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Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.UniRule annotation

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.UniRule annotation

Pathwayi: lipoprotein biosynthesis (signal peptide cleavage)

This protein is involved in the pathway lipoprotein biosynthesis (signal peptide cleavage), which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipoprotein biosynthesis (signal peptide cleavage) and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101UniRule annotation1
Active sitei126UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-2754-MONOMER
UniPathwayiUPA00665

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidaseUniRule annotation (EC:3.4.23.36UniRule annotation)
Alternative name(s):
Prolipoprotein signal peptidaseUniRule annotation
Signal peptidase IIUniRule annotation
Short name:
SPase IIUniRule annotation
Gene namesi
Name:lspAUniRule annotation
Ordered Locus Names:CD630_25970
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 43HelicalUniRule annotationAdd BLAST23
Transmembranei57 – 77HelicalUniRule annotationAdd BLAST21
Transmembranei80 – 100HelicalUniRule annotationAdd BLAST21
Transmembranei124 – 144HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002893681 – 148Lipoprotein signal peptidaseAdd BLAST148

Interactioni

Protein-protein interaction databases

STRINGi272563.CD2597

Structurei

3D structure databases

ProteinModelPortaliQ182T8
SMRiQ182T8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105M02 Bacteria
COG0597 LUCA
HOGENOMiHOG000096992
KOiK03101
OMAiFGHVVDY

Family and domain databases

HAMAPiMF_00161 LspA, 1 hit
InterProiView protein in InterPro
IPR001872 Peptidase_A8
PANTHERiPTHR33695 PTHR33695, 1 hit
PfamiView protein in Pfam
PF01252 Peptidase_A8, 1 hit
PRINTSiPR00781 LIPOSIGPTASE
TIGRFAMsiTIGR00077 lspA, 1 hit
PROSITEiView protein in PROSITE
PS00855 SPASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q182T8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYILIIILL IGLDQLSKIW VLNNLVDVST IPIINNVFHL TYVENRGAAF
60 70 80 90 100
GLLQNNQWIF IIVALLATVF GLYYLNTRKV HIFGRLGIIL IISGALGNLI
110 120 130 140
DRVRLGFVVD YFDFRIIWEY VFNIADVFVV VGTVFLCIYV LFFESKSR
Length:148
Mass (Da):17,020
Last modified:July 25, 2006 - v1
Checksum:i764CA7E7EA649688
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ69487.1
RefSeqiWP_004454916.1, NZ_CP010905.2
YP_001089114.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ69487; CAJ69487; CD630_25970
GeneIDi31354195
4915120
KEGGicdf:CD630_25970
pdc:CDIF630_02851
PATRICifig|272563.120.peg.2739

Similar proteinsi

Entry informationi

Entry nameiLSPA_PEPD6
AccessioniPrimary (citable) accession number: Q182T8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 25, 2006
Last modified: March 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health