Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q17YQ7 (METK_HELAH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-adenosylmethionine synthase

Short name=AdoMet synthase
EC=2.5.1.6
Alternative name(s):
MAT
Methionine adenosyltransferase
Gene names
Name:metK
Ordered Locus Names:Hac_0382
OrganismHelicobacter acinonychis (strain Sheeba) [Complete proteome] [HAMAP]
Taxonomic identifier382638 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. HAMAP-Rule MF_00086

Catalytic activity

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP-Rule MF_00086

Cofactor

Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity.

Binds 1 potassium ion per subunit By similarity. HAMAP-Rule MF_00086

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP-Rule MF_00086

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00086

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00086.

Sequence similarities

Belongs to the AdoMet synthase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   Cellular componentCytoplasm
   LigandATP-binding
Cobalt
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processS-adenosylmethionine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

one-carbon metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methionine adenosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385S-adenosylmethionine synthase HAMAP-Rule MF_00086
PRO_0000302922

Regions

Nucleotide binding260 – 2678ATP Potential

Sites

Metal binding181Magnesium By similarity
Metal binding441Potassium By similarity
Metal binding2641Potassium By similarity
Metal binding2721Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q17YQ7 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: FA68A27F9790647A

FASTA38542,358
        10         20         30         40         50         60 
MKDSFLFTSE SVTEGHPDKM ADQISDAVLD YIIERDKKAK VACETLVSNG FCVITGELKT 

        70         80         90        100        110        120 
SIYAPMQEIA REVVKKIGYT DALYGFDYRS AAVLNGIGEQ SPDINQGVDR EDGEIGAGDQ 

       130        140        150        160        170        180 
GLVFGYACKE TQMLMPLPIH LAHQLTFALA QKRKDNTLPF LRPDGKSQVS VRYENNKPIS 

       190        200        210        220        230        240 
IDTIVISTQH SPEVSQKHLK EAVIEEIVYK VLPKEYLHDN IKFFVNPTGK FVIGGPQGDA 

       250        260        270        280        290        300 
GLTGRKIIVD TYGGSCPHGG GAFSGKDPSK VDRSAAYAAR YVAKNLVASG VCDRATVQLA 

       310        320        330        340        350        360 
YAIGVVEPVS IYVNTHNTSK YSSAELEKCV KLVFKLTPKG IIESLDLLRP IYSLTSSYGH 

       370        380 
FGRELEAFTW EKTNKAEEIK AFFKH 

« Hide

References

[1]"Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines."
Eppinger M., Baar C., Linz B., Raddatz G., Lanz C., Keller H., Morelli G., Gressmann H., Achtman M., Schuster S.C.
PLoS Genet. 2:1097-1110(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sheeba.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260522 Genomic DNA. Translation: CAJ99219.1.
RefSeqYP_664218.1. NC_008229.1.

3D structure databases

ProteinModelPortalQ17YQ7.
SMRQ17YQ7. Positions 6-380.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING382638.Hac_0382.

Proteomic databases

PRIDEQ17YQ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ99219; CAJ99219; Hac_0382.
GeneID4176762.
KEGGhac:Hac_0382.
PATRIC20584697. VBIHelAci71660_0374.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0192.
HOGENOMHOG000245710.
KOK00789.
OMAIYYAHRL.
OrthoDBEOG68WR6M.
ProtClustDBPRK05250.

Enzyme and pathway databases

BioCycHACI382638:GJAU-361-MONOMER.
UniPathwayUPA00315; UER00080.

Family and domain databases

HAMAPMF_00086. S_AdoMet_synth1.
InterProIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERPTHR11964. PTHR11964. 1 hit.
PfamPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000497. MAT. 1 hit.
SUPFAMSSF55973. SSF55973. 3 hits.
TIGRFAMsTIGR01034. metK. 1 hit.
PROSITEPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMETK_HELAH
AccessionPrimary (citable) accession number: Q17YQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 25, 2006
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways