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Protein

WD repeat-containing protein wdr-5.1

Gene

wdr-5.1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to histone modification (PubMed:16710447, PubMed:17967446, PubMed:20188723, PubMed:20555324, PubMed:21455483, PubMed:22012258). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed:21455483). Required for di- and trimethylation, particularly for the trimethylation at 'Lys-4' of histone H3 (PubMed:20555324, PubMed:21455483, PubMed:24682813). Not required for demethylation of histone H3 'Lys-27' (PubMed:21455483). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, germline establishment, maintenance and function (PubMed:21455483). Required for RNA interference with probable antagonistic role against hpl-2 function (PubMed:17967446). Regulates longevity by limiting lifespan (PubMed:17967446, PubMed:20555324, PubMed:22012258). Plays a role in vulval cell fate specification by acting in the synthetic multivulva pathway independent of set-2 (PubMed:17967446). Sex determining protein required in the germline to promote the spermatogenesis to oogenesis switch during the late larval stages of development (PubMed:24682813). Acts with the sex determining factor tra-1, and redundantly with wdr-5.2, to regulate fog-3 expression, which in turn determines germ cell fate (PubMed:24682813).7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei149Important for interaction with histone H3By similarity1
Sitei175Important for interaction with histone H3By similarity1
Sitei305Important for interaction with histone H3By similarity1
Sitei364Important for interaction with histone H3By similarity1

GO - Molecular functioni

  • RNA polymerase II transcription factor binding Source: WormBase

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • gene silencing by RNA Source: UniProtKB-KW
  • histone H3-K4 methylation Source: WormBase
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • transdifferentiation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Developmental protein

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation

Enzyme and pathway databases

SignaLinkiQ17963.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein wdr-5.1
Gene namesi
Name:wdr-5.1
Synonyms:swd-3.1, tag-125
ORF Names:C14B1.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC14B1.4; CE00901; WBGene00006474; wdr-5.1.

Subcellular locationi

  • Nucleus 2 Publications

  • Note: Localized on chromatin in the nucleus.1 Publication

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Increase in life span (PubMed:17967446). Longer time span to reach adulthood and reduced brood size resulting in sterility between generations F3 and F4 (PubMed:17967446). Associated embryonic lethality and additional somatic defects at elevated temperatures (PubMed:17967446). Defects in germ cells including defective sperm development and endomitotic oocytes (PubMed:17967446, PubMed:24682813). Significantly reduced H3 'Lys-4' trimethylation in both embryonic and adult germ cells in a sex-independent manner (PubMed:17967446). Increased fog-3 expression at 20 degress Celsius (PubMed:24682813). At 20 and 25 degrees Celsius, double knockdown mutants with wdr-5.2 have increased fog-3 expression (PubMed:24682813). At 25 degrees Celsius, these mutants have increased wdr-5.2 and fog-1 expression, reduced brood size accompanied by 42% embryonic lethality with 100% of the surviving progeny being sterile (PubMed:24682813). Surviving progeny display enhanced defects in the spermatogenesis to oogenesis transition compared to the single wdr-5.1 knockout with 88% of the gonads only containing sperm (PubMed:24682813). The remaining germ cells in the gonads switch to oogenesis, but the oocytes display either an endoreplication or endomitotic phenotype (PubMed:24682813). Germ cells also have increased expression of the sex determining factor tra-1 in the cytoplasm and as a result there is reduced binding of tra-1 to the fog-3 promoter (PubMed:24682813).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000515031 – 376WD repeat-containing protein wdr-5.1Add BLAST376

Proteomic databases

EPDiQ17963.
PaxDbiQ17963.
PeptideAtlasiQ17963.
PRIDEiQ17963.

Expressioni

Tissue specificityi

Enriched in the germline (PubMed:17967446, PubMed:24682813). Detected in all nuclei of the embryo (PubMed:17967446). In larvae, expression is detected in the nuclei of seam cells, somatic gonad precursor cells Z1 and Z4, vulval precursor cells, distal tip cells, hypodermal cells, intestinal and muscle cells (PubMed:17967446). Also detected in the neurons from the ventral nerve cord, head and tail region (PubMed:17967446). Expressed in the head and tail region, intestinal cells, muscle cells, cells of the vulva, spermatheca and sheath cells in adults (PubMed:17967446).2 Publications

Developmental stagei

Detected from the 20 cell embryo stage and continues through to adult, although in a restricted manner.1 Publication

Gene expression databases

BgeeiWBGene00006474.

Interactioni

Subunit structurei

Interacts with histone H3 both unmethylated and methylated at 'Lys-4'.1 Publication

GO - Molecular functioni

  • RNA polymerase II transcription factor binding Source: WormBase

Protein-protein interaction databases

DIPiDIP-55118N.
IntActiQ17963. 1 interactor.
STRINGi6239.C14B1.4.

Structurei

3D structure databases

ProteinModelPortaliQ17963.
SMRiQ17963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati85 – 115WD 1Add BLAST31
Repeati127 – 157WD 2Add BLAST31
Repeati169 – 199WD 3Add BLAST31
Repeati211 – 241WD 4Add BLAST31
Repeati254 – 284WD 5Add BLAST31
Repeati296 – 329WD 6Add BLAST34
Repeati341 – 373WD 7Add BLAST33

Sequence similaritiesi

Belongs to the WD repeat WDR5/wds family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0266. Eukaryota.
ENOG410XP3K. LUCA.
GeneTreeiENSGT00810000125363.
HOGENOMiHOG000091642.
InParanoidiQ17963.
KOiK14963.
OMAiCIKTLPA.
OrthoDBiEOG091G0CM2.
PhylomeDBiQ17963.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSENAASA AEQQPTQQID QLTVPNAPDG GSSAPAPSTS PNSISPSNPT
60 70 80 90 100
GTPAPGASAQ TPNPNAAGAS ASGSANYKLM CTLEGHTKSI SSAKFSPCGK
110 120 130 140 150
YLGTSSADKT VKIWNMDHMI CERTLTGHKL GVNDIAWSSD SRCVVSASDD
160 170 180 190 200
KTLKIFEIVT SRMTKTLKGH NNYVFCCNFN PQSSLVVSGS FDESVRIWDV
210 220 230 240 250
KTGMCIKTLP AHSDPVSAVS FNRDGSLIAS GSYDGLVRIW DTANGQCIKT
260 270 280 290 300
LVDDENPPVA FVKFSPNGKY ILASNLDSTL KLWDFSKGKT LKQYTGHENS
310 320 330 340 350
KYCIFANFSV TGGKWIISGS EDCKIYIWNL QTREIVQCLE GHTQPVLASD
360 370
CHPVQNIIAS GALEPDNKIH IWRSDV
Length:376
Mass (Da):40,393
Last modified:November 1, 1996 - v1
Checksum:i3204DC36FE58FC19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37139 Genomic DNA. Translation: CAA85487.1.
PIRiT19266.
RefSeqiNP_497749.1. NM_065348.7.
UniGeneiCel.22551.

Genome annotation databases

EnsemblMetazoaiC14B1.4; C14B1.4; WBGene00006474.
GeneIDi175474.
KEGGicel:CELE_C14B1.4.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37139 Genomic DNA. Translation: CAA85487.1.
PIRiT19266.
RefSeqiNP_497749.1. NM_065348.7.
UniGeneiCel.22551.

3D structure databases

ProteinModelPortaliQ17963.
SMRiQ17963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-55118N.
IntActiQ17963. 1 interactor.
STRINGi6239.C14B1.4.

Proteomic databases

EPDiQ17963.
PaxDbiQ17963.
PeptideAtlasiQ17963.
PRIDEiQ17963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC14B1.4; C14B1.4; WBGene00006474.
GeneIDi175474.
KEGGicel:CELE_C14B1.4.

Organism-specific databases

CTDi175474.
WormBaseiC14B1.4; CE00901; WBGene00006474; wdr-5.1.

Phylogenomic databases

eggNOGiKOG0266. Eukaryota.
ENOG410XP3K. LUCA.
GeneTreeiENSGT00810000125363.
HOGENOMiHOG000091642.
InParanoidiQ17963.
KOiK14963.
OMAiCIKTLPA.
OrthoDBiEOG091G0CM2.
PhylomeDBiQ17963.

Enzyme and pathway databases

SignaLinkiQ17963.

Miscellaneous databases

PROiQ17963.

Gene expression databases

BgeeiWBGene00006474.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWDR51_CAEEL
AccessioniPrimary (citable) accession number: Q17963
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.