Q17QS4 (PI5L1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-phosphate 5-kinase-like protein 1
Short name=PI(4)P 5-kinase-like protein 1
Short name=PtdIns(4)P-5-kinase-like protein 1
EC=2.7.1.68

Gene names

Name:

PIP5KL1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May act as a scaffold to localize and regulate type I PI4P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3 By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Subunit structure	Heterodimerizes with other type I phosphatidylinositol 4-phosphate 5-kinase By similarity.
Subcellular location	Cytoplasm. Membrane. Note: Localized to large cytoplasmic vesicular structures By similarity.
Sequence similarities	Contains 1 PIPK domain.
Caution	It is unsure if the enzyme has intrinsic kinase activity.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol phosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-phosphatidylinositol-4-phosphate 5-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q17QS4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q17QS4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-224: Missing.
     225-257: CEVSRWVEPAPEGSVLVLVLKDLNFQGKTINLG → MSPALPGQVRVEPAAPCPAPDEHSGWGTDAPAG

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Phosphatidylinositol 4-phosphate 5-kinase-like protein 1		PRO_0000285757
Regions
	Domain	38 – 395	358	PIPK
Natural variations
	Alternative sequence	1 – 224	224	Missing in isoform 2.		VSP_024902
	Alternative sequence	225 – 257	33	CEVSR…TINLG → MSPALPGQVRVEPAAPCPAP DEHSGWGTDAPAG in isoform 2.		VSP_024903

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 218E24E583B1EF78	FASTA	396	44,778
	10 20 30 40 50 60 MAAPSPGPRE ILAPSPEAGR RAAASSSGHR GLLWRLRDKQ CRLGLFEIGP GHELHQVMCL 70 80 90 100 110 120 MQAGLWAATQ VSMDHPPTGL PTEEDFSEVL TQVHEGFELG TLAGPVFARL RRSLGLAEED 130 140 150 160 170 180 YQAALGPSRP YLQFLSTSKS KASFFLSHDQ RFFLKTLRSR EVQALLAHLP RYVHHLQRHP 190 200 210 220 230 240 HSLLARVLGV HSLRVARGKK KYFIVMQSVF YPAGRISERY DIKGCEVSRW VEPAPEGSVL 250 260 270 280 290 300 VLVLKDLNFQ GKTINLGPQR SWFLRQMELD TAFLRELNVL DYSLLMAFQR LHEDERGPGS 310 320 330 340 350 360 SLIFRTARSI RGAQSAEESG AQNRRLLPDA PNALHIVDGP EHRYFLGLVD LTTVYGLRKR 370 380 390 LEQLWKTLRY PGRTFSTVSP ACYARRLCQW VEAHTE
	Isoform 2 [UniParc]. Checksum: 7CF20070FF2113DB	FASTA	172	19,385
	10 20 30 40 50 60 MSPALPGQVR VEPAAPCPAP DEHSGWGTDA PAGPQRSWFL RQMELDTAFL RELNVLDYSL 70 80 90 100 110 120 LMAFQRLHED ERGPGSSLIF RTARSIRGAQ SAEESGAQNR RLLPDAPNAL HIVDGPEHRY 130 140 150 160 170 FLGLVDLTTV YGLRKRLEQL WKTLRYPGRT FSTVSPACYA RRLCQWVEAH TE

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Hereford. Tissue: Thalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT021629 mRNA. Translation: AAX46476.1. BC118210 mRNA. Translation: AAI18211.1.
IPI	IPI00718004. IPI00782887.
RefSeq	NP_001069315.2. NM_001075847.2.
UniGene	Bt.37390.
3D structure databases
ProteinModelPortal	Q17QS4.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000004392.
Proteomic databases
PRIDE	Q17QS4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	523736.
KEGG	bta:523736.
Organism-specific databases
CTD	138429.
Phylogenomic databases
eggNOG	COG5253.
HOGENOM	HOG000115541.
HOVERGEN	HBG097351.
KO	K13712.
Family and domain databases
Gene3D	3.30.800.10. 1 hit. 3.30.810.10. 1 hit.
InterPro	IPR023610. PInositol-4-P-5-kinase. IPR027483. PInositol-4-P-5-kinase_C. IPR002498. PInositol-4-P-5-kinase_core. IPR027484. PInositol-4-P-5-kinase_N. [Graphical view]
PANTHER	PTHR23086. PTHR23086. 1 hit.
Pfam	PF01504. PIP5K. 1 hit. [Graphical view]
PROSITE	PS51455. PIPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20873812.

Entry information

Entry name

PI5L1_BOVIN

Accession

Primary (citable) accession number: Q17QS4
Secondary accession number(s): Q58DG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 25, 2006
Last modified:	May 29, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program