAJ69581.2.
RefSeq	YP_001089206.1. NC_009089.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q183C9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4914899.
GenomeReviews	Gene locus CD2695 in contig AM180355_GR.
KEGG	cdf:CD2695.
PATRIC	19443867. VBICloDif38397_2820.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LNDNLNG.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q183C9_CLOD6

Accession

Primary (citable) accession number: Q183C9

Entry history

Integrated into UniProtKB/TrEMBL:	July 25, 2006
Last sequence update:	June 28, 2011
Last modified:	January 25, 2012
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)