Q17967 (PDI1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide-isomerase 1
Short name=PDI 1
EC=5.3.4.1

Alternative name(s):
Prolyl 4-hydroxylase subunit beta-1

Gene names

Name:	pdi-1
ORF Names:	C14B1.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from direct assay Ref.1. Source: WormBase protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro protein-glutamine gamma-glutamyltransferase activity Inferred from direct assay. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 485	465	Protein disulfide-isomerase 1		PRO_0000034203
Regions
	Domain	21 – 130	110	Thioredoxin 1
	Domain	342 – 470	129	Thioredoxin 2
	Motif	482 – 485	4	Prevents secretion from ER Potential
Sites
	Active site	393	1	Nucleophile By similarity
	Active site	396	1	Nucleophile By similarity
	Site	394	1	Contributes to redox potential value By similarity
	Site	395	1	Contributes to redox potential value By similarity
	Site	456	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Disulfide bond	52 ↔ 55		Redox-active By similarity
	Disulfide bond	393 ↔ 396		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q17967 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 74070D655E6A415C	FASTA	485	53,436
	10 20 30 40 50 60 MSLSVSFIFL LVASIGAVVA DSENVLVLTE SNFEETINGN EFVLVKFYAP WCVHCKSLAP 70 80 90 100 110 120 KYDEAADLLK EEGSDIKLAK VDATENQALA SKFEVRGYPT ILYFKSGKPT KYTGGRATAQ 130 140 150 160 170 180 IVDWVKKKSG PTVTTVESVE QLEELKGKTR VVVLGYFKDA KSDAATIYNE VADSVDDAFF 190 200 210 220 230 240 AVAGSAEVAA AASLNEDGVA LIRTDGDDSE TSTIAEAEIT NTIALKQWLH AYKLSAVTEF 250 260 270 280 290 300 THESAQEIVG GDLKKFHFLI IRKSDSSFDE TIAKFTEVAK KFRAKIVFVL LDVDVEENAR 310 320 330 340 350 360 ILEFLGVDAK NTPANRIVSL ADQVEKFKPQ EGEDFEAFTN SYLEGKSAQD LKAQDLPEDW 370 380 390 400 410 420 NALPVKVLVA SNFNEIALDE TKTVFVKFYA PWCGHCKQLV PVWDELAEKY ESNPNVVIAK 430 440 450 460 470 480 LDATLNELAD VKVNSFPTLK LWPAGSSTPV DYDGDRNLEK FEEFVNKYAG SASESETASQ DHEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Baculovirus expression of two protein disulphide isomerase isoforms from Caenorhabditis elegans and characterization of prolyl 4-hydroxylases containing one of these polypeptides as their beta subunit." Veijola J., Annunen P., Koivunen P., Page A.P., Pihlajaniemi T., Kivirikko K.I. Biochem. J. 317:721-729(1996) [PubMed: 8760355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U95074 Genomic DNA. Translation: AAB94647.1. Z37139 Genomic DNA. Translation: CAA85491.1.
PIR	S71863.
RefSeq	NP_497746.1. NM_065345.4.
UniGene	Cel.23340.
3D structure databases
ProteinModelPortal	Q17967.
SMR	Q17967. Positions 20-476.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24653N.
IntAct	Q17967. 1 interaction.
MINT	MINT-226883.
STRING	Q17967.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C14B1.1.1; C14B1.1.1; C14B1.1. C14B1.1.2; C14B1.1.2; C14B1.1.
GeneID	175472.
KEGG	cel:C14B1.1.
UCSC	C14B1.1.1. c. elegans.
Organism-specific databases
CTD	175472.
WormBase	C14B1.1; CE00897; WBGene00003962; pdi-1.
Phylogenomic databases
eggNOG	meNOG04114.
GeneTree	EMGT00050000002774.
HOGENOM	HBG627841.
InParanoid	Q17967.
OMA	PANRIVS.
PhylomeDB	Q17967.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR005792. Prot_disulphide_isomerase. IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 3 hits.
KO	K09580.
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. Pdi_dom. 2 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	888312.

Entry information

Entry name

PDI1_CAEEL

Accession

Primary (citable) accession number: Q17967

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project