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Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.UniRule annotation

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.UniRule annotation

Pathwayi: lipoprotein biosynthesis (signal peptide cleavage)

This protein is involved in the pathway lipoprotein biosynthesis (signal peptide cleavage), which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipoprotein biosynthesis (signal peptide cleavage) and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101UniRule annotation1
Active sitei126UniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-2754-MONOMER.
UniPathwayiUPA00665.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidaseUniRule annotation (EC:3.4.23.36UniRule annotation)
Alternative name(s):
Prolipoprotein signal peptidaseUniRule annotation
Signal peptidase IIUniRule annotation
Short name:
SPase IIUniRule annotation
Gene namesi
Name:lspAUniRule annotation
Ordered Locus Names:CD630_25970
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 43HelicalUniRule annotationAdd BLAST23
Transmembranei57 – 77HelicalUniRule annotationAdd BLAST21
Transmembranei80 – 100HelicalUniRule annotationAdd BLAST21
Transmembranei124 – 144HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002893681 – 148Lipoprotein signal peptidaseAdd BLAST148

Interactioni

Protein-protein interaction databases

STRINGi272563.CD2597.

Structurei

3D structure databases

ProteinModelPortaliQ182T8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
HOGENOMiHOG000096992.
KOiK03101.
OMAiASEIHGT.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q182T8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYILIIILL IGLDQLSKIW VLNNLVDVST IPIINNVFHL TYVENRGAAF
60 70 80 90 100
GLLQNNQWIF IIVALLATVF GLYYLNTRKV HIFGRLGIIL IISGALGNLI
110 120 130 140
DRVRLGFVVD YFDFRIIWEY VFNIADVFVV VGTVFLCIYV LFFESKSR
Length:148
Mass (Da):17,020
Last modified:July 25, 2006 - v1
Checksum:i764CA7E7EA649688
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ69487.1.
RefSeqiWP_004454916.1. NZ_CP010905.1.
YP_001089114.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ69487; CAJ69487; CD630_25970.
GeneIDi4915120.
KEGGicdf:CD630_25970.
pdc:CDIF630_02851.
PATRICi19443671. VBICloDif38397_2722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ69487.1.
RefSeqiWP_004454916.1. NZ_CP010905.1.
YP_001089114.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ182T8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD2597.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ69487; CAJ69487; CD630_25970.
GeneIDi4915120.
KEGGicdf:CD630_25970.
pdc:CDIF630_02851.
PATRICi19443671. VBICloDif38397_2722.

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
HOGENOMiHOG000096992.
KOiK03101.
OMAiASEIHGT.

Enzyme and pathway databases

UniPathwayiUPA00665.
BioCyciPDIF272563:G12WB-2754-MONOMER.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSPA_PEPD6
AccessioniPrimary (citable) accession number: Q182T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.