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Protein

Adenosylcobinamide-GDP ribazoletransferase

Gene

cobS

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.UniRule annotation

Catalytic activityi

Adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin.UniRule annotation
Adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate = GMP + adenosylcobalamin 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-3695-MONOMER.
UniPathwayiUPA00148; UER00238.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylcobinamide-GDP ribazoletransferaseUniRule annotation (EC:2.7.8.26UniRule annotation)
Alternative name(s):
Cobalamin synthaseUniRule annotation
Cobalamin-5'-phosphate synthaseUniRule annotation
Gene namesi
Name:cobSUniRule annotation
Ordered Locus Names:CD630_34370
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei30 – 5021HelicalUniRule annotationAdd
BLAST
Transmembranei52 – 7221HelicalUniRule annotationAdd
BLAST
Transmembranei109 – 12921HelicalUniRule annotationAdd
BLAST
Transmembranei132 – 15221HelicalUniRule annotationAdd
BLAST
Transmembranei175 – 19521HelicalUniRule annotationAdd
BLAST
Transmembranei198 – 21821HelicalUniRule annotationAdd
BLAST
Transmembranei237 – 25721HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Adenosylcobinamide-GDP ribazoletransferasePRO_1000072777Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272563.CD3437.

Structurei

3D structure databases

ProteinModelPortaliQ180T5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CobS family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107XPA. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
KOiK02233.
OMAiLDGFMDV.

Family and domain databases

HAMAPiMF_00719. CobS. 1 hit.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q180T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRFILILQF LTRIPIKLNV GFDDEFYKSI VYFPLVGFVI GILSYLIGWI
60 70 80 90 100
SMLLFEPFIA SIIITLAGVL ITGGLHIDGL GDTFDAIYSY RDKEKMLEIM
110 120 130 140 150
KDSRLGTNSL LAIMFVLLLK VGFVYDIISN NSLWVIIFMP MIARLGVMLL
160 170 180 190 200
TYKTVTPREK GMGNLFIGKL TTSMLITAII YTLLIVALIT KFIFLLPNIV
210 220 230 240 250
LIKVLGSIIV VFVFIILFKK HIYKKIDGVT GDILGCGIEL SELVYLIYIY

LLIFMFF
Length:257
Mass (Da):29,186
Last modified:July 25, 2006 - v1
Checksum:iBA3F034A3B021AB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ70340.1.
RefSeqiWP_003437766.1. NZ_CP010905.1.
YP_001089957.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ70340; CAJ70340; CD630_34370.
GeneIDi4914768.
KEGGicdf:CD630_34370.
pdc:CDIF630_03746.
PATRICi19445439. VBICloDif38397_3600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ70340.1.
RefSeqiWP_003437766.1. NZ_CP010905.1.
YP_001089957.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ180T5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD3437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ70340; CAJ70340; CD630_34370.
GeneIDi4914768.
KEGGicdf:CD630_34370.
pdc:CDIF630_03746.
PATRICi19445439. VBICloDif38397_3600.

Phylogenomic databases

eggNOGiENOG4107XPA. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
KOiK02233.
OMAiLDGFMDV.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00238.
BioCyciCDIF272563:GJFE-3695-MONOMER.

Family and domain databases

HAMAPiMF_00719. CobS. 1 hit.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOBS_PEPD6
AccessioniPrimary (citable) accession number: Q180T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.