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Q17W06 (AMPA_HELAH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase

EC=3.4.11.1
Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase
Gene names
Name:pepA
Ordered Locus Names:Hac_1442
OrganismHelicobacter acinonychis (strain Sheeba) [Complete proteome] [HAMAP]
Taxonomic identifier382638 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP-Rule MF_00181

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00181.

Sequence similarities

Belongs to the peptidase M17 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandManganese
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloexopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Probable cytosol aminopeptidase HAMAP-Rule MF_00181
PRO_1000019926

Sites

Active site2701 Potential
Active site3441 Potential
Metal binding2581Manganese 2 By similarity
Metal binding2631Manganese 1 By similarity
Metal binding2631Manganese 2 By similarity
Metal binding2811Manganese 2 By similarity
Metal binding3401Manganese 1 By similarity
Metal binding3421Manganese 1 By similarity
Metal binding3421Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q17W06 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: CDCC18C244A6B076

FASTA49654,522
        10         20         30         40         50         60 
MLKIKLEKSA FENAKAECSL VFIVNKDFNH AWVKNKKLLE TFRYEGEDSF LDQENKILYV 

        70         80         90        100        110        120 
GVKEDNVHLL RESACSAIRT LKKLAFKSVK VGVYTCHTHS KDNALLENLK ALFLGLKLGM 

       130        140        150        160        170        180 
YEYDTFKSNK KESVLKEAII ALELHKPCEE TCANSLEKSA KEALKYADVM TESLNITKNL 

       190        200        210        220        230        240 
VNTPPMIGTP IYMAEVAQKV AKEGNLEIHV YDEKFLEEKK MNAFLAVNKA SLGVNPPRLI 

       250        260        270        280        290        300 
HLIYKPKKAK KKIALVGKGL TYDCGGLSLK PADYMVTMKA DKGGGSAVIG LLNALSNLGV 

       310        320        330        340        350        360 
EAEVHGIIGA TENMIGPAAY KPDDILISKE GKSIEVRNTD AEGRLVLADC LSYAQDLNPD 

       370        380        390        400        410        420 
VIVDFATLTG ACVVGLGEFT SAIMGHNEEL KNLFETSGLE SGELLAKLPF NRHLKKLIES 

       430        440        450        460        470        480 
KIADVCNVSA SRYGGAITAG LFLNEFIRDE FKDKWLHIDI AGPAYVEKEW DVNSFGASGA 

       490 
GVRACTAFVE ELLKKA 

« Hide

References

[1]"Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines."
Eppinger M., Baar C., Linz B., Raddatz G., Lanz C., Keller H., Morelli G., Gressmann H., Achtman M., Schuster S.C.
PLoS Genet. 2:1097-1110(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sheeba.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260522 Genomic DNA. Translation: CAK00170.1.
RefSeqYP_665169.1. NC_008229.1.

3D structure databases

ProteinModelPortalQ17W06.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING382638.Hac_1442.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK00170; CAK00170; Hac_1442.
GeneID4176744.
KEGGhac:Hac_1442.
PATRIC20586722. VBIHelAci71660_1365.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0260.
HOGENOMHOG000243129.
KOK01255.
OMAADYMVTM.
OrthoDBEOG6FV8B3.
ProtClustDBPRK00913.

Enzyme and pathway databases

BioCycHACI382638:GJAU-1335-MONOMER.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
InterProIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERPTHR11963:SF3. PTHR11963:SF3. 1 hit.
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPA_HELAH
AccessionPrimary (citable) accession number: Q17W06
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries