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Protein

Target of rapamycin complex subunit LST8

Gene

MLST8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657' (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-BTA-1257604. PIP3 activates AKT signaling.
R-BTA-1632852. Macroautophagy.
R-BTA-165159. mTOR signalling.
R-BTA-166208. mTORC1-mediated signalling.
R-BTA-3371571. HSF1-dependent transactivation.
R-BTA-380972. Energy dependent regulation of mTOR by LKB1-AMPK.
R-BTA-389357. CD28 dependent PI3K/Akt signaling.
R-BTA-5218920. VEGFR2 mediated vascular permeability.
R-BTA-5628897. TP53 Regulates Metabolic Genes.
R-BTA-6804757. Regulation of TP53 Degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex subunit LST8
Short name:
TORC subunit LST8
Alternative name(s):
G protein beta subunit-like
Short name:
Protein GbetaL
Mammalian lethal with SEC13 protein 8
Short name:
mLST8
Gene namesi
Name:MLST8
Synonyms:GBL, LST8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003264981 – 326Target of rapamycin complex subunit LST8Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ17QU5.
PRIDEiQ17QU5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000009928.
ExpressionAtlasiQ17QU5. differential.

Interactioni

Subunit structurei

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR (By similarity). mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR (By similarity). Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts directly with MTOR and RPTOR (By similarity). Interacts with RHEB (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013104.

Structurei

3D structure databases

ProteinModelPortaliQ17QU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 37WD 1Add BLAST37
Repeati40 – 80WD 2Add BLAST41
Repeati83 – 122WD 3Add BLAST40
Repeati126 – 165WD 4Add BLAST40
Repeati168 – 207WD 5Add BLAST40
Repeati218 – 257WD 6Add BLAST40
Repeati268 – 309WD 7Add BLAST42

Sequence similaritiesi

Belongs to the WD repeat LST8 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0315. Eukaryota.
ENOG410XPVD. LUCA.
GeneTreeiENSGT00390000014795.
HOGENOMiHOG000208395.
HOVERGENiHBG054763.
InParanoidiQ17QU5.
KOiK08266.
TreeFamiTF318577.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17QU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSPGTVGS DPVILATAGY DHTVRFWQAH SGICTRTVQH QDSQVNALEI
60 70 80 90 100
TPDRTMIAAA GYQHIRMYDL NSNNPNPIIS YDGVNKNVAS VGFHEDGRWM
110 120 130 140 150
YTGGEDCTAR IWDLRSRNLQ CQRIFQVNAP INCVCLHPNQ AELIVGDQSG
160 170 180 190 200
AIHIWDLKTD HNEQLIPEPE VSITSAHIDP DASYMAAVNS TGNCYVWNLT
210 220 230 240 250
GGIGDEVTQL IPKTKIPAHT RYALQCRFSP DSTLLATCSA DQTCKIWRTS
260 270 280 290 300
NFSLMTELSI KSSNPGESSR GWMWGCAFSG DSQYIVTASS DNLARLWCVE
310 320
TGEIKREYGG HQKAVVCLAF NDSVLG
Length:326
Mass (Da):35,920
Last modified:July 25, 2006 - v1
Checksum:iD3FDCDF6EC03E541
GO

Sequence cautioni

The sequence AAX46409 differs from that shown. Reason: Frameshift at position 321.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021562 mRNA. Translation: AAX46409.1. Frameshift.
BC118176 mRNA. Translation: AAI18177.1.
RefSeqiNP_001030488.1. NM_001035411.1.
XP_005224583.1. XM_005224526.3.
UniGeneiBt.3376.

Genome annotation databases

EnsembliENSBTAT00000053181; ENSBTAP00000048491; ENSBTAG00000009928.
GeneIDi535236.
KEGGibta:535236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021562 mRNA. Translation: AAX46409.1. Frameshift.
BC118176 mRNA. Translation: AAI18177.1.
RefSeqiNP_001030488.1. NM_001035411.1.
XP_005224583.1. XM_005224526.3.
UniGeneiBt.3376.

3D structure databases

ProteinModelPortaliQ17QU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013104.

Proteomic databases

PaxDbiQ17QU5.
PRIDEiQ17QU5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000053181; ENSBTAP00000048491; ENSBTAG00000009928.
GeneIDi535236.
KEGGibta:535236.

Organism-specific databases

CTDi64223.

Phylogenomic databases

eggNOGiKOG0315. Eukaryota.
ENOG410XPVD. LUCA.
GeneTreeiENSGT00390000014795.
HOGENOMiHOG000208395.
HOVERGENiHBG054763.
InParanoidiQ17QU5.
KOiK08266.
TreeFamiTF318577.

Enzyme and pathway databases

ReactomeiR-BTA-1257604. PIP3 activates AKT signaling.
R-BTA-1632852. Macroautophagy.
R-BTA-165159. mTOR signalling.
R-BTA-166208. mTORC1-mediated signalling.
R-BTA-3371571. HSF1-dependent transactivation.
R-BTA-380972. Energy dependent regulation of mTOR by LKB1-AMPK.
R-BTA-389357. CD28 dependent PI3K/Akt signaling.
R-BTA-5218920. VEGFR2 mediated vascular permeability.
R-BTA-5628897. TP53 Regulates Metabolic Genes.
R-BTA-6804757. Regulation of TP53 Degradation.

Gene expression databases

BgeeiENSBTAG00000009928.
ExpressionAtlasiQ17QU5. differential.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLST8_BOVIN
AccessioniPrimary (citable) accession number: Q17QU5
Secondary accession number(s): Q58DN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 25, 2006
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.