Probable histone-lysine N-methyltransferase set-2

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable histone-lysine N-methyltransferase set-2
EC=2.1.1.43

Alternative name(s):
SET domain-containing protein 2

Gene names

Name:	set-2
ORF Names:	C26E6.9

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1507 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable histone methyltransferase involved in chromatin modification and/or remodeling in meiotic germ cells. May act redundantly with mes-3 and mes-4 proteins. Required for RNAi. Functions as an antagonist of hpl-1 and hpl-2 activity in growth and somatic gonad development. Ref.2 Ref.3 Ref.4 Ref.5
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus. Note: Localized in mitotic and mid-late-stage meiotic nuclei but is undetectable in early pachytene nuclei. Ref.2
Tissue specificity	Expressed in all cells of embryo. In L1 larva, it is predominantly expressed in Z2 and Z3 primordial germ cells. In adults, it is predominantly expressed in the germline. Ref.2
Developmental stage	Expressed throughout embryogenesis.
Sequence similarities	Contains 1 post-SET domain. Contains 1 RRM (RNA recognition motif) domain. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Chromatin regulator Developmental protein Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	determination of adult lifespan Inferred from mutant phenotype PubMed 20555324. Source: WormBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.2. Source: WormBase
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW histone methyltransferase activity (H3-K4 specific) Inferred from direct assay PubMed 20555324. Source: WormBase nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q18221-1) Also known as: L; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q18221-2) Also known as: S; The sequence of this isoform differs from the canonical sequence as follows: 1-768: Missing. 769-831: MDELSRKVAE...PSNHLIADMM → MYNNSAPYLN...PQRVYRSINS

Isoform c (identifier: Q18221-3) The sequence of this isoform differs from the canonical sequence as follows: 831-831: M → MPSQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1507

1507

Probable histone-lysine N-methyltransferase set-2

PRO_0000097695

Regions

Domain

128 – 199

72

RRM

Domain

1366 – 1489

124

SET

Domain

1491 – 1507

17

Post-SET

Compositional bias

296 – 354

59

Pro-rich

Compositional bias

554 – 664

111

Pro-rich

Compositional bias

870 – 1011

142

Ser-rich

Natural variations

Alternative sequence

1 – 768

768

Missing in isoform b.

VSP_007217

Alternative sequence

769 – 831

63

MDELS…IADMM → MYNNSAPYLNHSSLNTVRKK VVTVRRVLPSLPPPPPPPPS LYPPCSVFKVPYIPQRVYRS INS in isoform b.

VSP_007218

Alternative sequence

831

1

M → MPSQ in isoform c.

VSP_038347

Sequences

Sequence		Length	Mass (Da)
Isoform a (L) [UniParc]. Last modified April 23, 2003. Version 2. Checksum: E7D9689DA720C34A Show »	FASTA	1,507	171,683
10 20 30 40 50 60 MSTHDMNHHP PRKSHSKRDK PSSSNSGPKI ENHKCKWAWQ KVFETGKSFL RRDGFPQDCK 70 80 90 100 110 120 SKEDFERIKR TGVRKTSENM LEDPRKNFES LQQSSVYQTN SFRNPRYLCR AHLRVDSYYC 130 140 150 160 170 180 TIPPKREVSL FNMDDNCTEV LLRDFAKDCG KVEKAYVCIH PETKRHMKMA YVKFATVKEA 190 200 210 220 230 240 HNFYSMYHAQ NLLATKCTPR IDPFLSILNE EYEVATNGQV LPILPDDLAS IDPSVLRDLR 250 260 270 280 290 300 ANFLRDQNEK YELAMRNTYE DEGGMLSGVI MDTSDHYERD YTMDHDVGPS SMKMSPIPPP 310 320 330 340 350 360 PIKEESPPPP PPPPVASVSN LAPVPSVQLP YYNNIQPSSS TMHMPEFRPT EPPPSYSRED 370 380 390 400 410 420 PYRSTSRSSL SRHRNRSRSP SDGMDRSGRS SSRRTHRRPE SRNGSKNANG DVVKYETYKM 430 440 450 460 470 480 EKRKIKYEGG NKKYEQVHIK ERTAVIRGKN QLENVSSESA SGSSSVDTYP DFSDEERKKK 490 500 510 520 530 540 KRPKSPNRSK KDSRAFGWDS TDESDEDTRR RRSGRSQNRS SERKFQTTSS SSTRRELSST 550 560 570 580 590 600 HTNSVPNLKS HETPPPPPPK GHPSVHLQTP YQHVQPQMIP ATYYNLPPQH MAPPPITTSL 610 620 630 640 650 660 PPFCDFSQPP PGFTPTFKPI TNAPLPTPYQ ASNIPQPGLV QIAALSAAPE PFSSIPGPPP 670 680 690 700 710 720 GPAPIQEDVG RAESPEKPSL SERFSGIFGP TQREEPAQVE VEYDYPLKHS ESHDDRHSLE 730 740 750 760 770 780 DMDVEVSSDG ETVSNVEKIE CMEEKKRQDL ERIAIARTPI VKKCKKRMMD ELSRKVAEDI 790 800 810 820 830 840 RQQIMRQCFA ALDEKLHLKA IADEEKRKKE REEKARQEAE KPSNHLIADM MTLYNNQSFA 850 860 870 880 890 900 SSSRGFYRKQ KPIPKSHPKH QEHHHHAKAS VSTPVHSSST SRNSSVAPTP QRTVSTSSSS 910 920 930 940 950 960 SSAATSARVS EDESDSDSTP GEVQRRKTSV LSNDKRRRRA SFSSTSIQSS PERQRDVSSS 970 980 990 1000 1010 1020 SRTSSSSSTS SMKQEETADE KSRKRKLIMS SDESSTTGST ATSVVSSRQS SLEPQQEKTD 1030 1040 1050 1060 1070 1080 GEPPKKKSQT DFISERVSKI EGEERPLPEP VETSGPIIGD SSYLPYKIVH WEKAGIIEMN 1090 1100 1110 1120 1130 1140 LPANSIRAHE YHPFTTEHCY FGIDDPRQPK IQIFDHSPCK SEPGSEPLKI TPAPWGPIDN 1150 1160 1170 1180 1190 1200 VAETGPLIYM DVVTAPKTVQ KKQKPRKQVF EKDPYEYYEP PPTKRPAPPP RFKKTFKPRS 1210 1220 1230 1240 1250 1260 EEEKKKIIGD CEDLPDLEDQ WYLRAALNEM QSEVKSADEL PWKKMLTFKE MLRSEDPLLR 1270 1280 1290 1300 1310 1320 LNPIRSKKGL PDAFYEDEEL DGVIPVAAGC SRARPYEKMT MKQKRSLVRR PDNESHPTAI 1330 1340 1350 1360 1370 1380 FSERDETAIR HQHLASKDMR LLQRRLLTSL GDANNDFFKI NQLKFRKKMI KFARSRIHGW 1390 1400 1410 1420 1430 1440 GLYAMESIAP DEMIVEYIGQ TIRSLVAEER EKAYERRGIG SSYLFRIDLH HVIDATKRGN 1450 1460 1470 1480 1490 1500 FARFINHSCQ PNCYAKVLTI EGEKRIVIYS RTIIKKGEEI TYDYKFPIED DKIDCLCGAK TCRGYLN « Hide
Isoform b (S) [UniParc]. Checksum: 6588F8D83C72BABD Show »	FASTA	739	83,999
10 20 30 40 50 60 MYNNSAPYLN HSSLNTVRKK VVTVRRVLPS LPPPPPPPPS LYPPCSVFKV PYIPQRVYRS 70 80 90 100 110 120 INSTLYNNQS FASSSRGFYR KQKPIPKSHP KHQEHHHHAK ASVSTPVHSS STSRNSSVAP 130 140 150 160 170 180 TPQRTVSTSS SSSSAATSAR VSEDESDSDS TPGEVQRRKT SVLSNDKRRR RASFSSTSIQ 190 200 210 220 230 240 SSPERQRDVS SSSRTSSSSS TSSMKQEETA DEKSRKRKLI MSSDESSTTG STATSVVSSR 250 260 270 280 290 300 QSSLEPQQEK TDGEPPKKKS QTDFISERVS KIEGEERPLP EPVETSGPII GDSSYLPYKI 310 320 330 340 350 360 VHWEKAGIIE MNLPANSIRA HEYHPFTTEH CYFGIDDPRQ PKIQIFDHSP CKSEPGSEPL 370 380 390 400 410 420 KITPAPWGPI DNVAETGPLI YMDVVTAPKT VQKKQKPRKQ VFEKDPYEYY EPPPTKRPAP 430 440 450 460 470 480 PPRFKKTFKP RSEEEKKKII GDCEDLPDLE DQWYLRAALN EMQSEVKSAD ELPWKKMLTF 490 500 510 520 530 540 KEMLRSEDPL LRLNPIRSKK GLPDAFYEDE ELDGVIPVAA GCSRARPYEK MTMKQKRSLV 550 560 570 580 590 600 RRPDNESHPT AIFSERDETA IRHQHLASKD MRLLQRRLLT SLGDANNDFF KINQLKFRKK 610 620 630 640 650 660 MIKFARSRIH GWGLYAMESI APDEMIVEYI GQTIRSLVAE EREKAYERRG IGSSYLFRID 670 680 690 700 710 720 LHHVIDATKR GNFARFINHS CQPNCYAKVL TIEGEKRIVI YSRTIIKKGE EITYDYKFPI 730 EDDKIDCLCG AKTCRGYLN « Hide
Isoform c [UniParc]. Checksum: F301028054840D2E Show »	FASTA	1,510	171,995
10 20 30 40 50 60 MSTHDMNHHP PRKSHSKRDK PSSSNSGPKI ENHKCKWAWQ KVFETGKSFL RRDGFPQDCK 70 80 90 100 110 120 SKEDFERIKR TGVRKTSENM LEDPRKNFES LQQSSVYQTN SFRNPRYLCR AHLRVDSYYC 130 140 150 160 170 180 TIPPKREVSL FNMDDNCTEV LLRDFAKDCG KVEKAYVCIH PETKRHMKMA YVKFATVKEA 190 200 210 220 230 240 HNFYSMYHAQ NLLATKCTPR IDPFLSILNE EYEVATNGQV LPILPDDLAS IDPSVLRDLR 250 260 270 280 290 300 ANFLRDQNEK YELAMRNTYE DEGGMLSGVI MDTSDHYERD YTMDHDVGPS SMKMSPIPPP 310 320 330 340 350 360 PIKEESPPPP PPPPVASVSN LAPVPSVQLP YYNNIQPSSS TMHMPEFRPT EPPPSYSRED 370 380 390 400 410 420 PYRSTSRSSL SRHRNRSRSP SDGMDRSGRS SSRRTHRRPE SRNGSKNANG DVVKYETYKM 430 440 450 460 470 480 EKRKIKYEGG NKKYEQVHIK ERTAVIRGKN QLENVSSESA SGSSSVDTYP DFSDEERKKK 490 500 510 520 530 540 KRPKSPNRSK KDSRAFGWDS TDESDEDTRR RRSGRSQNRS SERKFQTTSS SSTRRELSST 550 560 570 580 590 600 HTNSVPNLKS HETPPPPPPK GHPSVHLQTP YQHVQPQMIP ATYYNLPPQH MAPPPITTSL 610 620 630 640 650 660 PPFCDFSQPP PGFTPTFKPI TNAPLPTPYQ ASNIPQPGLV QIAALSAAPE PFSSIPGPPP 670 680 690 700 710 720 GPAPIQEDVG RAESPEKPSL SERFSGIFGP TQREEPAQVE VEYDYPLKHS ESHDDRHSLE 730 740 750 760 770 780 DMDVEVSSDG ETVSNVEKIE CMEEKKRQDL ERIAIARTPI VKKCKKRMMD ELSRKVAEDI 790 800 810 820 830 840 RQQIMRQCFA ALDEKLHLKA IADEEKRKKE REEKARQEAE KPSNHLIADM MPSQTLYNNQ 850 860 870 880 890 900 SFASSSRGFY RKQKPIPKSH PKHQEHHHHA KASVSTPVHS SSTSRNSSVA PTPQRTVSTS 910 920 930 940 950 960 SSSSSAATSA RVSEDESDSD STPGEVQRRK TSVLSNDKRR RRASFSSTSI QSSPERQRDV 970 980 990 1000 1010 1020 SSSSRTSSSS STSSMKQEET ADEKSRKRKL IMSSDESSTT GSTATSVVSS RQSSLEPQQE 1030 1040 1050 1060 1070 1080 KTDGEPPKKK SQTDFISERV SKIEGEERPL PEPVETSGPI IGDSSYLPYK IVHWEKAGII 1090 1100 1110 1120 1130 1140 EMNLPANSIR AHEYHPFTTE HCYFGIDDPR QPKIQIFDHS PCKSEPGSEP LKITPAPWGP 1150 1160 1170 1180 1190 1200 IDNVAETGPL IYMDVVTAPK TVQKKQKPRK QVFEKDPYEY YEPPPTKRPA PPPRFKKTFK 1210 1220 1230 1240 1250 1260 PRSEEEKKKI IGDCEDLPDL EDQWYLRAAL NEMQSEVKSA DELPWKKMLT FKEMLRSEDP 1270 1280 1290 1300 1310 1320 LLRLNPIRSK KGLPDAFYED EELDGVIPVA AGCSRARPYE KMTMKQKRSL VRRPDNESHP 1330 1340 1350 1360 1370 1380 TAIFSERDET AIRHQHLASK DMRLLQRRLL TSLGDANNDF FKINQLKFRK KMIKFARSRI 1390 1400 1410 1420 1430 1440 HGWGLYAMES IAPDEMIVEY IGQTIRSLVA EEREKAYERR GIGSSYLFRI DLHHVIDATK 1450 1460 1470 1480 1490 1500 RGNFARFINH SCQPNCYAKV LTIEGEKRIV IYSRTIIKKG EEITYDYKFP IEDDKIDCLC 1510 GAKTCRGYLN « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"Depletion of a novel SET-domain protein enhances the sterility of mes-3 and mes-4 mutants of Caenorhabditis elegans." Xu L., Strome S. Genetics 159:1019-1029(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[3]	"A targeted RNAi screen for genes involved in chromosome morphogenesis and nuclear organization in the Caenorhabditis elegans germline." Colaiacovo M.P., Stanfield G.M., Reddy K.C., Reinke V., Kim S.K., Villeneuve A.M. Genetics 162:113-128(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Telomeric position effect variegation in Saccharomyces cerevisiae by Caenorhabditis elegans linker histones suggests a mechanistic connection between germ line and telomeric silencing." Jedrusik M.A., Schulze E. Mol. Cell. Biol. 23:3681-3691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Antagonistic functions of SET-2/SET1 and HPL/HP1 proteins in C. elegans development." Simonet T., Dulermo R., Schott S., Palladino F. Dev. Biol. 312:367-383(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080680 Genomic DNA. Translation: CCD65735.1. FO080680 Genomic DNA. Translation: CCD65734.1. FO080680 Genomic DNA. Translation: CCD65736.1.
PIR	A88445.
RefSeq	NP_498039.1. NM_065638.3. NP_498040.1. NM_065639.4. NP_498041.1. NM_065640.3.
3D structure databases
ProteinModelPortal	Q18221.
SMR	Q18221. Positions 124-222, 1364-1507.
ModBase	Search...
Proteomic databases
PaxDb	Q18221.
PRIDE	Q18221.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C26E6.9a.1; C26E6.9a.1; C26E6.9. C26E6.9a.2; C26E6.9a.2; C26E6.9.
GeneID	175662.
KEGG	cel:CELE_C26E6.9.
UCSC	C26E6.9a. c. elegans.
Organism-specific databases
CTD	175662.
WormBase	C26E6.9a; CE27735; WBGene00004782; set-2. C26E6.9b; CE01158; WBGene00004782; set-2. C26E6.9c; CE27736; WBGene00004782; set-2.
Phylogenomic databases
eggNOG	COG2940.
GeneTree	ENSGT00700000104213.
HOGENOM	HOG000021414.
InParanoid	Q18221.
KO	K11422.
OMA	YCTIPPK.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR015722. Histone-lysine_MeTfrase. IPR012677. Nucleotide-bd_a/b_plait. IPR003616. Post-SET_dom. IPR000504. RRM_dom. IPR001214. SET_dom. [Graphical view]
PANTHER	PTHR22884:SF10. PTHR22884:SF10. 1 hit.
Pfam	PF00076. RRM_1. 1 hit. PF00856. SET. 1 hit. [Graphical view]
SMART	SM00508. PostSET. 1 hit. SM00360. RRM. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50868. POST_SET. 1 hit. PS50102. RRM. False negative. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	889112.

Entry information

Entry name

SET2_CAEEL

Accession

Primary (citable) accession number: Q18221
Secondary accession number(s): Q95QU6, Q95QU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents