Q18253 (DPF2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dipeptidyl peptidase family member 2
EC=3.4.14.-

Gene names

Name:	dpf-2
ORF Names:	C27C12.7

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Removes N-terminal dipeptides sequentially from polypeptides By similarity. Essential for control of distal tip cell migration. Ref.3
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S9B family. DPPIV subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 829	829	Dipeptidyl peptidase family member 2		PRO_0000248535
Regions
	Topological domain	1 – 27	27	Cytoplasmic Potential
	Transmembrane	28 – 48	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	49 – 829	781	Extracellular Potential
Sites
	Active site	691	1	Charge relay system By similarity
	Active site	768	1	Charge relay system By similarity
	Active site	800	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	314	1	N-linked (GlcNAc...) Ref.2 Ref.4
	Glycosylation	359	1	N-linked (GlcNAc...) Potential
	Glycosylation	754	1	N-linked (GlcNAc...) Potential
	Disulfide bond	514 ↔ 533		By similarity
	Disulfide bond	711 ↔ 821		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18253 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B3F76F6DC12E44A5	FASTA	829	94,387
	10 20 30 40 50 60 MENDNYDVEE QGCSVFNGKH GYFARSCCVV FILIICVIFV FSVIFTFMQN PINLNSDNGF 70 80 90 100 110 120 NQTSGNTSSL EATTLKPKFS SLMTTTRRFT FEQLFSGKQF LVDYYDYIWL PDGSFVQMND 130 140 150 160 170 180 DFTIRKQMKK IPLGSSVAEP FFNNGEYVKA LSSNMKYAYG SKKVNELWRH SAEYLYHIVK 190 200 210 220 230 240 INNKTVSTEQ WHVGPEENSL IQAFYWNPNA SSNDFVYVHN YNLYYQKDPE KPDGAIQLTV 250 260 270 280 290 300 GGSTFNRFGL ANWLYEEEIL EASSAVWWSP SGRYVSYLRF DDREVNRIFL PKYTDDDSYV 310 320 330 340 350 360 EYFELPYPKA GVQNNTLVTQ YIWDSENHKI VETAPPNELS AANGDYYVLT NKWITMPRNG 370 380 390 400 410 420 SDLGEERLVT VWANRDQNHV YFSLCNEQDC VMALSFQFSI DNRQLWVSPK DVRGVFPTET 430 440 450 460 470 480 GFLTVLPHKH DDGNIYNHVA HVELDGTGTG KITKWIGENF DVILVLGYSS KIDALTFSAY 490 500 510 520 530 540 GDGVGEFSTY IVREAMYSNK KTTLQKVTDQ FEDCKTLGSQ SADPTGQRIV VQCEKPFDNT 550 560 570 580 590 600 RLYLVDVVDT TKKIMLEGGT KAVIPFDVPN MKFGKLKLPS GIDGHYMMLT PANLLDGAKI 610 620 630 640 650 660 PLLLDIYGGP DSKQVFQKTP TAHAIQIVSQ YDIAYARIDV RGTGGRGWDV KEAVYRKLGD 670 680 690 700 710 720 AEVVDTLDMI RAFINTFGFI DEDRIAVMGW SYGGFLTSKI AIKDQGELVK CAISIAPVTD 730 740 750 760 770 780 FKYYDSAYTE RYLGQPAENL QGYINTNVIP HARNVTNVKY LLAHGERDDN VHYQNSARWS 790 800 810 820 EALQQNGIHF TQLVYANEAH SLSHKLFHLY GEVQRFLMND CFKSNLDLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins." Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T. Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-314, MASS SPECTROMETRY. Strain: Bristol N2.
[3]	"Dipeptidyl peptidase IV-like protease family is essential for control of distal tip cell migration in C. elegans." Yoshina S., Gengyo-Ando K., Mitani S., Iino Y., Inoue H., Takahashi K. (In) Proceedings of the 15th international C. elegans meeting, pp.402-402, Los Angeles (2005) Cited for: FUNCTION.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-314, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z69883 Genomic DNA. Translation: CAA93743.1.
PIR	T19514.
RefSeq	NP_510461.1. NM_078060.6.
3D structure databases
HSSP	HSSP built from PDB template 1Z68 based on UniProtKB Q12884.
ProteinModelPortal	Q18253.
SMR	Q18253. Positions 168-823.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.C27C12.7.
Protein family/group databases
MEROPS	S09.A74.
Proteomic databases
PaxDb	Q18253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C27C12.7; C27C12.7; C27C12.7.
GeneID	181579.
KEGG	cel:CELE_C27C12.7.
UCSC	C27C12.7. c. elegans.
Organism-specific databases
CTD	181579.
WormBase	C27C12.7; CE05324; WBGene00001055; dpf-2.
Phylogenomic databases
eggNOG	COG1506.
GeneTree	ENSGT00530000062751.
HOGENOM	HOG000018328.
InParanoid	Q18253.
OMA	CAISIAP.
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	914522.

Entry information

Entry name

DPF2_CAEEL

Accession

Primary (citable) accession number: Q18253

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project