Q180T6 (COBT_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Short name=NN:DBI PRT
EC=2.4.2.21

Alternative name(s):
N(1)-alpha-phosphoribosyltransferase

Gene names

Name:	cobT
Ordered Locus Names:	CD630_34390

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB). HAMAP MF_00230
Catalytic activity	Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate. HAMAP MF_00230
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2. HAMAP MF_00230
Sequence similarities	Belongs to the CobT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase HAMAP MF_00230		PRO_1000021584
Sites
	Active site	317	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q180T6 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: DA30A3A03A573C5E	FASTA	348	37,354
	10 20 30 40 50 60 MSLLESISKN IYSLDNSSIE KTKQRLDRLI HPTGSLGKIE DICMQLAGIF GNENFDTSKK 70 80 90 100 110 120 VIIAFAGDHG VYEEGVAPDP QNITKLQFPN FSKGLCGVGV ISKFVGADVV AVDVGINCDE 130 140 150 160 170 180 KLDGVLDYKI RKGTSNMAKG PAMSKQEAIR CLEIGIEIAE QCIEKDYKVI GIGEMGIANT 190 200 210 220 230 240 TPSTAIISVI SGCDPLEVTG IGAGLKKERL KHKAEVIRKA IEINNPNPTD GVDILSKVGG 250 260 270 280 290 300 FEIGSMAGVI LGCSANRIPV VIDGFISYAA ALIAYKINPK TREYMIASHL SAESGTKRAL 310 320 330 340 DILKLDPLLN MDMRLGEGSG AALAFDIIEA SNYTYKNMAT FDEIDMGR

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 630.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180355 Genomic DNA. Translation: CAJ70342.1.
RefSeq	YP_001089959.1. NC_009089.1.
3D structure databases
ProteinModelPortal	Q180T6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q180T6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4914770.
GenomeReviews	Gene locus CD3439 in contig AM180355_GR.
KEGG	cdf:CD3439.
NMPDR	fig|1496.1.peg.1090.
PATRIC	19445443. VBICloDif38397_3602.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2038.
HOGENOM	HBG634399.
OMA	KGFAMAR.
ProtClustDB	CLSK2535604.
Family and domain databases
HAMAP	MF_00230. CobT. [Tree]
InterPro	IPR003200. Nict_dMeBzImd_PRibTrfase-like. IPR023195. Nict_dMeBzImd_PRibTrfase_N. IPR017846. Nict_dMeBzImd_PRibTrfase_pro. [Graphical view]
Gene3D	G3DSA:1.10.1610.10. Nict_dMeBzImd_PRibTrfase_N. 1 hit.
KO	K00768.
Pfam	PF02277. DBI_PRT. 1 hit. [Graphical view]
SUPFAM	SSF52733. NN:DBI_PRT. 1 hit.
TIGRFAMs	TIGR03160. CobT_DBIPRT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBT_CLOD6

Accession

Primary (citable) accession number: Q180T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program