Reviewed, UniProtKB/Swiss-Prot Q17W17 (PYRC_HELAH)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Hac_1431

Organism

Helicobacter acinonychis (strain Sheeba) [Complete proteome] [HAMAP]

Taxonomic identifier

382638 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Dihydroorotase		PRO_1000024016
Sites
	Metal binding	12	1	Zinc 1 By similarity
	Metal binding	14	1	Zinc 1 By similarity
	Metal binding	94	1	Zinc 1; via carbamate group By similarity
	Metal binding	94	1	Zinc 2; via carbamate group By similarity
	Metal binding	133	1	Zinc 2 By similarity
	Metal binding	167	1	Zinc 2 By similarity
	Metal binding	239	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	94	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q17W17-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: F40860B3AF155A31	FASTA	339	38,001
	10 20 30 40 50 60 MEITLFDPID AHLHVREGVL LKAVLKYSSE PFSAAVIMPN LSKPLIDTQI TLEYEGEILK 70 80 90 100 110 120 NSSNFKPLMS LYFNDGLTLE ELQHAKHQGI KFLKLYPKGM TTNAQNGTSD LLGEKTLEIL 130 140 150 160 170 180 EDAQKLGFIL CIHAEQAGFC LDKEFLCHSV LETFAHSFPK LKIIIEHLSD WRSIALIEKH 190 200 210 220 230 240 DNLYATLTLH HISMTLDDLL GGSLNPHCFC KPLIKTQKDQ ERLLSLALKA HPKISFGSDS 250 260 270 280 290 300 APHVISKKHS ANIPAGIFSA PILLPALCEL FEKHNALENL QAFISDNAKK IYTLDNLPSK 310 320 330 KVRLSKKPFI VPTHTLCLNE KIAILREGET LSWNIQEIA

References

[1]

"Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines." Eppinger M., Baar C., Linz B., Raddatz G., Lanz C., Keller H., Morelli G., Gressmann H., Achtman M., Schuster S.C. PLoS Genet. 2:1097-1110(2006) [PubMed: 16789826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM260522 Genomic DNA. Translation: CAK00159.1.
RefSeq	YP_665158.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4177751.
GenomeReviews	Gene locus Hac_1431 in contig AM260522_GR.
KEGG	hac:Hac_1431.
NMPDR	fig|382638.8.peg.1385.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q17W17.
Enzyme and pathway databases
BioCyc	HACI382638:HAC_1431-MON.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_HELAH

Accession

Primary (citable) accession number: Q17W17

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 25, 2006
Last modified:	November 25, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)