Q17QK9 (NAA60_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-alpha-acetyltransferase 60
EC=2.3.1.48
EC=2.3.1.88

Alternative name(s):
N-acetyltransferase 15
NatF catalytic subunit

Gene names

Name:	NAA60
Synonyms:	NAT15

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase By similarity.
Catalytic activity	Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA. Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subcellular location	Golgi apparatus membrane By similarity.
Post-translational modification	Acetylated: autoacetylation is required for optimal acetyltransferase activity By similarity.
Sequence similarities	Belongs to the acetyltransferase family. NAA60 subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Chromosome partition
Cellular component	Golgi apparatus Membrane
Molecular function	Acyltransferase Chromatin regulator Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-terminal peptidyl-methionine acetylation Inferred from sequence or structural similarity. Source: UniProtKB cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB chromosome segregation Inferred from sequence or structural similarity. Source: UniProtKB nucleosome assembly Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	H4 histone acetyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB peptide alpha-N-acetyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	N-alpha-acetyltransferase 60		PRO_0000321565
Regions
	Domain	13 – 182	170	N-acetyltransferase
Amino acid modifications
	Modified residue	79	1	N6-acetyllysine; by autocatalysis By similarity
	Modified residue	105	1	N6-acetyllysine; by autocatalysis By similarity
	Modified residue	109	1	N6-acetyllysine; by autocatalysis By similarity
	Modified residue	121	1	N6-acetyllysine; by autocatalysis By similarity
	Modified residue	156	1	N6-acetyllysine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q17QK9 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: E2E25F6BA8B13683	FASTA	242	27,459
	10 20 30 40 50 60 MTEAVPSSAL SEVSLRLLCH DDIDTVKHLC GDWFPIEYPD SWYRDITSNK KFFSLAATYR 70 80 90 100 110 120 GDIVGMIVAE IKNRTKIHKE DGDILASSFS VDTQVAYILS LGVVKEFRKH GIGSLLLESL 130 140 150 160 170 180 KDHISTTAQD HCKAIYLHVL TTNNTAISFY ENRDFKQHHY LPYYYSIRGV LKDGFTYVLY 190 200 210 220 230 240 INGGHPPWTI LDYIQHLGSA LANLSPCSIP HRIYRQAQSL LCSFLPWSSI STKGGIEYSR TM

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC118298 mRNA. Translation: AAI18299.1.
IPI	IPI00718706.
RefSeq	NP_001069117.1. NM_001075649.1.
UniGene	Bt.9071.
3D structure databases
ProteinModelPortal	Q17QK9.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000006410.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000006410; ENSBTAP00000006410; ENSBTAG00000004875.
GeneID	514154.
KEGG	bta:514154.
Organism-specific databases
CTD	79903.
Phylogenomic databases
eggNOG	NOG276305.
GeneTree	ENSGT00390000008314.
HOGENOM	HOG000280717.
HOVERGEN	HBG061680.
InParanoid	Q17QK9.
OMA	PWTILYP.
OrthoDB	EOG45TCNW.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20871196.

Entry information

Entry name

NAA60_BOVIN

Accession

Primary (citable) accession number: Q17QK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 25, 2006
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program