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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Helicobacter acinonychis (strain Sheeba)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Co2+UniRule annotationNote: Binds 2 divalent ions per subunit. Magnesium or cobalt.UniRule annotation
  • K(+)UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi18 – 181MagnesiumUniRule annotation
Metal bindingi44 – 441PotassiumUniRule annotation
Metal bindingi264 – 2641PotassiumUniRule annotation
Metal bindingi272 – 2721MagnesiumUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi260 – 2678ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. methionine adenosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. one-carbon metabolic process Source: UniProtKB-HAMAP
  2. S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciHACI382638:GJAU-361-MONOMER.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:Hac_0382
OrganismiHelicobacter acinonychis (strain Sheeba)
Taxonomic identifieri382638 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000775: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385S-adenosylmethionine synthasePRO_0000302922Add
BLAST

Proteomic databases

PRIDEiQ17YQ7.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi382638.Hac_0382.

Structurei

3D structure databases

ProteinModelPortaliQ17YQ7.
SMRiQ17YQ7. Positions 6-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0192.
HOGENOMiHOG000245710.
KOiK00789.
OMAiIGEQSPD.
OrthoDBiEOG68WR6M.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17YQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDSFLFTSE SVTEGHPDKM ADQISDAVLD YIIERDKKAK VACETLVSNG
60 70 80 90 100
FCVITGELKT SIYAPMQEIA REVVKKIGYT DALYGFDYRS AAVLNGIGEQ
110 120 130 140 150
SPDINQGVDR EDGEIGAGDQ GLVFGYACKE TQMLMPLPIH LAHQLTFALA
160 170 180 190 200
QKRKDNTLPF LRPDGKSQVS VRYENNKPIS IDTIVISTQH SPEVSQKHLK
210 220 230 240 250
EAVIEEIVYK VLPKEYLHDN IKFFVNPTGK FVIGGPQGDA GLTGRKIIVD
260 270 280 290 300
TYGGSCPHGG GAFSGKDPSK VDRSAAYAAR YVAKNLVASG VCDRATVQLA
310 320 330 340 350
YAIGVVEPVS IYVNTHNTSK YSSAELEKCV KLVFKLTPKG IIESLDLLRP
360 370 380
IYSLTSSYGH FGRELEAFTW EKTNKAEEIK AFFKH
Length:385
Mass (Da):42,358
Last modified:July 25, 2006 - v1
Checksum:iFA68A27F9790647A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260522 Genomic DNA. Translation: CAJ99219.1.
RefSeqiYP_664218.1. NC_008229.1.

Genome annotation databases

EnsemblBacteriaiCAJ99219; CAJ99219; Hac_0382.
GeneIDi4176762.
KEGGihac:Hac_0382.
PATRICi20584697. VBIHelAci71660_0374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260522 Genomic DNA. Translation: CAJ99219.1.
RefSeqiYP_664218.1. NC_008229.1.

3D structure databases

ProteinModelPortaliQ17YQ7.
SMRiQ17YQ7. Positions 6-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi382638.Hac_0382.

Proteomic databases

PRIDEiQ17YQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ99219; CAJ99219; Hac_0382.
GeneIDi4176762.
KEGGihac:Hac_0382.
PATRICi20584697. VBIHelAci71660_0374.

Phylogenomic databases

eggNOGiCOG0192.
HOGENOMiHOG000245710.
KOiK00789.
OMAiIGEQSPD.
OrthoDBiEOG68WR6M.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
BioCyciHACI382638:GJAU-361-MONOMER.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines."
    Eppinger M., Baar C., Linz B., Raddatz G., Lanz C., Keller H., Morelli G., Gressmann H., Achtman M., Schuster S.C.
    PLoS Genet. 2:1097-1110(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sheeba.

Entry informationi

Entry nameiMETK_HELAH
AccessioniPrimary (citable) accession number: Q17YQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 25, 2006
Last modified: March 4, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.