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Protein

Ribonuclease Z

Gene

rnz

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Zinc 1; catalyticUniRule annotation1
Metal bindingi63Zinc 1; catalyticUniRule annotation1
Active sitei65Proton acceptorUniRule annotation1
Metal bindingi65Zinc 2; catalyticUniRule annotation1
Metal bindingi66Zinc 2; catalyticUniRule annotation1
Metal bindingi141Zinc 1; catalyticUniRule annotation1
Metal bindingi209Zinc 1; catalyticUniRule annotation1
Metal bindingi209Zinc 2; catalyticUniRule annotation1
Metal bindingi268Zinc 2; catalyticUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-2694-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease ZUniRule annotation (EC:3.1.26.11UniRule annotation)
Short name:
RNase ZUniRule annotation
Alternative name(s):
tRNA 3 endonucleaseUniRule annotation
tRNase ZUniRule annotation
Gene namesi
Name:rnzUniRule annotation
Ordered Locus Names:CD630_25390
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000702701 – 305Ribonuclease ZAdd BLAST305

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD2539.

Structurei

3D structure databases

ProteinModelPortaliQ182M7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase Z family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105IES. Bacteria.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

Q182M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDLTLLGCG GNVPMPNRFL SSVFINYKGR KILIDCGEGT QVSMKLKKCG
60 70 80 90 100
FKDIDLICIT HLHGDHIFGL LGLLSTIGNS GRTSDLTIVG PVGIVDCIRS
110 120 130 140 150
MRNLVEYVPY TLKIIENPQG NFSLDNKVLR NLEISTISLE HSIECLGYSF
160 170 180 190 200
NFRRNPKFDI DKATKNEVPK ILWNKLQEGQ NIVLDSKQYT PDMVLGELRK
210 220 230 240 250
GVKISLTTDT RPIESIPDFI KDSDLFICEA MYGDDLDISK AVRNKHMTFR
260 270 280 290 300
EAANLAKLGN VKQLLLTHFS PSLDIPSMYL ENATNVFENT ILGEDRLSLR

LNFDE
Length:305
Mass (Da):34,276
Last modified:July 25, 2006 - v1
Checksum:i0CFF474B0A34E432
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ69426.1.
RefSeqiWP_004454848.1. NZ_CP010905.1.
YP_001089053.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ69426; CAJ69426; CD630_25390.
GeneIDi4913436.
KEGGicdf:CD630_25390.
pdc:CDIF630_02791.
PATRICi19443551. VBICloDif38397_2662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ69426.1.
RefSeqiWP_004454848.1. NZ_CP010905.1.
YP_001089053.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ182M7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD2539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ69426; CAJ69426; CD630_25390.
GeneIDi4913436.
KEGGicdf:CD630_25390.
pdc:CDIF630_02791.
PATRICi19443551. VBICloDif38397_2662.

Phylogenomic databases

eggNOGiENOG4105IES. Bacteria.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-2694-MONOMER.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNZ_PEPD6
AccessioniPrimary (citable) accession number: Q182M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.