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Protein

Thiopurine S-methyltransferase

Gene

TPMT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401SubstrateBy similarity
Binding sitei69 – 691S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei90 – 901S-adenosyl-L-methionineBy similarity
Binding sitei152 – 1521S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-BTA-156581. Methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiopurine S-methyltransferase (EC:2.1.1.67)
Alternative name(s):
Thiopurine methyltransferase
Gene namesi
Name:TPMT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Thiopurine S-methyltransferasePRO_0000278660Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ17QQ2.
PeptideAtlasiQ17QQ2.
PRIDEiQ17QQ2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019300.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025698.

Structurei

3D structure databases

ProteinModelPortaliQ17QQ2.
SMRiQ17QQ2. Positions 17-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 4012S-adenosyl-L-methionine bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IGTR. Eukaryota.
ENOG4111GNF. LUCA.
GeneTreeiENSGT00390000016823.
HOGENOMiHOG000276919.
HOVERGENiHBG003037.
InParanoidiQ17QQ2.
KOiK00569.
OMAiKWVNGKT.
OrthoDBiEOG091G0VS2.
TreeFamiTF328951.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00812. Thiopur_methtran. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025835. Thiopurine_S-MeTrfase.
IPR008854. TPMT.
[Graphical view]
PfamiPF05724. TPMT. 1 hit.
[Graphical view]
PIRSFiPIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51585. SAM_MT_TPMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17QQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSRALLDS EEYPNTEAQK DRVLTLEEWQ EKWVNHKTGF HQEQGHQLLK
60 70 80 90 100
KYLDTFLKGE KALRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIRDFF
110 120 130 140 150
TEQNLSYSEE PIMEIPGAKI FKSSSGNISL YCCNLFDLPR ANIGKFDRIW
160 170 180 190 200
DRGALVAVNP SDRKRYSDVM LSLTRPGFRY LLSVFSYDPT KHAGPPFYVT
210 220 230 240
DGEVKKLFGS VCNIQCLEKV DVFEERHKSW GIDQIIERLY LFTEK
Length:245
Mass (Da):28,335
Last modified:July 25, 2006 - v1
Checksum:iC734333BEEC2828F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC118238 mRNA. Translation: AAI18239.1.
RefSeqiNP_001068999.1. NM_001075531.1.
XP_005223808.1. XM_005223751.3.
XP_005223809.1. XM_005223752.3.
XP_005223811.1. XM_005223754.3.
UniGeneiBt.15903.

Genome annotation databases

EnsembliENSBTAT00000025698; ENSBTAP00000025698; ENSBTAG00000019300.
GeneIDi511644.
KEGGibta:511644.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC118238 mRNA. Translation: AAI18239.1.
RefSeqiNP_001068999.1. NM_001075531.1.
XP_005223808.1. XM_005223751.3.
XP_005223809.1. XM_005223752.3.
XP_005223811.1. XM_005223754.3.
UniGeneiBt.15903.

3D structure databases

ProteinModelPortaliQ17QQ2.
SMRiQ17QQ2. Positions 17-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025698.

Proteomic databases

PaxDbiQ17QQ2.
PeptideAtlasiQ17QQ2.
PRIDEiQ17QQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000025698; ENSBTAP00000025698; ENSBTAG00000019300.
GeneIDi511644.
KEGGibta:511644.

Organism-specific databases

CTDi7172.

Phylogenomic databases

eggNOGiENOG410IGTR. Eukaryota.
ENOG4111GNF. LUCA.
GeneTreeiENSGT00390000016823.
HOGENOMiHOG000276919.
HOVERGENiHBG003037.
InParanoidiQ17QQ2.
KOiK00569.
OMAiKWVNGKT.
OrthoDBiEOG091G0VS2.
TreeFamiTF328951.

Enzyme and pathway databases

ReactomeiR-BTA-156581. Methylation.

Gene expression databases

BgeeiENSBTAG00000019300.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00812. Thiopur_methtran. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025835. Thiopurine_S-MeTrfase.
IPR008854. TPMT.
[Graphical view]
PfamiPF05724. TPMT. 1 hit.
[Graphical view]
PIRSFiPIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51585. SAM_MT_TPMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPMT_BOVIN
AccessioniPrimary (citable) accession number: Q17QQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.