Q17VI1 (CARA_HELAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	Hac_1638

Organism

Helicobacter acinonychis (strain Sheeba) [Complete proteome] [HAMAP]

Taxonomic identifier

382638 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_1000138861
Regions
	Domain	188 – 378	191	Glutamine amidotransferase type-1
	Region	1 – 184	184	CPSase HAMAP MF_01209
Sites
	Active site	268	1	Nucleophile By similarity
	Active site	351	1	By similarity
	Active site	353	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q17VI1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: C1A7B0D99B1428F5	FASTA	379	42,246
	10 20 30 40 50 60 MVSLYLENGL FLQVQSFGAS GTQVGELVFN TSMSGYQEVI SDPSYKGQFV IFSMPEIGVV 70 80 90 100 110 120 GANSKDDESF FSSAGILVRH YNEFFSNSRA DFSLSAYLKE RGVLGICGVD TRSLIKTLRH 130 140 150 160 170 180 HGCLMMVAST IEHDKNKLEE ILKNAPRISH TPLVASVSTP KIITHQRATF DFKTLDYKPF 190 200 210 220 230 240 DEKNSHKTIA VLDFGAKGNI LNELQNVGLK ALIYPHHTKA NELIKAYEKK EIHGIFLSNG 250 260 270 280 290 300 PGDPLSLQQE IGEIKQLIGA KIPMFGICLG HQLLSIAQGY PTYKLKFGHH GSNHPVKNLE 310 320 330 340 350 360 TNAVEITAQN HNYCVPEAIE KIATITHRNL FDNTIEGVHY KNAPIISVQH HPESSPGPKE 370 SHYIFKEFVK LLENFPTRE

References

[1]

"Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines." Eppinger M., Baar C., Linz B., Raddatz G., Lanz C., Keller H., Morelli G., Gressmann H., Achtman M., Schuster S.C. PLoS Genet. 2:1097-1110(2006) [PubMed: 16789826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sheeba.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260522 Genomic DNA. Translation: CAK00345.1.
RefSeq	YP_665344.1. NC_008229.1.
3D structure databases
ProteinModelPortal	Q17VI1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q17VI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4177748.
GenomeReviews	Gene locus Hac_1638 in contig AM260522_GR.
KEGG	hac:Hac_1638.
NMPDR	fig|382638.8.peg.1585.
PATRIC	20587110. VBIHelAci71660_1550.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0505.
HOGENOM	HBG286341.
OMA	FTYPELG.
ProtClustDB	PRK12564.
Enzyme and pathway databases
BioCyc	HACI382638:HAC_1638-MONOMER.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_HELAH

Accession

Primary (citable) accession number: Q17VI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program