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Protein

GDP-fucose protein O-fucosyltransferase 1

Gene

C15C7.7

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains.1 Publication

Catalytic activityi

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei334Substrate1

GO - Molecular functioni

  • fucosyltransferase activity Source: UniProtKB
  • peptide-O-fucosyltransferase activity Source: WormBase

GO - Biological processi

  • fucose metabolic process Source: UniProtKB-KW
  • Notch signaling pathway Source: UniProtKB-KW
  • protein O-linked fucosylation Source: WormBase
  • protein O-linked glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism, Notch signaling pathway

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BRENDAi2.4.1.221. 1045.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT65. Glycosyltransferase Family 65.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-fucose protein O-fucosyltransferase 1 (EC:2.4.1.221)
Alternative name(s):
Peptide-O-fucosyltransferase 1
Short name:
O-FucT-1
Gene namesi
ORF Names:C15C7.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC15C7.7; CE36786; WBGene00015793.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43N → A: Reduces enzyme activity by over 90%. 1 Publication1
Mutagenesisi240R → A or K: Abolishes enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001215222 – 389GDP-fucose protein O-fucosyltransferase 1Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...)Curated1
Disulfide bondi35 ↔ 371 Publication
Disulfide bondi119 ↔ 1351 Publication
Disulfide bondi249 ↔ 2811 Publication
Disulfide bondi266 ↔ 3531 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ18014.
PaxDbiQ18014.
PeptideAtlasiQ18014.
PRIDEiQ18014.

Expressioni

Gene expression databases

BgeeiWBGene00015793.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi6239.C15C7.7.

Structurei

Secondary structure

1389
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi31 – 34Combined sources4
Beta strandi38 – 40Combined sources3
Helixi41 – 58Combined sources18
Beta strandi61 – 63Combined sources3
Beta strandi67 – 69Combined sources3
Beta strandi71 – 74Combined sources4
Beta strandi76 – 78Combined sources3
Helixi80 – 83Combined sources4
Helixi86 – 89Combined sources4
Turni90 – 92Combined sources3
Helixi98 – 104Combined sources7
Helixi106 – 109Combined sources4
Helixi112 – 114Combined sources3
Beta strandi116 – 121Combined sources6
Helixi143 – 149Combined sources7
Beta strandi156 – 159Combined sources4
Turni164 – 167Combined sources4
Beta strandi170 – 172Combined sources3
Helixi178 – 181Combined sources4
Turni184 – 186Combined sources3
Beta strandi188 – 194Combined sources7
Helixi203 – 211Combined sources9
Helixi216 – 229Combined sources14
Beta strandi232 – 239Combined sources8
Helixi243 – 250Combined sources8
Turni254 – 256Combined sources3
Turni261 – 263Combined sources3
Helixi264 – 267Combined sources4
Helixi269 – 271Combined sources3
Helixi278 – 281Combined sources4
Helixi285 – 299Combined sources15
Beta strandi302 – 310Combined sources9
Helixi314 – 321Combined sources8
Helixi322 – 324Combined sources3
Helixi336 – 345Combined sources10
Beta strandi346 – 351Combined sources6
Helixi356 – 367Combined sources12
Beta strandi368 – 371Combined sources4
Beta strandi375 – 377Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZY2X-ray1.54A26-383[»]
3ZY3X-ray1.86A/B26-383[»]
3ZY4X-ray1.74A26-383[»]
3ZY5X-ray1.96A26-383[»]
3ZY6X-ray1.91A26-383[»]
ProteinModelPortaliQ18014.
SMRiQ18014.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ18014.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 43Substrate binding3
Regioni238 – 240Substrate binding3
Regioni355 – 356Substrate binding2

Sequence similaritiesi

Belongs to the glycosyltransferase 68 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3202. Eukaryota.
KOG3849. Eukaryota.
ENOG410Y3JQ. LUCA.
GeneTreeiENSGT00390000015634.
HOGENOMiHOG000231651.
InParanoidiQ18014.
KOiK03691.
OMAiQSNHFIG.
OrthoDBiEOG091G048B.
PhylomeDBiQ18014.

Family and domain databases

InterProiIPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view]
PfamiPF10250. O-FucT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q18014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVSKVLTLA SFISVCSYSE AKSNETDPNG YIVFCPCMGR FGNQVDQFLG
60 70 80 90 100
VLAFAKALDR TLVLPNFIEF KHPETKMIPF EFLFQVGTVA KYTRVVTMQE
110 120 130 140 150
FTKKIMPTVW PPEKRKAFCW TPRQAIYDKS AEPGCHSKEG NPFGPYWDQI
160 170 180 190 200
DVSFVGDEYF GDIPGGFDLN QMGSRKKWLE KFPSEEYPVL AFSSAPAPFP
210 220 230 240 250
SKGKVWSIQK YLRWSSRITE QAKKFISANL AKPFVAVHLR NDADWVRVCE
260 270 280 290 300
HIDTTTNRPL FASEQCLGEG HHLGTLTKEI CSPSKQQILE QIVEKVGSIG
310 320 330 340 350
AKSVFVASDK DHMIDEINEA LKPYEIEAHR QEPDDMYTSL AIMGRADLFV
360 370 380
GNCVSTFSHI VKRERDHAGQ SPRPSAFFGI RAVKRHIDL
Length:389
Mass (Da):44,051
Last modified:February 1, 2005 - v5
Checksum:i0C84F202AA184554
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080550 Genomic DNA. Translation: CCD64591.1.
RefSeqiNP_741744.2. NM_171649.6.
UniGeneiCel.30602.

Genome annotation databases

EnsemblMetazoaiC15C7.7; C15C7.7; WBGene00015793.
GeneIDi180607.
KEGGicel:CELE_C15C7.7.
UCSCiC15C7.7. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080550 Genomic DNA. Translation: CCD64591.1.
RefSeqiNP_741744.2. NM_171649.6.
UniGeneiCel.30602.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZY2X-ray1.54A26-383[»]
3ZY3X-ray1.86A/B26-383[»]
3ZY4X-ray1.74A26-383[»]
3ZY5X-ray1.96A26-383[»]
3ZY6X-ray1.91A26-383[»]
ProteinModelPortaliQ18014.
SMRiQ18014.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C15C7.7.

Protein family/group databases

CAZyiGT65. Glycosyltransferase Family 65.

Proteomic databases

EPDiQ18014.
PaxDbiQ18014.
PeptideAtlasiQ18014.
PRIDEiQ18014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC15C7.7; C15C7.7; WBGene00015793.
GeneIDi180607.
KEGGicel:CELE_C15C7.7.
UCSCiC15C7.7. c. elegans.

Organism-specific databases

CTDi180607.
WormBaseiC15C7.7; CE36786; WBGene00015793.

Phylogenomic databases

eggNOGiKOG3202. Eukaryota.
KOG3849. Eukaryota.
ENOG410Y3JQ. LUCA.
GeneTreeiENSGT00390000015634.
HOGENOMiHOG000231651.
InParanoidiQ18014.
KOiK03691.
OMAiQSNHFIG.
OrthoDBiEOG091G048B.
PhylomeDBiQ18014.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.221. 1045.

Miscellaneous databases

EvolutionaryTraceiQ18014.
PROiQ18014.

Gene expression databases

BgeeiWBGene00015793.

Family and domain databases

InterProiIPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view]
PfamiPF10250. O-FucT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOFUT1_CAEEL
AccessioniPrimary (citable) accession number: Q18014
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: February 1, 2005
Last modified: November 30, 2016
This is version 104 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

Manganese is bound to the substrate GDP-fucose, but is not required for enzyme activity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.