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Q181G8 (ISPE_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:CD630_35660
OrganismClostridium difficile (strain 630) [Complete proteome] [HAMAP]
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2962964-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
PRO_1000092080

Regions

Nucleotide binding95 – 10511ATP Potential

Sites

Active site111 By similarity
Active site1371 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q181G8 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: F9D841C4C1D11A7A

FASTA29632,943
        10         20         30         40         50         60 
MDFIRLKSRA KINLSIDVLG KRQDGYHFVE MIMQTIDLYD IVKIKELDED EIKVKSTSLD 

        70         80         90        100        110        120 
IPLDEDNIVY KAAKILKNKF YIKKGVEIFI EKNIPVAAGM AGGSSNAAAV LVGLNHLWEL 

       130        140        150        160        170        180 
RLSEDELKEI GLNLGADVPF CISGRPALAQ GIGEKLTNIK GLPCDTNILI CKPDLFVSTK 

       190        200        210        220        230        240 
EVYQGLDLNN IKKRPNNKYL IECLKSEDIK AVSESMVNIL ENVTIGKHKE ISDIKQVMMK 

       250        260        270        280        290 
NNALGSMMSG SGPTVFGLFK NKEDALIGKK ELLKKYKQVY VVNSSQKGVE ICGEFN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM180355 Genomic DNA. Translation: CAJ70472.1.
RefSeqYP_001090089.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ181G8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ181G8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4914205.
GenomeReviewsGene locus CD3566 in contig AM180355_GR.
KEGGcdf:CD3566.
NMPDRfig|1496.1.peg.3800.
PATRIC19445717. VBICloDif38397_3736.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1947.
HOGENOMHBG734593.
OMAVSTAEVY.
ProtClustDBPRK00128.

Family and domain databases

HAMAPMF_00061. IspE.
[Tree]
InterProIPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR000870. Homoserine_kinase.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
Gene3DG3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KOK00919.
PANTHERPTHR20861:SF2. IspE. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
PRINTSPR00958. HOMSERKINASE.
SUPFAMSSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMsTIGR00154. IspE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_CLOD6
AccessionPrimary (citable) accession number: Q181G8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 25, 2006
Last modified: January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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