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Protein

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Gene

gpmI

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = 3-phospho-D-glycerate.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Manganese 2UniRule annotation1
Active sitei62Phosphoserine intermediateUniRule annotation1
Metal bindingi62Manganese 2UniRule annotation1
Binding sitei121SubstrateUniRule annotation1
Binding sitei183SubstrateUniRule annotation1
Binding sitei189SubstrateUniRule annotation1
Binding sitei331SubstrateUniRule annotation1
Metal bindingi398Manganese 1UniRule annotation1
Metal bindingi402Manganese 1UniRule annotation1
Metal bindingi439Manganese 2UniRule annotation1
Metal bindingi440Manganese 2UniRule annotation1
Metal bindingi458Manganese 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-3337-MONOMER.
UniPathwayiUPA00109; UER00186.

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-bisphosphoglycerate-independent phosphoglycerate mutaseUniRule annotation (EC:5.4.2.12UniRule annotation)
Short name:
BPG-independent PGAMUniRule annotation
Short name:
PhosphoglyceromutaseUniRule annotation
Short name:
iPGMUniRule annotation
Gene namesi
Name:gpmIUniRule annotation
Ordered Locus Names:CD630_31710
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000639551 – 5102,3-bisphosphoglycerate-independent phosphoglycerate mutaseAdd BLAST510

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD3171.

Structurei

3D structure databases

ProteinModelPortaliQ181T7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 152Substrate bindingUniRule annotation2
Regioni258 – 261Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the BPG-independent phosphoglycerate mutase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CJI. Bacteria.
COG0696. LUCA.
HOGENOMiHOG000223664.
KOiK15633.
OMAiLHIATMT.

Family and domain databases

Gene3Di3.40.1450.10. 1 hit.
3.40.720.10. 2 hits.
HAMAPiMF_01038. GpmI. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR011258. BPG-indep_PGM_N.
IPR006124. Metalloenzyme.
IPR005995. Pgm_bpd_ind.
[Graphical view]
PfamiPF06415. iPGM_N. 1 hit.
PF01676. Metalloenzyme. 1 hit.
[Graphical view]
PIRSFiPIRSF001492. IPGAM. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.
SSF64158. SSF64158. 1 hit.
TIGRFAMsiTIGR01307. pgm_bpd_ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Q181T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKKPVALII MDGFGYNKDV KGNAIAESKT PNLDRIKKEY PNTLINASGL
60 70 80 90 100
DVGLPDGQMG NSEVGHTNIG AGRIVYQDLT RITKSIKDGD FFTNKVLCEA
110 120 130 140 150
MDNAKENSLH VMGLLSDGGV HSHIDHLKAI IKMAKDKGVQ KVYVHAFTDG
160 170 180 190 200
RDTDPQSALE YAKEVQASMD EIGVGEFATV SGRYYAMDRD KRWERVELAY
210 220 230 240 250
NAMVRGIGEK ANSIEEAIQN SYDDGKNDEF IMPTVIMKDD KPVGSIKEND
260 270 280 290 300
SIIFFNFRPD RARQITRALV CEEFDGFKRE DIKNFFVCLT EYDITIENVH
310 320 330 340 350
IAFGPQSLAN TLGEYLAKNG KTQLRAAETE KYAHVTFFFN GGVEEPNKGE
360 370 380 390 400
ERLLIPSPKV ATYDLKPEMS AYELTDKALD KLGEDKFDFI VLNFANPDMV
410 420 430 440 450
GHTGSIEAAI KAVETVDTCV GKLIDKIVEL GGSAIITADH GNAEYMLDPE
460 470 480 490 500
TGKTVTAHSI NPVPFIVVGQ EYESAKLLDG GRLSDIAPTI LDMMKLEKPE
510
EMTGHSLISK
Length:510
Mass (Da):56,374
Last modified:July 25, 2006 - v1
Checksum:i5AD88452945333F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ70068.1.
RefSeqiWP_011861866.1. NZ_CP010905.1.
YP_001089688.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ70068; CAJ70068; CD630_31710.
GeneIDi4913219.
KEGGicdf:CD630_31710.
PATRICi19444877. VBICloDif38397_3322.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ70068.1.
RefSeqiWP_011861866.1. NZ_CP010905.1.
YP_001089688.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ181T7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD3171.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ70068; CAJ70068; CD630_31710.
GeneIDi4913219.
KEGGicdf:CD630_31710.
PATRICi19444877. VBICloDif38397_3322.

Phylogenomic databases

eggNOGiENOG4105CJI. Bacteria.
COG0696. LUCA.
HOGENOMiHOG000223664.
KOiK15633.
OMAiLHIATMT.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00186.
BioCyciPDIF272563:G12WB-3337-MONOMER.

Family and domain databases

Gene3Di3.40.1450.10. 1 hit.
3.40.720.10. 2 hits.
HAMAPiMF_01038. GpmI. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR011258. BPG-indep_PGM_N.
IPR006124. Metalloenzyme.
IPR005995. Pgm_bpd_ind.
[Graphical view]
PfamiPF06415. iPGM_N. 1 hit.
PF01676. Metalloenzyme. 1 hit.
[Graphical view]
PIRSFiPIRSF001492. IPGAM. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.
SSF64158. SSF64158. 1 hit.
TIGRFAMsiTIGR01307. pgm_bpd_ind. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGPMI_PEPD6
AccessioniPrimary (citable) accession number: Q181T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.