Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q17IE8 (CDK8_AEDAE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 8

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit Cdk8
Mediator of RNA polymerase II transcription subunit Cdk8
Gene names
Name:Cdk8
ORF Names:AAEL002395
OrganismAedes aegypti (Yellowfever mosquito) (Culex aegypti) [Reference proteome]
Taxonomic identifier7159 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Component of the Cdk8 module of the Mediator complex By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence EAT46429.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Cyclin-dependent kinase 8
PRO_0000312929

Regions

Domain21 – 335315Protein kinase
Nucleotide binding27 – 359ATP By similarity
Compositional bias373 – 499127Gln-rich

Sites

Active site1511Proton acceptor By similarity
Binding site521ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q17IE8 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: 1D177BCD71970FFB

FASTA50158,257
        10         20         30         40         50         60 
MDYEFKMKTQ QERAKVEDLF EYEGCKVGRG TYGHVYKAHR KESNDTKDYA LKQIEGTGLS 

        70         80         90        100        110        120 
MSACREIALL RELKHPNVIN LIRVFLSHTD RKVWLLFDYA EHDLWHIIKF HRAAKATKKP 

       130        140        150        160        170        180 
VMVPKGMVKS LLYQILDGIH YLHSNWVLHR DLKPANILVM GEGNERGRVK IADMGFARLF 

       190        200        210        220        230        240 
NAPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED 

       250        260        270        280        290        300 
IKTSNPYHHD QLDRIFNVMG FPQDKDWEDI RKMPEHHTLT KDFKRSNYAN CSLVKYMERH 

       310        320        330        340        350        360 
KIKPDSKAFH LLQKLLLMDP NKRITSEQAM QDPYFSEDPM PTADVFAGCP IPYPKREFLT 

       370        380        390        400        410        420 
DEDQDDKGES KRQQQQQQQQ QANQQAQQNQ QQQANHQNQQ QNQQQQANQQ NQQSQQQQQQ 

       430        440        450        460        470        480 
QQMQQQQQAQ AGGMDHSAAK RVRMSGPNGQ PGNGMNQQDY QVQQAQQAAA AAAAAQQQQQ 

       490        500 
QQQQQQMMFN NQQQGGFQQR Y 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH477240 Genomic DNA. Translation: EAT46429.1. Different initiation.
RefSeqXP_001655140.1. XM_001655090.1.
UniGeneAae.7756.

3D structure databases

ProteinModelPortalQ17IE8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7159.AAEL002395-PA.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5574480.
KEGGaag:AaeL_AAEL002395.
VectorBaseAAEL002395. Aedes aegypti.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK02208.
OrthoDBEOG76739K.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK8_AEDAE
AccessionPrimary (citable) accession number: Q17IE8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: February 19, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families