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Q18221

- SET2_CAEEL

UniProt

Q18221 - SET2_CAEEL

Protein

Probable histone-lysine N-methyltransferase set-2

Gene

set-2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (23 Apr 2003)
      Previous versions | rss
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    Functioni

    Probable histone methyltransferase involved in chromatin modification and/or remodeling in meiotic germ cells. May act redundantly with mes-3 and mes-4 proteins. Required for RNAi. Functions as an antagonist of hpl-1 and hpl-2 activity in growth and somatic gonad development.4 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

    GO - Molecular functioni

    1. histone methyltransferase activity (H3-K4 specific) Source: WormBase
    2. nucleotide binding Source: InterPro
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. determination of adult lifespan Source: WormBase
    2. histone H3-K4 methylation Source: WormBase
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Developmental protein, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable histone-lysine N-methyltransferase set-2 (EC:2.1.1.43)
    Alternative name(s):
    SET domain-containing protein 2
    Gene namesi
    Name:set-2
    ORF Names:C26E6.9
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiC26E6.9a; CE27735; WBGene00004782; set-2.
    C26E6.9b; CE01158; WBGene00004782; set-2.
    C26E6.9c; CE27736; WBGene00004782; set-2.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Localized in mitotic and mid-late-stage meiotic nuclei but is undetectable in early pachytene nuclei.

    GO - Cellular componenti

    1. nucleus Source: WormBase

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15071507Probable histone-lysine N-methyltransferase set-2PRO_0000097695Add
    BLAST

    Proteomic databases

    PaxDbiQ18221.
    PRIDEiQ18221.

    Expressioni

    Tissue specificityi

    Expressed in all cells of embryo. In L1 larva, it is predominantly expressed in Z2 and Z3 primordial germ cells. In adults, it is predominantly expressed in the germline.1 Publication

    Developmental stagei

    Expressed throughout embryogenesis.

    Interactioni

    Protein-protein interaction databases

    BioGridi40896. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ18221.
    SMRiQ18221. Positions 124-222, 1340-1507.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini128 – 19972RRMAdd
    BLAST
    Domaini1368 – 1485118SETPROSITE-ProRule annotationAdd
    BLAST
    Domaini1491 – 150717Post-SETPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi296 – 35459Pro-richAdd
    BLAST
    Compositional biasi554 – 664111Pro-richAdd
    BLAST
    Compositional biasi870 – 1011142Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
    Contains 1 post-SET domain.PROSITE-ProRule annotation
    Contains 1 SET domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2940.
    GeneTreeiENSGT00740000115089.
    HOGENOMiHOG000021414.
    InParanoidiQ18221.
    KOiK11422.
    OMAiYCTIPPK.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR003616. Post-SET_dom.
    IPR000504. RRM_dom.
    IPR001214. SET_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    PF00856. SET. 1 hit.
    [Graphical view]
    SMARTiSM00508. PostSET. 1 hit.
    SM00360. RRM. 1 hit.
    SM00317. SET. 1 hit.
    [Graphical view]
    PROSITEiPS50868. POST_SET. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q18221-1) [UniParc]FASTAAdd to Basket

    Also known as: L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSTHDMNHHP PRKSHSKRDK PSSSNSGPKI ENHKCKWAWQ KVFETGKSFL     50
    RRDGFPQDCK SKEDFERIKR TGVRKTSENM LEDPRKNFES LQQSSVYQTN 100
    SFRNPRYLCR AHLRVDSYYC TIPPKREVSL FNMDDNCTEV LLRDFAKDCG 150
    KVEKAYVCIH PETKRHMKMA YVKFATVKEA HNFYSMYHAQ NLLATKCTPR 200
    IDPFLSILNE EYEVATNGQV LPILPDDLAS IDPSVLRDLR ANFLRDQNEK 250
    YELAMRNTYE DEGGMLSGVI MDTSDHYERD YTMDHDVGPS SMKMSPIPPP 300
    PIKEESPPPP PPPPVASVSN LAPVPSVQLP YYNNIQPSSS TMHMPEFRPT 350
    EPPPSYSRED PYRSTSRSSL SRHRNRSRSP SDGMDRSGRS SSRRTHRRPE 400
    SRNGSKNANG DVVKYETYKM EKRKIKYEGG NKKYEQVHIK ERTAVIRGKN 450
    QLENVSSESA SGSSSVDTYP DFSDEERKKK KRPKSPNRSK KDSRAFGWDS 500
    TDESDEDTRR RRSGRSQNRS SERKFQTTSS SSTRRELSST HTNSVPNLKS 550
    HETPPPPPPK GHPSVHLQTP YQHVQPQMIP ATYYNLPPQH MAPPPITTSL 600
    PPFCDFSQPP PGFTPTFKPI TNAPLPTPYQ ASNIPQPGLV QIAALSAAPE 650
    PFSSIPGPPP GPAPIQEDVG RAESPEKPSL SERFSGIFGP TQREEPAQVE 700
    VEYDYPLKHS ESHDDRHSLE DMDVEVSSDG ETVSNVEKIE CMEEKKRQDL 750
    ERIAIARTPI VKKCKKRMMD ELSRKVAEDI RQQIMRQCFA ALDEKLHLKA 800
    IADEEKRKKE REEKARQEAE KPSNHLIADM MTLYNNQSFA SSSRGFYRKQ 850
    KPIPKSHPKH QEHHHHAKAS VSTPVHSSST SRNSSVAPTP QRTVSTSSSS 900
    SSAATSARVS EDESDSDSTP GEVQRRKTSV LSNDKRRRRA SFSSTSIQSS 950
    PERQRDVSSS SRTSSSSSTS SMKQEETADE KSRKRKLIMS SDESSTTGST 1000
    ATSVVSSRQS SLEPQQEKTD GEPPKKKSQT DFISERVSKI EGEERPLPEP 1050
    VETSGPIIGD SSYLPYKIVH WEKAGIIEMN LPANSIRAHE YHPFTTEHCY 1100
    FGIDDPRQPK IQIFDHSPCK SEPGSEPLKI TPAPWGPIDN VAETGPLIYM 1150
    DVVTAPKTVQ KKQKPRKQVF EKDPYEYYEP PPTKRPAPPP RFKKTFKPRS 1200
    EEEKKKIIGD CEDLPDLEDQ WYLRAALNEM QSEVKSADEL PWKKMLTFKE 1250
    MLRSEDPLLR LNPIRSKKGL PDAFYEDEEL DGVIPVAAGC SRARPYEKMT 1300
    MKQKRSLVRR PDNESHPTAI FSERDETAIR HQHLASKDMR LLQRRLLTSL 1350
    GDANNDFFKI NQLKFRKKMI KFARSRIHGW GLYAMESIAP DEMIVEYIGQ 1400
    TIRSLVAEER EKAYERRGIG SSYLFRIDLH HVIDATKRGN FARFINHSCQ 1450
    PNCYAKVLTI EGEKRIVIYS RTIIKKGEEI TYDYKFPIED DKIDCLCGAK 1500
    TCRGYLN 1507
    Length:1,507
    Mass (Da):171,683
    Last modified:April 23, 2003 - v2
    Checksum:iE7D9689DA720C34A
    GO
    Isoform b (identifier: Q18221-2) [UniParc]FASTAAdd to Basket

    Also known as: S

    The sequence of this isoform differs from the canonical sequence as follows:
         1-768: Missing.
         769-831: MDELSRKVAE...PSNHLIADMM → MYNNSAPYLN...PQRVYRSINS

    Show »
    Length:739
    Mass (Da):83,999
    Checksum:i6588F8D83C72BABD
    GO
    Isoform c (identifier: Q18221-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         831-831: M → MPSQ

    Note: No experimental confirmation available.

    Show »
    Length:1,510
    Mass (Da):171,995
    Checksum:iF301028054840D2E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 768768Missing in isoform b. CuratedVSP_007217Add
    BLAST
    Alternative sequencei769 – 83163MDELS…IADMM → MYNNSAPYLNHSSLNTVRKK VVTVRRVLPSLPPPPPPPPS LYPPCSVFKVPYIPQRVYRS INS in isoform b. CuratedVSP_007218Add
    BLAST
    Alternative sequencei831 – 8311M → MPSQ in isoform c. CuratedVSP_038347

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080680 Genomic DNA. Translation: CCD65735.1.
    FO080680 Genomic DNA. Translation: CCD65734.1.
    FO080680 Genomic DNA. Translation: CCD65736.1.
    PIRiA88445.
    RefSeqiNP_498039.1. NM_065638.3. [Q18221-3]
    NP_498040.1. NM_065639.4. [Q18221-1]
    NP_498041.1. NM_065640.3. [Q18221-2]
    UniGeneiCel.8145.

    Genome annotation databases

    EnsemblMetazoaiC26E6.9a; C26E6.9a; WBGene00004782. [Q18221-1]
    GeneIDi175662.
    KEGGicel:CELE_C26E6.9.
    UCSCiC26E6.9a. c. elegans. [Q18221-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080680 Genomic DNA. Translation: CCD65735.1 .
    FO080680 Genomic DNA. Translation: CCD65734.1 .
    FO080680 Genomic DNA. Translation: CCD65736.1 .
    PIRi A88445.
    RefSeqi NP_498039.1. NM_065638.3. [Q18221-3 ]
    NP_498040.1. NM_065639.4. [Q18221-1 ]
    NP_498041.1. NM_065640.3. [Q18221-2 ]
    UniGenei Cel.8145.

    3D structure databases

    ProteinModelPortali Q18221.
    SMRi Q18221. Positions 124-222, 1340-1507.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 40896. 2 interactions.

    Proteomic databases

    PaxDbi Q18221.
    PRIDEi Q18221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C26E6.9a ; C26E6.9a ; WBGene00004782 . [Q18221-1 ]
    GeneIDi 175662.
    KEGGi cel:CELE_C26E6.9.
    UCSCi C26E6.9a. c. elegans. [Q18221-1 ]

    Organism-specific databases

    CTDi 175662.
    WormBasei C26E6.9a ; CE27735 ; WBGene00004782 ; set-2.
    C26E6.9b ; CE01158 ; WBGene00004782 ; set-2.
    C26E6.9c ; CE27736 ; WBGene00004782 ; set-2.

    Phylogenomic databases

    eggNOGi COG2940.
    GeneTreei ENSGT00740000115089.
    HOGENOMi HOG000021414.
    InParanoidi Q18221.
    KOi K11422.
    OMAi YCTIPPK.

    Miscellaneous databases

    NextBioi 889112.
    PROi Q18221.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR003616. Post-SET_dom.
    IPR000504. RRM_dom.
    IPR001214. SET_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    PF00856. SET. 1 hit.
    [Graphical view ]
    SMARTi SM00508. PostSET. 1 hit.
    SM00360. RRM. 1 hit.
    SM00317. SET. 1 hit.
    [Graphical view ]
    PROSITEi PS50868. POST_SET. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    2. "Depletion of a novel SET-domain protein enhances the sterility of mes-3 and mes-4 mutants of Caenorhabditis elegans."
      Xu L., Strome S.
      Genetics 159:1019-1029(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
    3. "A targeted RNAi screen for genes involved in chromosome morphogenesis and nuclear organization in the Caenorhabditis elegans germline."
      Colaiacovo M.P., Stanfield G.M., Reddy K.C., Reinke V., Kim S.K., Villeneuve A.M.
      Genetics 162:113-128(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Telomeric position effect variegation in Saccharomyces cerevisiae by Caenorhabditis elegans linker histones suggests a mechanistic connection between germ line and telomeric silencing."
      Jedrusik M.A., Schulze E.
      Mol. Cell. Biol. 23:3681-3691(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Antagonistic functions of SET-2/SET1 and HPL/HP1 proteins in C. elegans development."
      Simonet T., Dulermo R., Schott S., Palladino F.
      Dev. Biol. 312:367-383(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSET2_CAEEL
    AccessioniPrimary (citable) accession number: Q18221
    Secondary accession number(s): Q95QU6, Q95QU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: April 23, 2003
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3