Reviewed, UniProtKB/Swiss-Prot Q17EN4 (HOT_AEDAE)

Names and origin

Protein names

Recommended name:
    Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
      Short name=HOT
    EC=1.1.99.24

Gene names

ORF Names:

AAEL003729

Organism

Aedes aegypti (Yellowfever mosquito)

Taxonomic identifier

7159 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Culicini › Aedes › Stegomyia

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
Subcellular location	MitochondrionBy similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 462		Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial		PRO_0000323003

Sequences

	Sequence		Length	Mass (Da)
	Q17EN4-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 294DCCE1FDF80BA1	FASTA	462	50,140
	10 20 30 40 50 60 MMSKARISSL MKVISSGSCS CPAHSRSSTG GLNVSSENEY AFEMSSSTIR YGPGVSKELG 70 80 90 100 110 120 HDLQNLNAKN VCIVTDRNVA KLNSVKVAFD SLTRCGIQYQ VYDETRVEPT DQSLLHAAEY 130 140 150 160 170 180 ARQNKFDSFV AIGGGSVIDT CKVANLFSAD REAEFLDYVN APIGKAKEVN VKLKPLIAVP 190 200 210 220 230 240 TTAGTGSETT GVVIFDYKPL HAKTGISSKY LRPQLGLIDP LHTLSQPEKV AAYCGFDVFC 250 260 270 280 290 300 HALESFTAIP YTERGSAPMN PNLRPPYQGS NPISDVWARF ALKIIRENFI SAVFNQDDLK 310 320 330 340 350 360 ARSNMHLAST MAGVGFGNAG VHLCHGLSYP ISGHVKKFVP DGYSGDHPII PHGLSVVMTA 370 380 390 400 410 420 PAVFKFTAAS CPERHLEAAE LLGANVSKAN RNDAGSILSD VVRQYMYKLK IENGLDSLGF 430 440 450 460 TRDDIQSLVK GTLPQERITK LAPRAQTEED LASLFENSMT VY

References

[1]

"Genome sequence of Aedes aegypti, a major arbovirus vector." Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W. Science 316:1718-1723(2007) [PubMed: 17510324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Liverpool.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH477280 Genomic DNA. Translation: EAT44958.1.
RefSeq	XP_001664064.1.
UniGene	Aae.6969
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5578877.
KEGG	aag:AaeL_AAEL003729.
VectorBase	AAEL003729. Aedes aegypti.
Family and domain databases
InterPro	IPR001670. Fe_AlcDHase. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOT_AEDAE

Accession

Primary (citable) accession number: Q17EN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 25, 2006
Last modified:	November 25, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)