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Q17VA0 (BIOB_HELAH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Hac_1730
OrganismHelicobacter acinonychis (strain Sheeba) [Complete proteome] [HAMAP]
Taxonomic identifier382638 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282Biotin synthase HAMAP-Rule MF_01694
PRO_0000381422

Sites

Metal binding171Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding211Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding241Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1541Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2211Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q17VA0 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: C833E46C3237538D

FASTA28231,459
        10         20         30         40         50         60 
MQEIFLCSIS NVRSGDCKED CAYCTQSSHH QGAIKRYKFK DEKVVLQEAR ALRELGALGF 

        70         80         90        100        110        120 
CLVTSGRELD DEKCEYIAKL AKAINQEELG LHLIACCGRA DLDQLEFLRD AGIHSYNHNL 

       130        140        150        160        170        180 
ETSQNFFPKI CSTHTWEERF ITCENALRAG LGLCSGGIFG LNESWEDRIE MLRALASLSP 

       190        200        210        220        230        240 
HTTPINFFIK NPVLPIDAET LSADEALECV LLAKEFLPNA RLMAAGGREV VFKDNDKKEA 

       250        260        270        280 
KLFEYGINAV VLGDYLTTKG KAPKKDIERL LSYGLKMAAS CH 

« Hide

References

[1]"Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines."
Eppinger M., Baar C., Linz B., Raddatz G., Lanz C., Keller H., Morelli G., Gressmann H., Achtman M., Schuster S.C.
PLoS Genet. 2:1097-1110(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sheeba.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260522 Genomic DNA. Translation: CAK00426.1.
RefSeqYP_665425.1. NC_008229.1.

3D structure databases

ProteinModelPortalQ17VA0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING382638.Hac_1730.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK00426; CAK00426; Hac_1730.
GeneID4177114.
KEGGhac:Hac_1730.
PATRIC20587282. VBIHelAci71660_1636.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMAWEERFIT.
OrthoDBEOG622PMP.
ProtClustDBPRK08508.

Enzyme and pathway databases

BioCycHACI382638:GJAU-1600-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_HELAH
AccessionPrimary (citable) accession number: Q17VA0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 25, 2006
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways