Reviewed, UniProtKB/Swiss-Prot Q17M80 (MMSA_AEDAE)

Names and origin

Protein names

Recommended name:
    Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
      Short name=Malonate-semialdehyde dehydrogenase [acylating]
      Short name=MMSDH
    EC=1.2.1.27
    EC=1.2.1.18

Gene names

ORF Names:

AAEL001134

Organism

Aedes aegypti (Yellowfever mosquito) (Culex aegypti) [Complete proteome]

Taxonomic identifier

7159 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Culicini › Aedes › Stegomyia

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA By similarity. UniProtKB Q02252
Catalytic activity	2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. UniProtKB Q02252 3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. UniProtKB Q02252
Subunit structure	Homotetramer By similarity. UniProtKB Q02252
Subcellular location	Mitochondrion By similarity UniProtKB Q02252.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW thymine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB valine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from sequence or structural similarity. Source: UniProtKB methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 521		Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial		PRO_0000312823
Regions
	Nucleotide binding	196 – 200	5	NAD Potential
	Nucleotide binding	248 – 253	6	NAD Potential
Sites
	Active site	304	1	Nucleophile By similarity UniProtKB Q02252
	Binding site	404	1	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q17M80-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 9FC4A9E85D5F39A7	FASTA	521	56,572
	10 20 30 40 50 60 MALVRLLGVE CRNALQRSYS TASVPTTKMF IDGKFVDSKT TEWIDLHDPA TNKVVTRVPK 70 80 90 100 110 120 CTQDEMESAV ESSKKAFKTW SQTSILGRQQ VMFKLQHLIR NNMSELAKNI TKEQGKTLVD 130 140 150 160 170 180 AEGDVLRGLQ VVEHCCSITS LQMGETVPNI AKDMDTYSYT LPLGVTAGIC PFNFPAMIPL 190 200 210 220 230 240 WMFPVAITCG NTSIIKPSER VPGATMMLME LLNEAGCPPG VVNVIHGAHD AVNFICDNPT 250 260 270 280 290 300 IKAVSFVGSD QAGKYIYERA GRNGKRVQSN MGAKNHGVIM ADANKENTLN QLAGAAFGAA 310 320 330 340 350 360 GQRCMALSTA VFVGEAKQWI PDLVERARKL KVNAGHVPGT DVGPVISPQS KQRINELVES 370 380 390 400 410 420 GVKEGAKLVL DGRSIKVENF ENGNFVGPTI LTDVSTNMKC YTEEIFGPVL VCLTVDTVDE 430 440 450 460 470 480 AVEMINNNPY GNGTAIFTTN GATARKFVNE IDVGQVGVNV PIPVPLPMFS FTGSRGSFMG 490 500 510 520 DCHFYGKQGV KFYTQTKTVT QLWREGDVSH TKAAVAMPTM K

References

[1]

"Genome sequence of Aedes aegypti, a major arbovirus vector." Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W. Science 316:1718-1723(2007) [PubMed: 17510324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Liverpool.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH477208 Genomic DNA. Translation: EAT47764.1.
RefSeq	XP_001652386.1.
UniGene	Aae.2920
3D structure databases
SMR	Q17M80. Positions 24-504.
ModBase	Search...
Protein-protein interaction databases
STRING	Q17M80.
Genome annotation databases
GeneID	5568558.
KEGG	aag:AaeL_AAEL001134.
VectorBase	AAEL001134. Aedes aegypti.
Phylogenomic databases
eggNOG	inNOG06001.
OMA	VEHAANV.
OrthoDB	EOG95QHM2.
PhylomeDB	Q17M80.
Enzyme and pathway databases
BRENDA	1.2.1.18. 1026. 1.2.1.27. 1026.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMSA_AEDAE

Accession

Primary (citable) accession number: Q17M80

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	July 25, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)