Serine/threonine-protein kinase akt-1 - Caenorhabditis elegans

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Reviewed, UniProtKB/Swiss-Prot Q17941 (AKT1_CAEEL)

Last modified January 19, 2010. Version 85. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase akt-1
    EC=2.7.11.1
Alternative name(s):
    Protein kinase B akt-1
      Short name=PKB akt-1

Gene names

Name:	akt-1
ORF Names:	C12D8.10

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Acts downstream of age-1 and pdk-1 in the daf-2/insulin receptor-like transduction pathway. Essential role in regulating developmental arrest at the dauer stage. Phosphorylates Forkhead-related daf-16 and the longevity-promoting skn-1 transcription factors, which inhibits their entry into the nucleus and antagonizes their functions. Role in immune function and pathogen resistance. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Enzyme regulation	Phosphorylated and activated by pdk-1. Ref.6
Subunit structure	Interacts with pdk-1, sgk-1, akt-2 and daf-16. Part of a complex containing sgk-1, akt-1 and akt-2. Ref.6
Tissue specificity	Expressed in neurons, muscle cells of the pharynx, rectal gland cells, and spermatheca. Ref.1
Developmental stage	Expressed in late stage embryos and throughout life. Ref.1
Disruption phenotype	Increased resistance to pathogens. Simultaneous knockdown of akt-1 and akt-2 result in dauer formation and a weak extension to life span. Ref.6 Ref.7
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain.

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Ontologies

Keywords
Biological process	Immune response
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dauer entry Ref.6 Inferred from mutant phenotype. Source: WormBase determination of adult lifespan Ref.6 Inferred from mutant phenotype. Source: WormBase immune response Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.6 Ref.8 Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
akt-2	Q9XTG7	1	EBI-1770718,EBI-320656
daf-16	O16850	3	EBI-1770718,EBI-324028
pdk-1	Q9Y1J3	1	EBI-1770718,EBI-323772
pptr-1	O18178	1	EBI-1770718,EBI-2298122
sgk-1	Q2PJ68	1	EBI-1770718,EBI-1770776
skn-1	P34707-1	1	EBI-1770718,EBI-434984
skn-1	P34707-3	1	EBI-1770718,EBI-434993

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q17941-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q17941-2) The sequence of this isoform differs from the canonical sequence as follows: 262-287: EQHYLCFVMQFANGGELFTHVRKCGT → TNDRLCFVMEFAIGGDLYYHLNREVQMNKEG 298-298: A → S 309-317: RCDIVYRDM → ANSIVYRDL

Isoform c (identifier: Q17941-3) The sequence of this isoform differs from the canonical sequence as follows: 255-541: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 541

541

Serine/threonine-protein kinase akt-1

PRO_0000085615

Regions

Domain

15 – 118

104

Domain

193 – 450

258

Protein kinase

Domain

451 – 528

AGC-kinase C-terminal

Nucleotide binding

199 – 207

ATP By similarity

Sites

Active site

316

Proton acceptor By similarity

Binding site

222

ATP By similarity

Natural variations

Alternative sequence

255 – 541

287

Missing in isoform c.

VSP_038163

Alternative sequence

262 – 287

EQHYL…RKCGT → TNDRLCFVMEFAIGGDLYYH LNREVQMNKEG in isoform b.

VSP_017044

Alternative sequence

298

A → S in isoform b.

VSP_017045

Alternative sequence

309 – 317

RCDIVYRDM → ANSIVYRDL in isoform b.

VSP_017046

Experimental info

Mutagenesis

183

A → T in mg144; suppresses the dauer arrest phenotype of the age-1(mg44) null mutant. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform a [UniParc]. Last modified August 1, 1998. Version 2. Checksum: 17FEDD1109926950 Show »	FASTA	541	62,200
10 20 30 40 50 60 MSMTSLSTKS RRQEDVVIEG WLHKKGEHIR NWRPRYFMIF NDGALLGFRA KPKEGQPFPE 70 80 90 100 110 120 PLNDFMIKDA ATMLFEKPRP NMFMVRCLQW TTVIERTFYA ESAEVRQRWI HAIESISKKY 130 140 150 160 170 180 KGTNANPQEE LMETNQQPKI DEDSEFAGAA HAIMGQPSSG HGDNCSIDFR ASMISIADTS 190 200 210 220 230 240 EAAKRDKITM EDFDFLKVLG KGTFGKVILC KEKRTQKLYA IKILKKDVII AREEVAHTLT 250 260 270 280 290 300 ENRVLQRCKH PFLTELKYSF QEQHYLCFVM QFANGGELFT HVRKCGTFSE PRARFYGAEI 310 320 330 340 350 360 VLALGYLHRC DIVYRDMKLE NLLLDKDGHI KIADFGLCKE EISFGDKTST FCGTPEYLAP 370 380 390 400 410 420 EVLDDHDYGR CVDWWGVGVV MYEMMCGRLP FYSKDHNKLF ELIMAGDLRF PSKLSQEART 430 440 450 460 470 480 LLTGLLVKDP TQRLGGGPED ALEICRADFF RTVDWEATYR KEIEPPYKPN VQSETDTSYF 490 500 510 520 530 540 DNEFTSQPVQ LTPPSRSGAL ATVDEQEEMQ SNFTQFSFHN VMGSINRIHE ASEDNEDYDM G « Hide
Isoform b. Checksum: 9719098DBED87AFB Show »	FASTA	546	62,743
10 20 30 40 50 60 MSMTSLSTKS RRQEDVVIEG WLHKKGEHIR NWRPRYFMIF NDGALLGFRA KPKEGQPFPE 70 80 90 100 110 120 PLNDFMIKDA ATMLFEKPRP NMFMVRCLQW TTVIERTFYA ESAEVRQRWI HAIESISKKY 130 140 150 160 170 180 KGTNANPQEE LMETNQQPKI DEDSEFAGAA HAIMGQPSSG HGDNCSIDFR ASMISIADTS 190 200 210 220 230 240 EAAKRDKITM EDFDFLKVLG KGTFGKVILC KEKRTQKLYA IKILKKDVII AREEVAHTLT 250 260 270 280 290 300 ENRVLQRCKH PFLTELKYSF QTNDRLCFVM EFAIGGDLYY HLNREVQMNK EGFSEPRARF 310 320 330 340 350 360 YGSEIVLALG YLHANSIVYR DLKLENLLLD KDGHIKIADF GLCKEEISFG DKTSTFCGTP 370 380 390 400 410 420 EYLAPEVLDD HDYGRCVDWW GVGVVMYEMM CGRLPFYSKD HNKLFELIMA GDLRFPSKLS 430 440 450 460 470 480 QEARTLLTGL LVKDPTQRLG GGPEDALEIC RADFFRTVDW EATYRKEIEP PYKPNVQSET 490 500 510 520 530 540 DTSYFDNEFT SQPVQLTPPS RSGALATVDE QEEMQSNFTQ FSFHNVMGSI NRIHEASEDN EDYDMG « Hide
Isoform c. Checksum: BC1E384AB75A7C77 Show »	FASTA	254	29,259
10 20 30 40 50 60 MSMTSLSTKS RRQEDVVIEG WLHKKGEHIR NWRPRYFMIF NDGALLGFRA KPKEGQPFPE 70 80 90 100 110 120 PLNDFMIKDA ATMLFEKPRP NMFMVRCLQW TTVIERTFYA ESAEVRQRWI HAIESISKKY 130 140 150 160 170 180 KGTNANPQEE LMETNQQPKI DEDSEFAGAA HAIMGQPSSG HGDNCSIDFR ASMISIADTS 190 200 210 220 230 240 EAAKRDKITM EDFDFLKVLG KGTFGKVILC KEKRTQKLYA IKILKKDVII AREEVAHTLT 250 ENRVLQRCKH PFLT « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor." Paradis S., Ruvkun G. Genes Dev. 12:2488-2498(1998) [PubMed: 9716402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, MUTAGENESIS OF ALA-183, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans." Paradis S., Ailion M., Toker A., Thomas J.H., Ruvkun G. Genes Dev. 13:1438-1452(1999) [PubMed: 10364160] [Abstract] Cited for: FUNCTION.
[4]	"daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans." Henderson S.T., Johnson T.E. Curr. Biol. 11:1975-1980(2001) [PubMed: 11747825] [Abstract] Cited for: FUNCTION.
[5]	"Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling." Lin K., Hsin H., Libina N., Kenyon C. Nat. Genet. 28:139-145(2001) [PubMed: 11381260] [Abstract] Cited for: FUNCTION.
[6]	"C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life span." Hertweck M., Goebel C., Baumeister R. Dev. Cell 6:577-588(2004) [PubMed: 15068796] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH PDK-1; SGK-1; AKT-2 AND DAF-16, DISRUPTION PHENOTYPE.
[7]	"The DAF-2 insulin-like signaling pathway independently regulates aging and immunity in C. elegans." Evans E.A., Chen W.C., Tan M.-W. Aging Cell 7:879-893(2008) [PubMed: 18782349] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans." Tullet J.M., Hertweck M., An J.H., Baker J., Hwang J.Y., Liu S., Oliveira R.P., Baumeister R., Blackwell T.K. Cell 132:1025-1038(2008) [PubMed: 18358814] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AF072379 mRNA. Translation: AAC62466.1. AF072380 mRNA. Translation: AAC62467.1. Z73969 Genomic DNA. Translation: CAA98238.1. Z73969 Genomic DNA. Translation: CAA98240.1. Z73969 Genomic DNA. Translation: CAD44085.1.
PIR	T43232. T43233.
RefSeq	NP_001023645.1. NP_001023646.1. NP_001023647.1.
UniGene	Cel.5036
3D structure databases
SMR	Q17941. Positions 10-122, 187-491, 190-520.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q17941. 7 interactions.
STRING	Q17941.
Proteomic databases
PRIDE	Q17941.
Genome annotation databases
Ensembl	C12D8.10a.1; C12D8.10a.1; C12D8.10; Caenorhabditis elegans. [Genome view] C12D8.10a.2; C12D8.10a.2; C12D8.10; Caenorhabditis elegans. [Genome view]
GeneID	179424.
KEGG	cel:C12D8.10.
NMPDR	fig\|6239.3.peg.19363.
UCSC	C12D8.10b.1. c. elegans.
Organism-specific databases
CTD	179424.
WormBase	WBGene00000102. akt-1.
WormPep	C12D8.10a. CE15612. [WorfDB] C12D8.10b. CE05274. [WorfDB] C12D8.10c. CE31304. [WorfDB]
Phylogenomic databases
eggNOG	meNOG06525.
InParanoid	Q17941.
OMA	LCFVMEF.
PhylomeDB	Q17941.
Enzyme and pathway databases
BRENDA	2.7.11.1. 672.
Gene expression databases
ArrayExpress	Q17941.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR017892. Pkinase_C. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. IPR015744. Serine/threonine_Kinase_Rac. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR22985:SF69. Akt. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	905330.

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Entry information

Entry name

AKT1_CAEEL

Accession

Primary (citable) accession number: Q17941
Secondary accession number(s): Q17942, Q8MQE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	August 1, 1998
Last modified:	January 19, 2010
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents