Reviewed, UniProtKB/Swiss-Prot Q17X65 (NADE_HELAH)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Hac_0991

Organism

Helicobacter acinonychis (strain Sheeba) [Complete proteome] [HAMAP]

Taxonomic identifier

382638 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077559
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
Sites
	Active site	33	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q17X65-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 0FDCBBD00A3C8BC3	FASTA	260	29,354
	10 20 30 40 50 60 MQKHYHKLIS YLCDFLEKET QKRGFKKVVY GLSGGLDSAV VGVLSQKVFK ENAHALLMPS 70 80 90 100 110 120 LVSMPESKSD ALDLCETFSI PYTEYSIAPY DAIFCSYFKD ASLTRKGNFC SRLRMAFLYD 130 140 150 160 170 180 YSLKSNSLVI GTSNKSERML GYGTLFGDLA CAINPIGELF KTEVYELAQH LNIPKKILDK 190 200 210 220 230 240 PPSADLFVGQ SDEKDLGYPY SVIDPLLKDI EALFRTKPIH LETLTQLGYD ETLVKNTISR 250 260 IQKNAFKLEL PTIAKRFDPK

References

[1]

"Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines." Eppinger M., Baar C., Linz B., Raddatz G., Lanz C., Keller H., Morelli G., Gressmann H., Achtman M., Schuster S.C. PLoS Genet. 2:1097-1110(2006) [PubMed: 16789826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM260522 Genomic DNA. Translation: CAJ99761.1.
RefSeq	YP_664760.1.
3D structure databases
SMR	Q17X65. Positions 3-260.
ModBase	Search...
Protein-protein interaction databases
STRING	Q17X65.
Genome annotation databases
GeneID	4177665.
GenomeReviews	Gene locus Hac_0991 in contig AM260522_GR.
KEGG	hac:Hac_0991.
NMPDR	fig|382638.8.peg.963.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q17X65.
OMA	CAINPIG.
Enzyme and pathway databases
BioCyc	HACI382638:HAC_0991-MON.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_HELAH

Accession

Primary (citable) accession number: Q17X65

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 25, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)