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Protein

Glycine--tRNA ligase beta subunit

Gene

glyS

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-2647-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.14UniRule annotation)
Alternative name(s):
Glycyl-tRNA synthetase beta subunitUniRule annotation
Short name:
GlyRSUniRule annotation
Gene namesi
Name:glySUniRule annotation
Ordered Locus Names:CD630_24320
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688Glycine--tRNA ligase beta subunitPRO_1000101268Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD2432.

Structurei

3D structure databases

ProteinModelPortaliQ182B8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C38. Bacteria.
COG0751. LUCA.
HOGENOMiHOG000264303.
KOiK01879.
OMAiLPIPKRM.
OrthoDBiEOG661H9S.

Family and domain databases

HAMAPiMF_00255. Gly_tRNA_synth_beta.
InterProiIPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
[Graphical view]
PfamiPF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
[Graphical view]
PRINTSiPR01045. TRNASYNTHGB.
TIGRFAMsiTIGR00211. glyS. 1 hit.
PROSITEiPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q182B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNYLLFEIG VEEMPSRFVG STLEQLKNNL SKLFDENRIA FDKINAYGTP
60 70 80 90 100
RRLTLVVEGL SDRQNDLEEE IKGPAKKIAV DAENNLTKPA LGFIKSKGLS
110 120 130 140 150
ESDIYFKQVG KEEYIFATIK QDGKETSEVL KDILPETIKS VVFPKAMRWG
160 170 180 190 200
GKNLKFARPI RWIVALLNDK VLEFDLEGII SSNVTKGHRF LGESTIEVDS
210 220 230 240 250
IEDYFEKLEK NYIVLDQNKR KEMIRKQCIE VANSLGGEVE FDEDLLEEVT
260 270 280 290 300
YLVEYPTAFY GEFDVDYIKL PKEVVITPMK QHQRYFPVLK EGKLLPNFIA
310 320 330 340 350
VRNGDSYRID NVKAGNEKVL EARLADALFF YREDTKRNLE SYIEKLKTVV
360 370 380 390 400
FQVKLGTIYD KTLRLEKLSA DILDSLGLVD EKTDTIRAAK LSKADLVTGM
410 420 430 440 450
VGEFDELQGF MGKEYAKVGG ENDVVSEAIF EHYLPRFAGD ILPKTNAGVA
460 470 480 490 500
LSIADKLDSI AGFFAIGIQP TGSQDPYALR RQALGILSIL MDRKVAVDIK
510 520 530 540 550
VLIEHALDNY SELDFDKEEV VEQIMNFFNE RVKNLFKDLG IRYDVVDAVL
560 570 580 590 600
SSDINDVSDM HMRALELNNW LEKDELVEML TAFNRVSTLA QKAESDKIDE
610 620 630 640 650
SLLKEDAEIK LYNEFKQVKI KVNELLKDKK YGIALDEFAS LRPVVDNLFD
660 670 680
TVMVMDNDEA IKNNRLALLK QVYDTMLEIC DLSKIVYK
Length:688
Mass (Da):78,470
Last modified:July 25, 2006 - v1
Checksum:i2346E232152665A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ69318.1.
RefSeqiWP_011861551.1. NZ_CP010905.1.
YP_001088945.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ69318; CAJ69318; CD630_24320.
GeneIDi4915808.
KEGGicdf:CD630_24320.
pdc:CDIF630_02675.
PATRICi19443329. VBICloDif38397_2551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ69318.1.
RefSeqiWP_011861551.1. NZ_CP010905.1.
YP_001088945.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ182B8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD2432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ69318; CAJ69318; CD630_24320.
GeneIDi4915808.
KEGGicdf:CD630_24320.
pdc:CDIF630_02675.
PATRICi19443329. VBICloDif38397_2551.

Phylogenomic databases

eggNOGiENOG4105C38. Bacteria.
COG0751. LUCA.
HOGENOMiHOG000264303.
KOiK01879.
OMAiLPIPKRM.
OrthoDBiEOG661H9S.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-2647-MONOMER.

Family and domain databases

HAMAPiMF_00255. Gly_tRNA_synth_beta.
InterProiIPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
[Graphical view]
PfamiPF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
[Graphical view]
PRINTSiPR01045. TRNASYNTHGB.
TIGRFAMsiTIGR00211. glyS. 1 hit.
PROSITEiPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 630.

Entry informationi

Entry nameiSYGB_PEPD6
AccessioniPrimary (citable) accession number: Q182B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 25, 2006
Last modified: November 11, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.