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Q180A1 (Q180A1_CLOD6) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Valine--tRNA ligase HAMAP-Rule MF_02004
EC=6.1.1.9 HAMAP-Rule MF_02004
Alternative name(s):
Valyl-tRNA synthetase HAMAP-Rule MF_02004
Gene names
Name:valS HAMAP-Rule MF_02004 EMBL CAJ70153.1
Ordered Locus Names:CD630_32560 EMBL CAJ70153.1
OrganismClostridium difficile (strain 630) [Complete proteome] [HAMAP] EMBL CAJ70153.1
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Peptostreptococcaceae ›

Protein attributes

Sequence length888 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004 SAAS SAAS019499

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02004 SAAS SAAS019499.

Domain

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. HAMAP-Rule MF_02004

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Coiled coil814 – 88875 By similarity HAMAP-Rule MF_02004
Motif47 – 5711"HIGH" region By similarity HAMAP-Rule MF_02004
Motif527 – 5315"KMSKS" region By similarity HAMAP-Rule MF_02004

Sites

Binding site5301ATP By similarity HAMAP-Rule MF_02004

Sequences

Sequence LengthMass (Da)Tools
Q180A1 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 1FD76D3C708B5D9D

FASTA888103,001
        10         20         30         40         50         60 
MENTNLSKTY NPKDFEARLY KKWMDEGYFK SKPNPDKKPF TIMMPPPNIT GQLHMGHALD 

        70         80         90        100        110        120 
HTLQDILIRW KRMDGYEAFW LPGTDHASIA TEVKVVERIK KQEGKTKYEI GREEFLKRAW 

       130        140        150        160        170        180 
EWKDEFGGKI SNQLKQLGDS CDWDKERFTM DEGCNEAVIE FFVSLYEKGH IYRGNRIINW 

       190        200        210        220        230        240 
CPDCKTTLSD AEVEHEEHDG NFYHIKYPLK DSEDFLEIAT TRPETMIGDT GIAVNPEDDR 

       250        260        270        280        290        300 
YKHLIGKTAI LPLVGRELPI VADSYVDLEF GTGAVKMTPA HDPNDFEVGL RHNLEQLNTM 

       310        320        330        340        350        360 
NEDGTMNEVC GKYEGMDRFE CRKAIVADLK EQGYLIKIKE HNHNVGTCYR CHTVVEPRLS 

       370        380        390        400        410        420 
EQWFVKMEEL AKPAIDILKK GELEFVPDKF DKTYLQWLEN IRDWCISRQL WWGHQIPAYY 

       430        440        450        460        470        480 
CQECGEIVVA RKMPDKCPKC GSTHFKQDED ALDTWFSSAL WPFSTLGWPN KTEALDYYYP 

       490        500        510        520        530        540 
TSVLVTGYDI IFFWVVRMAF AGMFCMNEKP FDHVLVHGLV RDSQGRKMSK SLGNGIDPLE 

       550        560        570        580        590        600 
IIEQYGADAL RFTLTTGNSP ENDMRFYMER VEFARNFANK LWNASRFVFM NIDEDIIKNM 

       610        620        630        640        650        660 
TRESVKEDLT LADKWIISRA NNIVKEATNN MDKFDLGIAL QKIYDFTWSE YCDWYIEMVK 

       670        680        690        700        710        720 
PRLYGEDANA KSAALYTLTY VLEKILKLLH PYMPFITEEI YTHLPTVEGC IIVSEWPKYN 

       730        740        750        760        770        780 
EEDNMAEEED MMNLLMEGIR SIRNVRAEMN VPPSKKAKLI IIPSEEKIEA IELGKDYFIT 

       790        800        810        820        830        840 
LASASNVEIA KDKSNVPEDA VGVVIDGVEI FIPLNELVDF EKEIERLSKE KKKLEGEIKR 

       850        860        870        880 
VNGKLANQGF LAKAPESLIE EEKAKKEKFE EMIKSVEERL TNLESKIK

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM180355 Genomic DNA. Translation: CAJ70153.1.
RefSeqYP_001089772.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ180A1.
ModBaseSearch...

Protein-protein interaction databases

STRING272563.CD3256.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ70153; CAJ70153; CD630_32560.
GeneID4916580.
KEGGcdf:CD630_32560.
PATRIC19445051. VBICloDif38397_3409.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0525.
HOGENOMHOG000020093.
KOK01873.
OMAIMDALIR.
ProtClustDBPRK05729.

Enzyme and pathway databases

BioCycCDIF272563:GJFE-3500-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ180A1_CLOD6
AccessionPrimary (citable) accession number: Q180A1
Entry history
Integrated into UniProtKB/TrEMBL: July 25, 2006
Last sequence update: July 25, 2006
Last modified: May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info