Reviewed, UniProtKB/Swiss-Prot Q17QK8 (SPRE_BOVIN)

Names and origin

Protein names

Recommended name:
    Sepiapterin reductase
      Short name=SPR
    EC=1.1.1.153

Gene names

Name:

SPR

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin By similarity.
Catalytic activity	7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH. Tetrahydrobiopterin + 2 NADP+ = 6-pyruvoyl-5,6,7,8-tetrahydropterin + 2 NADPH.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the sepiapterin reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro sepiapterin reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Sepiapterin reductase		PRO_0000327641
Regions
	Nucleotide binding	19 – 45	27	NADP By similarity
	Region	34 – 38	5	Pterin binding By similarity
Experimental info
	Sequence conflict	236	1	E → Q in ABI79460. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q17QK8-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 4F2B7089A40F8C52	FASTA	267	28,939
	10 20 30 40 50 60 MEGSVGKVGG LGRTLCVLTG ASRGFGRTLA QVLAPLMSPR SVLVLSARND EALRQLETEL 70 80 90 100 110 120 GAEWPGLRIV RVPADLGAET GLQQLVGALC DLPRPEGLQR VLLINNAGTL GDVSKRWVDL 130 140 150 160 170 180 TDPTEVNNYW TLNLTSTLCL TSSILQAFPD SPGLSRTVVN ISSICALQPF KGWGLYCAGK 190 200 210 220 230 240 AARNMMFQVL AAEEPSVRVL SYGPGPLDTD MQQLARETSV DPDLRKSLQE LKRKGELVDC 250 260 KISAQKLLSL LQNDKFESGA HIDFYDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of cDNA encoding bovine sepiapterin reductase isoform 2." Pung Y.F., Cai H. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ978331 mRNA. Translation: ABI79460.1. BC118299 mRNA. Translation: AAI18300.1.
IPI	IPI00709782.
RefSeq	NP_001069471.1.
UniGene	Bt.3075
3D structure databases
SMR	Q17QK8. Positions 11-267.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000021961. Bos taurus. [Contig view]
GeneID	533836.
KEGG	bta:533836.
Phylogenomic databases
HOVERGEN	Q17QK8.
Enzyme and pathway databases
BRENDA	1.1.1.153. 251.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR006393. Sepiapterin_red. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01500. sepiapter_red. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SPRE_BOVIN

Accession

Primary (citable) accession number: Q17QK8
Secondary accession number(s): A7YKV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 25, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)