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Reviewed, UniProtKB/Swiss-Prot Q17941 (AKT1_CAEEL)

Last modified November 25, 2008. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase akt-1
    EC=2.7.11.1
Alternative name(s):
    Protein kinase B akt-1
      Short name=PKB akt-1
Gene names
Name: akt-1
ORF Names: C12D8.10
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Acts downstream age-1 and pdk-1 in the daf-2/insulin receptor-like transduction pathway, which controls longevity and prevents developmental arrest at the dauer stage. Phosphorylates Forkhead-related daf-16 transcription factor, which inhibits its entry into the nucleus and antagonizes its function.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with pdk-1, sgk-1, akt-2 and daf-16. Part of a complex containing sgk-1, akt-1 and akt-2.

Tissue specificity

Expressed in neurons, muscle cells of the pharynx, rectal gland cells, and spermatheca.

Developmental stage

Expressed in late stage embryos and throughout life.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PH domain.

Contains 1 protein kinase domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

daf-16O186761EBI-1770718,EBI-1770827
skn-1P34707-11EBI-1770718,EBI-434984
skn-1P34707-31EBI-1770718,EBI-434993

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q17941-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q17941-2)

The sequence of this isoform differs from the canonical sequence as follows:
     262-287: EQHYLCFVMQFANGGELFTHVRKCGT → TNDRLCFVMEFAIGGDLYYHLNREVQMNKEG
     298-298: A → S
     309-317: RCDIVYRDM → ANSIVYRDL

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 541541Serine/threonine-protein kinase akt-1
PRO_0000085615

Regions

Domain15 – 118104PH
Domain193 – 450258Protein kinase
Domain451 – 52878AGC-kinase C-terminal
Nucleotide binding199 – 2079ATP By similarity

Sites

Active site3161Proton acceptor By similarity
Binding site2221ATP By similarity

Natural variations

Alternative sequence262 – 28726EQHYL…RKCGT → TNDRLCFVMEFAIGGDLYYH LNREVQMNKEG in isoform b.
VSP_017044
Alternative sequence2981A → S in isoform b.
VSP_017045
Alternative sequence309 – 3179RCDIVYRDM → ANSIVYRDL in isoform b.
VSP_017046

Experimental info

Mutagenesis1831A → T in mg144; suppresses the dauer arrest phenotype of the age-1(mg44) null mutant

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Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified August 1, 1998. Version 2.
Checksum: 17FEDD1109926950

FASTA54162,200
        10         20         30         40         50         60 
MSMTSLSTKS RRQEDVVIEG WLHKKGEHIR NWRPRYFMIF NDGALLGFRA KPKEGQPFPE 

        70         80         90        100        110        120 
PLNDFMIKDA ATMLFEKPRP NMFMVRCLQW TTVIERTFYA ESAEVRQRWI HAIESISKKY 

       130        140        150        160        170        180 
KGTNANPQEE LMETNQQPKI DEDSEFAGAA HAIMGQPSSG HGDNCSIDFR ASMISIADTS 

       190        200        210        220        230        240 
EAAKRDKITM EDFDFLKVLG KGTFGKVILC KEKRTQKLYA IKILKKDVII AREEVAHTLT 

       250        260        270        280        290        300 
ENRVLQRCKH PFLTELKYSF QEQHYLCFVM QFANGGELFT HVRKCGTFSE PRARFYGAEI 

       310        320        330        340        350        360 
VLALGYLHRC DIVYRDMKLE NLLLDKDGHI KIADFGLCKE EISFGDKTST FCGTPEYLAP 

       370        380        390        400        410        420 
EVLDDHDYGR CVDWWGVGVV MYEMMCGRLP FYSKDHNKLF ELIMAGDLRF PSKLSQEART 

       430        440        450        460        470        480 
LLTGLLVKDP TQRLGGGPED ALEICRADFF RTVDWEATYR KEIEPPYKPN VQSETDTSYF 

       490        500        510        520        530        540 
DNEFTSQPVQ LTPPSRSGAL ATVDEQEEMQ SNFTQFSFHN VMGSINRIHE ASEDNEDYDM 


G

« Hide

Isoform b [UniParc].

Checksum: 9719098DBED87AFB
Show »

54662,743

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References

« Hide 'large scale' references
[1]"Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor."
Paradis S., Ruvkun G.
Genes Dev. 12:2488-2498(1998) [PubMed: 9716402] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, MUTAGENESIS OF ALA-183, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans."
Paradis S., Ailion M., Toker A., Thomas J.H., Ruvkun G.
Genes Dev. 13:1438-1452(1999) [PubMed: 10364160] [Abstract]
Cited for: FUNCTION.
[4]"daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans."
Henderson S.T., Johnson T.E.
Curr. Biol. 11:1975-1980(2001) [PubMed: 11747825] [Abstract]
Cited for: FUNCTION.
[5]"Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling."
Lin K., Hsin H., Libina N., Kenyon C.
Nat. Genet. 28:139-145(2001) [PubMed: 11381260] [Abstract]
Cited for: FUNCTION.
[6]"C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life span."
Hertweck M., Goebel C., Baumeister R.
Dev. Cell 6:577-588(2004) [PubMed: 15068796] [Abstract]
Cited for: INTERACTION WITH PDK-1; SGK-1; AKT-2 AND DAF-16, FUNCTION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF072379 mRNA. Translation: AAC62466.1.
AF072380 mRNA. Translation: AAC62467.1.
Z73969 Genomic DNA. Translation: CAA98238.1.
Z73969 Genomic DNA. Translation: CAA98240.1.
PIRT43232.
T43233.
RefSeqNP_001023645.1.
UniGeneCel.5036

3D structure databases

HSSPHSSP built from PDB template 1MRY based on UniProtKB P31751.
ModBaseSearch...

Protein-protein interaction databases

IntActQ17941.

Genome annotation databases

EnsemblC12D8.10. Caenorhabditis elegans. [Contig view]
GeneID179424.
KEGGcel:C12D8.10.
NMPDRfig|6239.3.peg.19363.

Organism-specific databases

WormBaseWBGene00000102. akt-1.
WormPepC12D8.10a. CE15612. [WorfDB]
C12D8.10b. CE05274. [WorfDB]

Gene expression databases

ArrayExpressQ17941.

Family and domain databases

InterProIPR015744. Akt.
IPR001849. PH.
IPR011993. PH_type.
IPR000961. Pkinase_C.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_bd_CS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 1 hit.
PANTHERPTHR22985:SF69. Akt. 1 hit.
PfamPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio905330.

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Entry information

Entry nameAKT1_CAEEL
AccessionPrimary (citable) accession number: Q17941
Secondary accession number(s): Q17942
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: August 1, 1998
Last modified: November 25, 2008
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents