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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 326ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-3825-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:CD630_35600
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462tRNA(Ile)-lysidine synthasePRO_1000065607Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272563.CD3560.

Structurei

3D structure databases

ProteinModelPortaliQ181G3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236506.
KOiK04075.
OMAiNDISYPL.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q181G3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFDKVLSTI NKHNLIQKGD KIVLGLSGGP DSVCLLHVLN RLKKDFNIEI
60 70 80 90 100
YAAHLNHQIR GIEAQKDALY VSKLCEDMGI IFFVKSINVP KYCENEGLSL
110 120 130 140 150
EEGARKLRYE MFYEIKDKIK ANKIAIGHNL NDQAETVMMR IMRGTGLKGL
160 170 180 190 200
KGIDYIRDNC IIRPILDVER NEIEEYCEAY NLNPRIDKTN LENIYTRNKI
210 220 230 240 250
RLDLLPYMKD NFNSNVIESI VRMSNSLKSD NDYIEKEAEA KFREVSNIKE
260 270 280 290 300
KGFVEINLDD FVCLHDAIKV RVLRNSIKHI LGDTNFVDQR HIEDIMSLED
310 320 330 340 350
NSKVNKMLTL PRNIFVYRKK DSIILTNEEI VNEEIEFYYN VPSNGFIKIK
360 370 380 390 400
ELKQIIETQV MSIDRYKSMK LDNSSKGFDF NKVKGGIVIR SRRQGDKIKL
410 420 430 440 450
AMGSKKVKDL FIDLKIPREE RCKIPIITDS EGIICVGDYK ISENYKIDEN
460
TKEVLKINFN KL
Length:462
Mass (Da):53,595
Last modified:July 25, 2006 - v1
Checksum:i2B30BCD6BE00AA36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ70466.1.
RefSeqiWP_003434952.1. NZ_CP010905.1.
YP_001090083.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ70466; CAJ70466; CD630_35600.
GeneIDi4914199.
KEGGicdf:CD630_35600.
pdc:CDIF630_03880.
PATRICi19445705. VBICloDif38397_3730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ70466.1.
RefSeqiWP_003434952.1. NZ_CP010905.1.
YP_001090083.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ181G3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD3560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ70466; CAJ70466; CD630_35600.
GeneIDi4914199.
KEGGicdf:CD630_35600.
pdc:CDIF630_03880.
PATRICi19445705. VBICloDif38397_3730.

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236506.
KOiK04075.
OMAiNDISYPL.
OrthoDBiEOG693GNP.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-3825-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 630.

Entry informationi

Entry nameiTILS_PEPD6
AccessioniPrimary (citable) accession number: Q181G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 25, 2006
Last modified: November 11, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.