Q17IE8 (CDK8_AEDAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 8
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit Cdk8
Mediator of RNA polymerase II transcription subunit Cdk8

Gene names

Name:	Cdk8
ORF Names:	AAEL002395

Organism

Aedes aegypti (Yellowfever mosquito) (Culex aegypti) [Reference proteome]

Taxonomic identifier

7159 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Culicoidea › Culicidae › Culicinae › Aedini › Aedes › Stegomyia

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the Cdk8 module of the Mediator complex By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence EAT46429.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB protein phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Cyclin-dependent kinase 8		PRO_0000312929
Regions
	Domain	21 – 335	315	Protein kinase
	Nucleotide binding	27 – 35	9	ATP By similarity
	Compositional bias	373 – 499	127	Gln-rich
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q17IE8 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 1D177BCD71970FFB	FASTA	501	58,257
	10 20 30 40 50 60 MDYEFKMKTQ QERAKVEDLF EYEGCKVGRG TYGHVYKAHR KESNDTKDYA LKQIEGTGLS 70 80 90 100 110 120 MSACREIALL RELKHPNVIN LIRVFLSHTD RKVWLLFDYA EHDLWHIIKF HRAAKATKKP 130 140 150 160 170 180 VMVPKGMVKS LLYQILDGIH YLHSNWVLHR DLKPANILVM GEGNERGRVK IADMGFARLF 190 200 210 220 230 240 NAPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED 250 260 270 280 290 300 IKTSNPYHHD QLDRIFNVMG FPQDKDWEDI RKMPEHHTLT KDFKRSNYAN CSLVKYMERH 310 320 330 340 350 360 KIKPDSKAFH LLQKLLLMDP NKRITSEQAM QDPYFSEDPM PTADVFAGCP IPYPKREFLT 370 380 390 400 410 420 DEDQDDKGES KRQQQQQQQQ QANQQAQQNQ QQQANHQNQQ QNQQQQANQQ NQQSQQQQQQ 430 440 450 460 470 480 QQMQQQQQAQ AGGMDHSAAK RVRMSGPNGQ PGNGMNQQDY QVQQAQQAAA AAAAAQQQQQ 490 500 QQQQQQMMFN NQQQGGFQQR Y

References

[1]

"Genome sequence of Aedes aegypti, a major arbovirus vector." Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W. Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LVPib12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH477240 Genomic DNA. Translation: EAT46429.1. Different initiation.
RefSeq	XP_001655140.1. XM_001655090.1.
UniGene	Aae.7756.
3D structure databases
ProteinModelPortal	Q17IE8.
ModBase	Search...
Protein-protein interaction databases
STRING	7159.AAEL002395-PA.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5574480.
KEGG	aag:AaeL_AAEL002395.
VectorBase	AAEL002395. Aedes aegypti.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K02208.
OrthoDB	EOG4ZS7JJ.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK8_AEDAE

Accession

Primary (citable) accession number: Q17IE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)