Q182B9 (SYGA_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	CD630_24330

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000047412

Sequences

	Sequence		Length	Mass (Da)
	Q182B9 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 66CF525737518BC9	FASTA	292	33,858
	10 20 30 40 50 60 MNFQEMILAL QKYWSKQGCI MMQPYDIEKG AGTMNPNTFL RSLGPEPWQV CYVEPSRRPA 70 80 90 100 110 120 DGRYGENPNR LYQHHQFQVI LKPSPDNIQE LYLESLKEIG IDPSEHDIRF VEDNWEAATV 130 140 150 160 170 180 GAWGLGWEVW LDGMEITQFT YFQQVGNIEC ELETGEITYG LERLAMYIQE VDSVYDLKWN 190 200 210 220 230 240 DKITYGEVFN KAEYENSMYA FELCDADMLF NLFDIYEKEA LRLMENGLVI PSYDYVLKCS 250 260 270 280 290 HAFNTLDARG AIGVSQRASF IGRVRNMAKT VAETFVKQRE EMGFPLLKDG DK

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 630.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180355 Genomic DNA. Translation: CAJ69319.1.
RefSeq	YP_001088946.1. NC_009089.1.
3D structure databases
ProteinModelPortal	Q182B9.
SMR	Q182B9. Positions 1-281.
ModBase	Search...
Protein-protein interaction databases
STRING	Q182B9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4915809.
GenomeReviews	Gene locus CD2433 in contig AM180355_GR.
KEGG	cdf:CD2433.
NMPDR	fig|1496.1.peg.2412.
PATRIC	19443331. VBICloDif38397_2552.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0752.
HOGENOM	HBG285316.
OMA	CRRPTDG.
ProtClustDB	PRK09348.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_CLOD6

Accession

Primary (citable) accession number: Q182B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program