Reviewed, UniProtKB/Swiss-Prot Q18164 (DPYD_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable dihydropyrimidine dehydrogenase [NADP+]
      Short name=DHPDHase
      Short name=DPD
    EC=1.3.1.2
Alternative name(s):
    Dihydrouracil dehydrogenase
    Dihydrothymine dehydrogenase

Gene names

ORF Names:

C25F6.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1059 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine By similarity.
Catalytic activity	5,6-dihydrouracil + NADP(+) = uracil + NADPH.
Cofactor	Binds 2 4Fe-4S clusters By similarity. FAD By similarity. FMN By similarity.
Pathway	Amino-acid biosynthesis; beta-alanine biosynthesis.
Sequence similarities	Belongs to the dihydropyrimidine dehydrogenase family. Contains 3 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Domain	Repeat
Ligand	4Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW thymidine catabolic process Inferred from sequence or structural similarity. Source: UniProtKB uracil catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro dihydropyrimidine dehydrogenase (NADP+) activity Inferred from sequence or structural similarity. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1059	1059	Probable dihydropyrimidine dehydrogenase [NADP+]		PRO_0000079997
Regions
	Domain	84 – 118	35	4Fe-4S ferredoxin-type 1
	Domain	955 – 987	33	4Fe-4S ferredoxin-type 2
	Domain	989 – 1019	31	4Fe-4S ferredoxin-type 3
Sites
	Metal binding	964	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	967	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	970	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	974	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	998	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	1001	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	1004	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	1008	1	Iron-sulfur 2 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q18164-1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: E5DDEEE1ED145262	FASTA	1,059	115,301
	10 20 30 40 50 60 MTPKPNTTSP TNNLPLLSKD SPDIESLLIL NPKVQDKANA VPSAVTKKNK HNWKRNEEKG 70 80 90 100 110 120 CGSTCGESKL KNDFRDIKHT TLSERGALKE AMRCLKCADA PCQKSCPTQL DVKSFITSIS 130 140 150 160 170 180 NKNYYGAARQ ILSDNPLGLT CGMICPTSDL CVGSCNLQAS EEGAINIGGL QQYACDVFKQ 190 200 210 220 230 240 MNVRQIVSKE VRENRNASHK EQVALIGCGP ASISCASFLA RLGYTDITIY EKRAYIGGLS 250 260 270 280 290 300 SAEIPQFRLP YDVVDFEIQL ARDIGVQIET NRPLGKDGLT LAKLKEQGAA AVFIGIGNPE 310 320 330 340 350 360 PKIDPLFEGL TIENGFYTSK NYLPAVAAAS KPGMCGCKRT PLPTMRGRVV VLGAGDTAMD 370 380 390 400 410 420 CATSALRCGA SRVTIAFRKG FTGIRAVPEE MEAAKEEKCE FLPFSAPRKI NVKDGRIVSI 430 440 450 460 470 480 EFNKTEQDDN GKWYEDEEQI VILKCDYVIS AFGSTLKEDA VLSALQPCQL NKWGGIEVDS 490 500 510 520 530 540 TTQQTSEKWV FAGGDVAGVA ETTVESVNDG KIAAWNMHRY IQSLHGNQVS ETPELPQFFT 550 560 570 580 590 600 PIDEVDISVD MCGVKFENPF GLASAPPTTS GPMCRRAFEQ GWGFILTKTY GLDKDLVTNV 610 620 630 640 650 660 SPRIVRGSTS GPLYGPNQGS FMNIELISEK SCEYWLQCIR ELKRDHPTKI VIASIMCVYN 670 680 690 700 710 720 KADWIELATK SEEAGADILE LNLSCPHGMG EKGMGLACGQ SPEIVKEICR WVRACVKIPF 730 740 750 760 770 780 FPKMTPNITD VREIARAARD GGASGVTATN TVSSLMHMKA DGNAWPAIGS TKRTTYGGMS 790 800 810 820 830 840 GSAIRPIAMK AVSSIANELD GFPIMATGGI ESAETGLGFL MAGASVLQVC SAVQNQDFTV 850 860 870 880 890 900 VDDYCTGLKA LLYLSGAESL KNWDGQSPPI EKHQKGKPIL LQGQKKMPFF GKYRDEREKL 910 920 930 940 950 960 EAIKLSESNL LDTENYHFAS RPDTQVSRVP TVEDVIGKAL PRIGPYVTLD NQEQKVAIID 970 980 990 1000 1010 1020 DDMCINCGKC YMTCNDSGYQ AITFDPVTHQ PHVTEDDCTG CTLCYSVCPI PECIEMVPRT 1030 1040 1050 GPWKAPKRGV KPSVEPGTPK VVKVDQRGRV ILDTTGGMQ

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U39742 Genomic DNA. Translation: AAK39195.2.
PIR	T15616.
RefSeq	NP_508927.2.
UniGene	Cel.5583
3D structure databases
HSSP	HSSP built from PDB template 1GTE based on UniProtKB Q28943.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:25332N.
IntAct	Q18164.
Genome annotation databases
Ensembl	C25F6.3. Caenorhabditis elegans. [Contig view]
GeneID	180818.
KEGG	cel:C25F6.3.
NMPDR	fig|6239.3.peg.23326.
Organism-specific databases
WormBase	WBGene00016103. C25F6.3.
WormPep	C25F6.3. CE38489. [WorfDB]
Gene expression databases
ArrayExpress	Q18164.
Family and domain databases
InterPro	IPR001450. 4Fe4S_Fe_S_bd. IPR000759. Adrndx_reductase. IPR013785. Aldolase_TIM. IPR005720. DHO_DHase_1_core. IPR001295. Dihydroorotate_DHase_core. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR012285. Fum_reductase_C. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. PF00037. Fer4. 2 hits. PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN. PR00419. ADXRDTASE. PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	911096.

Entry information

Entry name

DPYD_CAEEL

Accession

Primary (citable) accession number: Q18164

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 16, 2006
Last modified:	November 25, 2008
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project