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Q182K8 (SYL_PEPD6) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CD630_25210
OrganismPeptoclostridium difficile (strain 630) (Clostridium difficile) [Complete proteome] [HAMAP]
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium

Protein attributes

Sequence length806 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 806806Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091308

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif566 – 5705"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5691ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q182K8 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 48AEB19A0A21102C

FASTA80692,433
        10         20         30         40         50         60 
MSVYNFKEVE SKWQKIWKDN NQYKMDTAQT EKPNYYTLEM FPYPSGKIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVARFKKMEG YNVLHPMGWD SFGLPAENAA IKHGIHPHKW TMENIEEMKE QLNLLGISYD 

       130        140        150        160        170        180 
WDKEVATSTP EYYRFTQEIF LKFLEHGLAY KKKSYVNWCP SCETVLANEQ VVQGACERCK 

       190        200        210        220        230        240 
ATVLKKDLEQ WYFKTTEFAE ELLNDLDTLD GWPEKVKTMQ KNWIGKSTGA DLVFDIDGTD 

       250        260        270        280        290        300 
KSMTVFTTRP DTTYGVTYMV LAPEHELVKE LVAGTEYEAD VEAFVQKMHT MTEIERTAAD 

       310        320        330        340        350        360 
VEKEGMFIGR YVINPLNGKK VPLWIANYVL VEYGTGAIMA VPAHDERDRD FAEKYNLDII 

       370        380        390        400        410        420 
DVITEDNKMI NSEEFNGLDA SEGFEGIIDK LEKEGRGKRT INYRLRDWLV SRQRYWGCPI 

       430        440        450        460        470        480 
PVVYCDECGI VPVKKEDLPV LLPTDVEFTG KGESPLTTSK QFMSTTCPHC GKPARREVDT 

       490        500        510        520        530        540 
MDTFVDSSWY FLRYVDSNNE NEPFSKELVN RWHPVDQYIG GVEHAIMHLL YARWFVKAFK 

       550        560        570        580        590        600 
SMGMVDFNEP FKNLLTQGMV LMDGSKMSKS KGNTVSPMDI IDEYGADTAR LFVLFAAPPE 

       610        620        630        640        650        660 
RDLDWSEQGV DGCFRFLNRV YRLVDELADV VKKDVEFGEL NSQDKDMRYT IHSTLKKVTA 

       670        680        690        700        710        720 
DLSEKFGFNT AISALMELIN DMYKYKELDN INEAVIKEGV QTIVTIIAPF APHLGEELWT 

       730        740        750        760        770        780 
MIGKEGSVFD IDWPKYDEKA LVKDEIEVVV QVNGKVRGKL TVNSNISKDE MEKVALEDEK 

       790        800 
IKGLVEGKTI VKVVAVPKKL VNIVVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM180355 Genomic DNA. Translation: CAJ69408.1.
RefSeqYP_001089035.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ182K8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272563.CD2521.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ69408; CAJ69408; CD630_25210.
GeneID4915278.
KEGGcdf:CD630_25210.
PATRIC19443515. VBICloDif38397_2644.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCDIF272563:GJFE-2740-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PEPD6
AccessionPrimary (citable) accession number: Q182K8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries