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Reviewed, UniProtKB/Swiss-Prot Q17R13 (ACK1_BOVIN)

Last modified February 9, 2010. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Activated CDC42 kinase 1
      Short name=ACK-1
    EC=2.7.10.2
Alternative name(s):
    Tyrosine kinase non-receptor protein 2
    Activated CDC42 kinase 2
Gene names
Name: TNK2
Synonyms: ACK1, ACK2
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length1039 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development By similarity. UniProtKB Q07912

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. UniProtKB Q07912

Cofactor

Magnesium By similarity. UniProtKB Q07912

Enzyme regulation

The SH3 domain appears to play an autoinhibitory role By similarity. UniProtKB O54967

Subunit structure

Interacts with CDC42. Interacts with activated CSPG4 By similarity. Interacts with AR By similarity. Ref.1 UniProtKB Q07912

Subcellular location

Cell membrane By similarity UniProtKB O54967.

Tissue specificity

Expressed in brain and skeletal muscle. Weakly expressed in pancreas, heart, placenta and lung.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family.

Contains 1 protein kinase domain.

Contains 1 SH3 domain.

Contains 1 UBA domain.

Sequence caution

The sequence AAC05310.1 differs from that shown. Reason: Frameshift at position 682.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDC42P609531EBI-457220,EBI-81752From a different organism.
CLTCQ006101EBI-457220,EBI-354967From a different organism.
SNX9Q9Y5X12EBI-457220,EBI-77848From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q17R13-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q17R13-2)

The sequence of this isoform differs from the canonical sequence as follows:
     514-514: K → KREPPPRPPQPAIFTQ
     814-856: MPTTQSFASD...VRDGKKVSST → ENYVQNFKNL...SPECGRETPF
     857-1039: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10391039Activated CDC42 kinase 1
PRO_0000312829

Regions

Domain126 – 385260Protein kinase
Domain373 – 44876SH3
Domain957 – 99741UBA
Nucleotide binding132 – 1409ATP By similarity UniProtKB P00517
Compositional bias576 – 918343Pro-rich

Sites

Active site2521Proton acceptor By similarity UniProtKB P00517
Binding site1581ATP By similarity UniProtKB O54967

Amino acid modifications

Modified residue1011Phosphothreonine By similarity
Modified residue1491Phosphoserine By similarity UniProtKB Q07912
Modified residue2841Phosphotyrosine; by autocatalysis By similarity UniProtKB Q07912
Modified residue5171Phosphothreonine By similarity
Modified residue5181Phosphotyrosine By similarity UniProtKB Q07912
Modified residue7231Phosphoserine By similarity
Modified residue7271Phosphoserine By similarity
Modified residue7841Phosphoserine By similarity
Modified residue8261Phosphotyrosine By similarity UniProtKB Q07912
Modified residue8581Phosphotyrosine By similarity UniProtKB Q07912
Modified residue8591Phosphotyrosine By similarity UniProtKB Q07912
Modified residue8801Phosphoserine By similarity

Natural variations

Alternative sequence5141K → KREPPPRPPQPAIFTQ in isoform 2.
VSP_038165
Alternative sequence814 – 85643MPTTQ…KVSST → ENYVQNFKNLTAHHPPWRDQ DTGTGSSRGPTVLSPECGRE TPF in isoform 2.
VSP_038166
Alternative sequence857 – 1039183Missing in isoform 2.
VSP_038167

Experimental info

Sequence conflict2031T → M in AAC05310. Ref.1
Sequence conflict5341R → K in AAC05310. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: E7834771D76F8472

FASTA1,039114,868
        10         20         30         40         50         60 
MQPEEGTGWL LELLSEVQLQ QYFLRLRDDL NVTRLSHFEY VKNEDLEKIG MGRPGQRRLW 

        70         80         90        100        110        120 
EAVKRRKAMC KRKSWMSKVF SGKRLEAEFP PHHSQSTFRK TSPTPGGSAG EGSLQSLTCL 

       130        140        150        160        170        180 
IGEKDLHLFE KLGDGSFGVV RRGEWDAPSG KTVSVAVKCL KPDVLSQPEA MDDFIREVNA 

       190        200        210        220        230        240 
MHSLDHRNLI RLYGVVLTPP MKTVTELAPL GSLLDRLRKH QGHFLLGTLS RYAVQVAEGM 

       250        260        270        280        290        300 
GYLEAKRFIH RDLAARNLLL ATRDLVKIGD FGLMRALPQN DDHYVMQEHR KVPFAWCAPE 

       310        320        330        340        350        360 
SLKTRTFSHA SDTWMFGVTL WEMFTYGQEP WIGLNGSQIL HKIDKEGERL PRPEDCPQDI 

       370        380        390        400        410        420 
YNVMVQCWAH KPEDRPTFVA LRDFLLEAQP TDMRALQDFE EPDKLHIQMN DVITVIEGRA 

       430        440        450        460        470        480 
ENYWWRGQNT RTLCVGPFPR NVVTSVAGLS AQDISQPLQN SFIHTGHGDS DPRHCWGFPD 

       490        500        510        520        530        540 
KIDELYLGNP MDPPDLLSVE LSTSRPTQHL GRVKKPTYDP VSEDQDPLSS DFKRLGLRKP 

       550        560        570        580        590        600 
GLPRGLWLAK PSARVPGTKA GRGGGEVTLI DFGEEPVVPA PRPCAPSLAQ LAMDACSLLD 

       610        620        630        640        650        660 
KTPPQSPTRA LPRPLHPTPV VDWDARPLPP PPAYDDVAQD EDDFEVCSIN STLVGAGVSA 

       670        680        690        700        710        720 
EPSQGETNYA FVPEPARLLP PLEDNLFLPP QSGGKPPNSA QTAEIFQALQ QECMRQLQVP 

       730        740        750        760        770        780 
PGSLVPSPSP GGDDKPQVPP RVPIPPRPTR SRGELSPVPP GEEEMGRWPG PASPPRVPPR 

       790        800        810        820        830        840 
EPLSPQGSRT PSPLVPPGSS PLPPRLSSSP GKTMPTTQSF ASDPKYATPQ VIQAPGPRAG 

       850        860        870        880        890        900 
PCILPIVRDG KKVSSTHYYL LPERPPYLER YQRFLHEAQS PRGPDPTPIP LLLPPPSTPA 

       910        920        930        940        950        960 
PAAPTATVRP MPQAAPDPKA NFSSNNSNPG ARPSSLRATA RLPQRGYPGD GPEAGRPADK 

       970        980        990       1000       1010       1020 
IQMLQAMVHG VTTEECQAAL QSHSWSVQRA AQYLKVEQLF GLGLRPRGEC HNVLEMFDWN 

      1030 
LEQAGCHLLG SCGPAHHKR 

« Hide

Isoform 2.

Checksum: 3BE6448F4370A6E1
Show »

FASTA87196,809

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel Cdc42-associated tyrosine kinase, ACK-2, from bovine brain."
Yang W., Cerione R.A.
J. Biol. Chem. 272:24819-24824(1997) [PubMed: 9312079] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CDC42.
Tissue: Brain.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Hereford.
Tissue: Hypothalamus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U96722 mRNA. Translation: AAC05310.1. Frameshift.
BC118081 mRNA. Translation: AAI18082.1.
IPIIPI00700705.
IPI00944890.
RefSeqNP_776310.2.
UniGeneBt.64988

3D structure databases

SMRQ17R13. Positions 117-388, 392-444, 447-489.
ModBaseSearch...

Protein-protein interaction databases

IntActQ17R13. 4 interactions.
STRINGQ17R13.

Genome annotation databases

EnsemblENSBTAT00000028482; ENSBTAP00000028482; ENSBTAG00000021364; Bos taurus. [Genome view]
ENSBTAT00000046471; ENSBTAP00000043771; ENSBTAG00000021364; Bos taurus. [Genome view]
GeneID280710.
KEGGbta:280710.

Organism-specific databases

CTD280710.

Phylogenomic databases

HOVERGENQ17R13.
InParanoidQ17R13.

Enzyme and pathway databases

BRENDA2.7.10.2. 251.

Family and domain databases

InterProIPR015116. GTPase_binding.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001452. SH3_domain.
IPR015779. Tyr_kinase_non-rcpt_Ack_N.
IPR020635. Tyr_Pkinase_cat_dom.
IPR020685. Tyr_prot_kinase.
IPR008266. Tyr_prot_kinase_AS.
IPR000449. UBA/transl_elong_EF1B_N.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
PANTHERPTHR23256:SF265. ACK_tyr_kin_N. 1 hit.
PTHR23256. Tyr_prot_kinase. 1 hit.
PfamPF09027. GTPase_binding. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50002. SH3. 1 hit.
PS50030. UBA. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACK1_BOVIN
AccessionPrimary (citable) accession number: Q17R13
Secondary accession number(s): O02742
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: July 25, 2006
Last modified: February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents