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Protein

TWiK family of potassium channels protein 18

Gene

twk-18

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Outwardly rectifying potassium channel protein; activity is sharply augmented by increase in temperature.1 Publication

GO - Molecular functioni

GO - Biological processi

  • locomotion Source: WormBase
  • muscle contraction Source: WormBase
  • potassium ion transport Source: WormBase
  • stabilization of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-CEL-1299344. TWIK-related spinal cord K+ channel (TRESK).

Protein family/group databases

TCDBi1.A.1.9.5. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
TWiK family of potassium channels protein 18
Gene namesi
Name:twk-18
ORF Names:C24A3.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC24A3.6; CE24811; WBGene00006672; twk-18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicSequence analysisAdd
BLAST
Transmembranei22 – 4221HelicalSequence analysisAdd
BLAST
Intramembranei116 – 13621Pore-forming; Name=Pore-forming 1Sequence analysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence analysisAdd
BLAST
Topological domaini165 – 22460CytoplasmicSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST
Intramembranei253 – 27321Pore-forming; Name=Pore-forming 2Sequence analysisAdd
BLAST
Transmembranei281 – 30121HelicalSequence analysisAdd
BLAST
Topological domaini302 – 461160CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Worms display uncoordinated movement and paralysis.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi140 – 1401G → E in sa589; defects in locomotion. 1 Publication
Mutagenesisi165 – 1651G → D in e1913; homozygotes are embryonic lethal, heterozygotes exhibit defects in locomotion. 1 Publication
Mutagenesisi280 – 2801M → I in cn110; temperature sensitive defects in locomotion. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461TWiK family of potassium channels protein 18PRO_0000101773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ18120.
PaxDbiQ18120.

PTM databases

iPTMnetiQ18120.

Expressioni

Tissue specificityi

Expressed in body wall muscle.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.C24A3.6.2.

Structurei

3D structure databases

ProteinModelPortaliQ18120.
SMRiQ18120. Positions 25-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1418. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00700000104522.
HOGENOMiHOG000015943.
InParanoidiQ18120.
KOiK05323.
OMAiNIDQEYA.
OrthoDBiEOG744T8R.
PhylomeDBiQ18120.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
InterProiIPR003280. 2pore_dom_K_chnl.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR013099. K_chnl_dom.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.

Sequencei

Sequence statusi: Complete.

Q18120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIVAQGVST ILTTFQKTFK GLLPLIILVA YTLLGAWIFW MIEGENEREM
60 70 80 90 100
LIEQQKERDE LIRRTVYKIN QLQIKRQRRL MTAEEEYNRT AKVLTTFQET
110 120 130 140 150
LGIVPADMDK DIHWTFLGSI FYCMTVYTTI GYGNIVPGTG WGRFATILYA
160 170 180 190 200
FIGIPLTVLS LYCLGSLFAK GCKMLWRFFL KSTRVVSKDL SNKISEAADN
210 220 230 240 250
IEEGTTAITP SAEKTENNDD DLLSFPISGL LLITVIWVIF CAVLFTFLEE
260 270 280 290 300
WDFGTSLYFT LISFTTIGFG DILPSDYDFM PIVGVLLLIG LSLVSTVMTL
310 320 330 340 350
IQQQIEALAS GMKDNIDQEY ARALNEARED GEVDEHVDPE EDPENNKKSF
360 370 380 390 400
DAVISRMNWS KRGLYYLLPD SQKKELAKQS EKKMGRKSIK IQTDNDLLET
410 420 430 440 450
LIREEILKAE LNNEMHKYTA PRSSHQPKLV YSDVREKEVP IEVVRVEHFN
460
HGNEDYLEHD I
Length:461
Mass (Da):52,746
Last modified:October 1, 2001 - v2
Checksum:iD436B3654AB9C5DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083650 mRNA. Translation: AAC32861.1.
FO080639 Genomic DNA. Translation: CCD65384.1.
PIRiT15585.
T43394.
RefSeqiNP_509516.1. NM_077115.5.
UniGeneiCel.7698.

Genome annotation databases

EnsemblMetazoaiC24A3.6.1; C24A3.6.1; WBGene00006672.
C24A3.6.2; C24A3.6.2; WBGene00006672.
GeneIDi181139.
KEGGicel:CELE_C24A3.6.
UCSCiC24A3.6.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083650 mRNA. Translation: AAC32861.1.
FO080639 Genomic DNA. Translation: CCD65384.1.
PIRiT15585.
T43394.
RefSeqiNP_509516.1. NM_077115.5.
UniGeneiCel.7698.

3D structure databases

ProteinModelPortaliQ18120.
SMRiQ18120. Positions 25-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C24A3.6.2.

Protein family/group databases

TCDBi1.A.1.9.5. the voltage-gated ion channel (vic) superfamily.

PTM databases

iPTMnetiQ18120.

Proteomic databases

EPDiQ18120.
PaxDbiQ18120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC24A3.6.1; C24A3.6.1; WBGene00006672.
C24A3.6.2; C24A3.6.2; WBGene00006672.
GeneIDi181139.
KEGGicel:CELE_C24A3.6.
UCSCiC24A3.6.2. c. elegans.

Organism-specific databases

CTDi181139.
WormBaseiC24A3.6; CE24811; WBGene00006672; twk-18.

Phylogenomic databases

eggNOGiKOG1418. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00700000104522.
HOGENOMiHOG000015943.
InParanoidiQ18120.
KOiK05323.
OMAiNIDQEYA.
OrthoDBiEOG744T8R.
PhylomeDBiQ18120.

Enzyme and pathway databases

ReactomeiR-CEL-1299344. TWIK-related spinal cord K+ channel (TRESK).

Miscellaneous databases

PROiQ18120.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
InterProiIPR003280. 2pore_dom_K_chnl.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR013099. K_chnl_dom.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutants of a temperature-sensitive two-P domain potassium channel."
    Kunkel M.T., Johnstone D.B., Thomas J.H., Salkoff L.
    J. Neurosci. 20:7517-7524(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-140; GLY-165 AND MET-280, DISRUPTION PHENOTYPE.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-88, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiTWK18_CAEEL
AccessioniPrimary (citable) accession number: Q18120
Secondary accession number(s): Q9TZP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 1, 2001
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.