Reviewed, UniProtKB/Swiss-Prot Q17QQ2 (TPMT_BOVIN)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:

TPMT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine By similarity.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Thiopurine S-methyltransferase		PRO_0000278660
Sites
	Binding site	33	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	152	1	S-adenosyl-L-methionine By similarity
Amino acid modifications
	Modified residue	58	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q17QQ2-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: C734333BEEC2828F	FASTA	245	28,335
	10 20 30 40 50 60 MGDSRALLDS EEYPNTEAQK DRVLTLEEWQ EKWVNHKTGF HQEQGHQLLK KYLDTFLKGE 70 80 90 100 110 120 KALRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIRDFF TEQNLSYSEE PIMEIPGAKI 130 140 150 160 170 180 FKSSSGNISL YCCNLFDLPR ANIGKFDRIW DRGALVAVNP SDRKRYSDVM LSLTRPGFRY 190 200 210 220 230 240 LLSVFSYDPT KHAGPPFYVT DGEVKKLFGS VCNIQCLEKV DVFEERHKSW GIDQIIERLY LFTEK

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC118238 mRNA. Translation: AAI18239.1.
IPI	IPI00705007.
RefSeq	NP_001068999.1.
UniGene	Bt.15903
3D structure databases
SMR	Q17QQ2. Positions 17-245.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000025698; ENSBTAP00000025698; ENSBTAG00000019300; Bos taurus. [Genome view]
GeneID	511644.
KEGG	bta:511644.
Organism-specific databases
CTD	511644.
Phylogenomic databases
HOVERGEN	Q17QQ2.
OMA	PPFAVSP.
Enzyme and pathway databases
BRENDA	2.1.1.67. 251.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_BOVIN

Accession

Primary (citable) accession number: Q17QQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 25, 2006
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)