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Protein

Zinc metalloproteinase nas-36

Gene

nas-36

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable metalloprotease involved in molting, a process during larval stages in which a new cuticle is formed and the old cuticle is shed.1 Publication

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi219 – 2191Zinc; catalyticPROSITE-ProRule annotation
Active sitei220 – 2201PROSITE-ProRule annotation
Metal bindingi223 – 2231Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi229 – 2291Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • molting cycle, collagen and cuticulin-based cuticle Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.319.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-36 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 36
Gene namesi
Name:nas-36
ORF Names:C26C6.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiC26C6.3; CE38281; WBGene00003552; nas-36.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Worms exhibit a trail of old cuticle that remains attached to the posterior part of the body.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 617595Zinc metalloproteinase nas-36PRO_0000028940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence analysis
Disulfide bondi321 ↔ 332By similarity
Disulfide bondi325 ↔ 346By similarity
Disulfide bondi348 ↔ 357By similarity
Disulfide bondi368 ↔ 397By similarity
Disulfide bondi425 ↔ 445By similarity
Disulfide bondi519 ↔ 550By similarity
Disulfide bondi523 ↔ 555By similarity
Disulfide bondi535 ↔ 540By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ18206.
PaxDbiQ18206.
PRIDEiQ18206.

Expressioni

Tissue specificityi

Expressed in hypodermal cells. Also detected in the hypodermal seam cells in L4 larvae and young adults. In old adult hermaphrodites, it localizes to the vulva (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.C26C6.3.

Structurei

3D structure databases

ProteinModelPortaliQ18206.
SMRiQ18206. Positions 97-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini317 – 35842EGF-likeAdd
BLAST
Domaini368 – 482115CUBPROSITE-ProRule annotationAdd
BLAST
Domaini507 – 55650TSP type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 CUB domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000020944.
InParanoidiQ18206.
KOiK08076.
OMAiSTCITFE.
OrthoDBiEOG7X3QQK.
PhylomeDBiQ18206.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 1 hit.
SM00209. TSP1. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00022. EGF_1. 1 hit.
PS50092. TSP1. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q18206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLCHSIILF NSLISISICS KADDPALLVA SEFKEHFNVE EKQLETVEEL
60 70 80 90 100
LIKMKKLAHS RSFKGREFGH DAVEDSKKEV AISTQQGTIN KKVSPFLFEG
110 120 130 140 150
DIFLSRRQAV DILKALSKDK TKRLRRSFVS DKTATWKTMP IKYRFHESID
160 170 180 190 200
FYTISQIIAA IRFWEDSTCI TFENVSDSPD GDYIEFFSGQ GCYSMIGRNG
210 220 230 240 250
GRQGISIGES CVKMGVIEHE IGHALGLWHE QSRPDALGYV TIERDFILPS
260 270 280 290 300
YISDFLQRDD EIDTLGIPYD LGSVMHYGST AFSVDQKSKT VVTRDSLYQQ
310 320 330 340 350
TIGQREKLSF YDVATINTAY CKDECKSEKT KCENGGYMRP SKCSECLCPD
360 370 380 390 400
GLGGEKCEKN EDSKNAECGG IIKLTEEWKE IESPNYPDPG YEADQKCSWL
410 420 430 440 450
LKAEKGKRVE IEFIEDFSFL CTSTCVDFVE LKISDDLRNT GFRFCCYDKP
460 470 480 490 500
EISFVSQTDT AIIIFRSQLS TDIGFKIQAK STDAEPRTTI APTIITTTLA
510 520 530 540 550
PITVDAPNVW ADWGEWSMCS RTCGGCGIRS RVRSCRSKKC EGRRQEFGTC
560 570 580 590 600
NLKACPVDKH CAKLLSNNRL CNGKVCTKND IAISSCDAPQ CCPPFINVDG
610
VCQSDQENQH DELWLSI
Length:617
Mass (Da):69,462
Last modified:December 20, 2005 - v2
Checksum:i4970A25BF228E719
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72503 Genomic DNA. Translation: CAA96596.2.
PIRiT19477.
RefSeqiNP_492109.2. NM_059708.3.
UniGeneiCel.5194.

Genome annotation databases

EnsemblMetazoaiC26C6.3; C26C6.3; WBGene00003552.
GeneIDi172506.
KEGGicel:CELE_C26C6.3.
UCSCiC26C6.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72503 Genomic DNA. Translation: CAA96596.2.
PIRiT19477.
RefSeqiNP_492109.2. NM_059708.3.
UniGeneiCel.5194.

3D structure databases

ProteinModelPortaliQ18206.
SMRiQ18206. Positions 97-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C26C6.3.

Protein family/group databases

MEROPSiM12.319.

Proteomic databases

EPDiQ18206.
PaxDbiQ18206.
PRIDEiQ18206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC26C6.3; C26C6.3; WBGene00003552.
GeneIDi172506.
KEGGicel:CELE_C26C6.3.
UCSCiC26C6.3. c. elegans.

Organism-specific databases

CTDi172506.
WormBaseiC26C6.3; CE38281; WBGene00003552; nas-36.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000020944.
InParanoidiQ18206.
KOiK08076.
OMAiSTCITFE.
OrthoDBiEOG7X3QQK.
PhylomeDBiQ18206.

Miscellaneous databases

NextBioi875811.
PROiQ18206.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 1 hit.
SM00209. TSP1. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00022. EGF_1. 1 hit.
PS50092. TSP1. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "The astacin protein family in Caenorhabditis elegans."
    Moehrlen F., Hutter H., Zwilling R.
    Eur. J. Biochem. 270:4909-4920(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, NOMENCLATURE.
  3. "Metalloproteases with EGF, CUB, and thrombospondin-1 domains function in molting of Caenorhabditis elegans."
    Suzuki M., Sagoh N., Iwasaki H., Inoue H., Takahashi K.
    Biol. Chem. 385:565-568(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiNAS36_CAEEL
AccessioniPrimary (citable) accession number: Q18206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 20, 2005
Last modified: May 11, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.