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Protein

AP-2 complex subunit sigma

Gene

AP2S1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-BTA-171052. LDL-mediated lipid transport.
R-BTA-177504. Retrograde neurotrophin signalling.
R-BTA-2132295. MHC class II antigen presentation.
R-BTA-416993. Trafficking of GluR2-containing AMPA receptors.
R-BTA-437239. Recycling pathway of L1.
R-BTA-5099900. WNT5A-dependent internalization of FZD4.
R-BTA-5140745. WNT5A-dependent internalization of FZD2, FZD5 and ROR2.
R-BTA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-BTA-8856828. Clathrin-mediated endocytosis.
R-BTA-8866427. VLDLR internalisation and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit sigma
Alternative name(s):
Adaptor protein complex AP-2 subunit sigma
Adaptor-related protein complex 2 subunit sigma
Clathrin assembly protein 2 sigma small chain
Clathrin coat assembly protein AP17
Clathrin coat-associated protein AP17
Plasma membrane adaptor AP-2 17 kDa protein
Sigma2-adaptin
Gene namesi
Name:AP2S1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002838051 – 142AP-2 complex subunit sigmaAdd BLAST142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei140PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ17QC5.
PRIDEiQ17QC5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000010584.

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013989.

Structurei

3D structure databases

ProteinModelPortaliQ17QC5.
SMRiQ17QC5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0935. Eukaryota.
COG5030. LUCA.
GeneTreeiENSGT00530000062839.
HOGENOMiHOG000185227.
HOVERGENiHBG050517.
InParanoidiQ17QC5.
KOiK11827.
OMAiSFFDNVC.
OrthoDBiEOG091G0QZV.
TreeFamiTF300139.

Family and domain databases

InterProiIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERiPTHR11753. PTHR11753. 1 hit.
PfamiPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17QC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRFILIQNR AGKTRLAKWY MQFDDDEKQK LIEEVHAVVT VRDAKHTNFV
60 70 80 90 100
EFRNFKIIYR RYAGLYFCIC VDVNDNNLAY LEAIHNFVEV LNEYFHNVCE
110 120 130 140
LDLVFNFYKV YTVVDEMFLA GEIRETSQTK VLKQLLMLQS LE
Length:142
Mass (Da):17,018
Last modified:July 25, 2006 - v1
Checksum:iCA3FD868C65AEDF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC118437 mRNA. Translation: AAI18438.1.
RefSeqiNP_001069546.1. NM_001076078.2.
UniGeneiBt.6673.

Genome annotation databases

EnsembliENSBTAT00000013989; ENSBTAP00000013989; ENSBTAG00000010584.
GeneIDi536553.
KEGGibta:536553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC118437 mRNA. Translation: AAI18438.1.
RefSeqiNP_001069546.1. NM_001076078.2.
UniGeneiBt.6673.

3D structure databases

ProteinModelPortaliQ17QC5.
SMRiQ17QC5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013989.

Proteomic databases

PaxDbiQ17QC5.
PRIDEiQ17QC5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013989; ENSBTAP00000013989; ENSBTAG00000010584.
GeneIDi536553.
KEGGibta:536553.

Organism-specific databases

CTDi1175.

Phylogenomic databases

eggNOGiKOG0935. Eukaryota.
COG5030. LUCA.
GeneTreeiENSGT00530000062839.
HOGENOMiHOG000185227.
HOVERGENiHBG050517.
InParanoidiQ17QC5.
KOiK11827.
OMAiSFFDNVC.
OrthoDBiEOG091G0QZV.
TreeFamiTF300139.

Enzyme and pathway databases

ReactomeiR-BTA-171052. LDL-mediated lipid transport.
R-BTA-177504. Retrograde neurotrophin signalling.
R-BTA-2132295. MHC class II antigen presentation.
R-BTA-416993. Trafficking of GluR2-containing AMPA receptors.
R-BTA-437239. Recycling pathway of L1.
R-BTA-5099900. WNT5A-dependent internalization of FZD4.
R-BTA-5140745. WNT5A-dependent internalization of FZD2, FZD5 and ROR2.
R-BTA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-BTA-8856828. Clathrin-mediated endocytosis.
R-BTA-8866427. VLDLR internalisation and degradation.

Gene expression databases

BgeeiENSBTAG00000010584.

Family and domain databases

InterProiIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERiPTHR11753. PTHR11753. 1 hit.
PfamiPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP2S1_BOVIN
AccessioniPrimary (citable) accession number: Q17QC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 25, 2006
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.